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Biology of Reproduction, Vol 45, 381-386, Copyright © 1991 by Society for the Study of Reproduction

ARTICLES

Arylsulfatases are present in seminal plasma of several domestic mammals

BM Gadella, B Colenbrander and M Lopes-Cardozo
Department of Herd Health and Reproduction, Veterinary School Utrecht University, The Netherlands.

Mammalian spermatozoa and seminal plasma both contain high levels of arylsulfatases (AS), enzymes that remove sulfate from sulfated glycoconjugates. In ejaculated semen of boars, 85% of AS was found in seminal plasma whereas only 13% was found in spermatozoa. A comparable distribution of AS between spermatozoa and seminal plasma was observed in other domestic mammals. The presence of AS in seminal plasma was not due to leakage from spermatozoa because sperm cells had intact acrosomes and plasma membranes after their separation from seminal plasma, and because 84% of the acrosomal marker enzyme hyaluronidase was retained in washed spermatozoa. Spermatozoa in boar semen diluted with Beltsville Thawing Solution (BTS) deteriorated faster during storage at 17 degrees C than spermatozoa stored in BTS without seminal plasma. This suggests that seminal plasma has a deleterious effect on mammalian spermatozoa. We propose that (1) sulfated glycoconjugates stabilize sperm plasma membranes; (2) AS present in seminal plasma contribute to the deterioration of spermatozoa by desulfating these glycoconjugates; and (3) AS present in seminal plasma could well play a role in sperm capacitation.


This article has been cited by other articles:


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B. Gadella, M Lopes-Cardozo, L. van Golde, B Colenbrander, and T. Gadella
Glycolipid migration from the apical to the equatorial subdomains of the sperm head plasma membrane precedes the acrosome reaction. Evidence for a primary capacitation event in boar spermatozoa
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[Abstract] [PDF]

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B. Gadella, T. Gadella, B Colenbrander, L. van Golde, and M Lopes-Cardozo
Visualization and quantification of glycolipid polarity dynamics in the plasma membrane of the mammalian spermatozoon
J. Cell Sci., January 8, 1994; 107(8): 2151 - 2163.
[Abstract] [PDF]


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Copyright © 1991 by the Society for the Study of Reproduction.