Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 45, 755-763, Copyright © 1991 by Society for the Study of Reproduction

ARTICLES

Effect of sampling interval on serum concentrations of luteinizing hormone, follicle-stimulating hormone, and prolactin in prepubertal, ovariectomized, and cycling gilts

MA Diekman, ML Green, TE Fears, JR Malayer and T Kuczek
Department of Animal Sciences, Purdue University, West Lafayette, Indiana 47907.

Three experiments were conducted to determine the effect of sampling interval on serum concentrations of LH, FSH, and prolactin (PRL) in prepubertal, ovariectomized, and cycling gilts. In all experiments, blood samples were drawn at 2-min intervals for 4 h from indwelling jugular catheters. Mean serum hormone concentrations, mean number of peaks, and mean and maximum peak heights of LH, FSH, and PRL were calculated using values reflecting 2-, 6-, 10-, 20-, 30-, and 60-min sampling intervals. For LH, FSH, and PRL, mean serum concentrations can be obtained through blood samples drawn at hourly intervals. Since LH peaks are very distinct in pigs, the number of secretory peaks and mean peak height can be obtained via samples drawn at 20-min intervals. Since FSH and PRL peaks are less well defined, a more frequent sampling interval (10 min) is needed to determine number of peaks and mean peak height. To obtain the maximum peak height or the number of minutes for LH, FSH, or PRL to rise from its nadir to zenith, blood samples need to be drawn at 2-min intervals. Regardless of reproductive state, these data indicate that the sampling interval needed to characterize serum concentrations of LH, FSH, and PRL in the gilt is dependent upon the parameter in question.


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J. A Clapper
Effects of two different anaesthetics on serum concentrations of cortisol and luteinizing hormone in barrows and gilts
Lab Anim, January 1, 2008; 42(1): 83 - 91.
[Abstract] [Full Text] [PDF]


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Copyright © 1991 by the Society for the Study of Reproduction.