Biology of Reproduction, Vol 46, 595-606, Copyright © 1992 by Society for the Study of Reproduction

ARTICLES

Cell-type-specific localization of transforming growth factor-beta 2 and transforming growth factor-beta 1 in the hamster ovary: differential regulation by follicle-stimulating hormone and luteinizing hormone

SK Roy, C Ogren, C Roy and B Lu
Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha 68198-4515.

Cell-type-specific localization and gonadotropin regulation of transforming growth factor-beta 1 (TGF-beta 1) and transforming growth factor-beta 2 (TGF-beta 2) in the hamster ovary were evaluated immunohistochemically under three conditions: (1) during the estrous cycle (Day 1 = estrus; Day 4 = proestrus); (2) after the blockade of periovulatory gonadotropin surges by phenobarbital, and (3) after FSH and/or LH treatment of long-term hypophysectomized hamsters. Ovarian TGF-beta 1 activity was primarily localized in theca and interstitial cells. The activity increased moderately but significantly after the preovulatory LH surge and reached a peak at 0900 h, Day 2 h; oocytes showed considerable activity. TGF-beta 1 immunoreactivity subsequently fell to low levels in theca-interstitial cells through 0900 h, Day 4. Significant TGF-beta 2 immunoreactivity appeared after the surge, mainly in the granulosa cells of both preantral and antral follicles; a few interstitial cells surrounding preantral follicles showed discrete staining. TGF-beta 2 immunoreactivity in granulosa cells and in interstitial cells next to preantral follicles reached a peak at 0900 h, Day 1, and persisted up to 0900 h, Day 2; oocytes showed no staining. Phenobarbital treatment blocked the appearance of TGF-beta 1 and TGF-beta 2 immunoreactivities at 1600 h, Day 4; however, a rebound in immunoreactivities was observed with the onset of the surge after a 1-day delay. Replacement of LH to long-term hypophysectomized hamsters resulted in a marked increase in TGF-beta 1 immunoreactivity in the interstitial cells, but FSH, although it induced follicular development, did not influence ovarian TGF-beta 1 activity. Treatment with FSH, however, induced a massive increase in TGF-beta 2 immunoreactivity in the granulosa cells of newly developed antral and preantral follicles but not in the interstitial cells; LH, on the other hand, had no significant effect on TGF-beta 2 activity. Treatment with FSH and LH combined resulted in a dramatic increase in TGF-beta 2 immunoreactivity in granulosa and interstitial cells and in TGF-beta 1 in theca and interstitial cells comparable to their peak activity in intact animals. Western analyses substantiated the presence of TGF-beta 1 and TGF-beta 2 in the hamster ovary and the specificity of immunolocalization. These studies, therefore, provide critical evidence that TGF-beta 1 and TGF-beta 2 in the hamster ovary are expressed in specific cell types and that their expression is differentially regulated by LH and FSH, respectively.

This article has been cited by other articles:

				T. Adriaenssens, C. Mazoyer, I. Segers, S. Wathlet, and J. Smitz Differences in Collagen Expression in Cumulus Cells after Exposure to Highly Purified Menotropin or Recombinant Follicle-Stimulating Hormone in a Mouse Follicle Culture Model Biol Reprod, May 1, 2009; 80(5): 1015 - 1025. [Abstract] [Full Text] [PDF]

				D. Rosairo, I. Kuyznierewicz, J. Findlay, and A. Drummond Transforming growth factor-{beta}: its role in ovarian follicle development Reproduction, December 1, 2008; 136(6): 799 - 809. [Abstract] [Full Text] [PDF]

				C. Wang and S. K. Roy Development of Primordial Follicles in the Hamster: Role of Estradiol-17{beta} Endocrinology, April 1, 2007; 148(4): 1707 - 1716. [Abstract] [Full Text] [PDF]

				P. Yang and S. K. Roy Transforming Growth Factor B1 Stimulated DNA Synthesis in the Granulosa Cells of Preantral Follicles: Negative Interaction with Epidermal Growth Factor Biol Reprod, July 1, 2006; 75(1): 140 - 148. [Abstract] [Full Text] [PDF]

				W. V. Ingman, R. L. Robker, K. Woittiez, and S. A. Robertson Null Mutation in Transforming Growth Factor {beta}1 Disrupts Ovarian Function and Causes Oocyte Incompetence and Early Embryo Arrest Endocrinology, February 1, 2006; 147(2): 835 - 845. [Abstract] [Full Text] [PDF]

				X. Gueripel, M. Benahmed, and A. Gougeon Sequential Gonadotropin Treatment of Immature Mice Leads to Amplification of Transforming Growth Factor {beta} Action, Via Upregulation of Receptor-Type 1, Smad 2 and 4, and Downregulation of Smad 6 Biol Reprod, March 1, 2004; 70(3): 640 - 648. [Abstract] [Full Text] [PDF]

				S. K. Roy, J. Wang, and P. Yang Dexamethasone Inhibits Transforming Growth Factor-{beta} Receptor (T{beta}R) Messenger RNA Expression in Hamster Preantral Follicles: Possible Association with NF-YA Biol Reprod, June 1, 2003; 68(6): 2180 - 2188. [Abstract] [Full Text] [PDF]

				G. Kohli, S. Hu, E. Clelland, T. Di Muccio, J. Rothenstein, and C. Peng Cloning of Transforming Growth Factor-{beta}1 (TGF-{beta}1) and Its Type II Receptor from Zebrafish Ovary and Role of TGF-{beta}1 in Oocyte Maturation Endocrinology, May 1, 2003; 144(5): 1931 - 1941. [Abstract] [Full Text] [PDF]

				P. Yang and S. K. Roy Epidermal Growth Factor Modulates Transforming Growth Factor Receptor Messenger RNA and Protein Levels in Hamster Preantral Follicles In Vitro Biol Reprod, September 1, 2001; 65(3): 847 - 854. [Abstract] [Full Text] [PDF]

				S. K. Roy Regulation of Transforming Growth Factor-{beta}-Receptor Type I and Type II Messenger Ribonucleic Acid Expression in the Hamster Ovary by Gonadotropinsand Steroid Hormones Biol Reprod, June 1, 2000; 62(6): 1858 - 1865. [Abstract] [Full Text]

				N. Yu and S. K. Roy Development of Primordial and Prenatal Follicles from Undifferentiated Somatic Cells and Oocytes in the Hamster Prenatal Ovary In Vitro: Effect of Insulin Biol Reprod, December 1, 1999; 61(6): 1558 - 1567. [Abstract] [Full Text]

				S. K. Roy and D. M. Terada Activities of Glucose Metabolic Enzymes in Human Preantral Follicles: In Vitro Modulation by Follicle-Stimulating Hormone, Luteinizing Hormone, Epidermal Growth Factor, Insulin-Like Growth Factor I, and Transforming Growth Factor ß1 Biol Reprod, March 1, 1999; 60(3): 763 - 768. [Abstract] [Full Text]

				S. K. Roy, S. G. Kurz, A. M. Carlson, C. J. DeJonge, J. W. Ramey, and V. M. Maclin Transforming Growth Factor ß Receptor Expression in Hyperstimulated Human Granulosa Cells and Cleavage Potential of the Zygotes Biol Reprod, December 1, 1998; 59(6): 1311 - 1316. [Abstract] [Full Text]

				B. Bieser,, M. Stojkovic,, E. Wolf,, H. Meyer,, and R. Einspanier Growth Factors and Components for Extracellular Proteolysis Are Differentially Expressed during In Vitro Maturation of Bovine Cumulus-Oocyte Complexes Biol Reprod, October 1, 1998; 59(4): 801 - 806. [Abstract] [Full Text]