Biol Reprod 2009 SSR Annual Meeting Abstracts
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Biology of Reproduction, Vol 47, 588-597, Copyright © 1992 by Society for the Study of Reproduction

ARTICLES

Binding of epididymal proteins to rat spermatozoa in vivo

JT Vreeburg, MK Holland and MC Orgebin-Crist
Department of Endocrinology and Reproduction, Faculty of Medicine, Erasmus University, Rotterdam, The Netherlands.

The secretion of epididymal proteins and their binding to spermatozoa in rats were examined after retrograde perfusion of the superior and inferior epididymal arteries with [35S]methionine. PAGE revealed that the pattern of radioactive proteins in the luminal fluid was markedly different from the well-characterized pattern of secretory proteins obtained by in vitro incubation of epididymal minces with labeled methionine. Of the proteins secreted into the lumen, about 1% were associated with Percoll-purified spermatozoa. More proteins were associated with the spermatozoa in the corpus epididymidis than in the caput. Sequential extraction of spermatozoa with an isotonic buffer, a high-salt buffer, Triton X-100, and SDS revealed that almost half of the radiolabeled proteins could be extracted with the isotonic buffer. The firmly bound radioactive proteins remaining, which were extracted with Triton X-100 or SDS, consisted of one major band of 25 kDa and two minor bands of 30 kDa and 32 kDa. Analysis of the sperm-associated proteins at various times after the isotope was administered indicated that tight binding of proteins to spermatozoa occurs within 3 h after isotope injection.


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Copyright © 1992 by the Society for the Study of Reproduction.