Biology of Reproduction, Vol 50, 215-221, Copyright © 1994 by Society for the Study of Reproduction

ARTICLES

Beta-adrenergic receptors in corpora lutea from different stages of the estrous cycle in conscious and slaughtered cattle

M Pesta, A Muszynska, J Kucharski, J Superata and J Kotwica
Division of Animal Reproduction Endocrinology and Pathophysiology, Polish Academy of Sciences, University of Agriculture and Technology, Olsztyn-Kortowo.

The overall objective of this study was to determine the pattern of beta-adrenergic receptors in bovine luteal membranes obtained from slaughtered and from conscious cattle on different days of the estrous cycle. Two approaches were used with respect to the CL collection. In the first approach, CL (n = 20) were obtained from animals slaughtered in a local abattoir. The day of the estrous cycle was estimated based on the morphology of luteal tissue and follicles present. Using 3H- dihydroalprenolol (3H-DHA) as a ligand, we were unable to find specific binding in any of the tissues examined. Therefore, for the second approach we decided to collect CL from mature heifers or cows under local anesthesia on Days 4 (n = 15), 8 (n = 7), 12 (n = 6), and 16 (n = 3) of the estrous cycle by means of colpotomy or by lumbar incision. This procedure was chosen to reduce the influence of stress factors before CL collection. In these samples, specific beta-adrenergic binding sites were found and they varied during the estrous cycle. Maximal binding (Bmax; fmol/mg of protein) was highest on Day 4 (178.3 +/- 15.2); it then decreased significantly (73.2 +/- 14.7, 40.1 +/- 5.5, 10.8 +/- 0.8) on Days 8, 12, and 16, respectively. The dissociation constant (Kd; nM), which followed the same pattern, was 52.0 +/- 14.4, 16.3 +/- 3.9, 14.9 +/- 3.2, and 7.6 +/- 4.0) on the same days, respectively.(ABSTRACT TRUNCATED AT 250 WORDS)

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