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Biology of Reproduction, Vol 50, 349-356, Copyright © 1994 by Society for the Study of Reproduction

ARTICLES

Changes in levels of messenger ribonucleic acid for cytochrome P450 side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase during prostaglandin F2 alpha-induced luteolysis in cattle

XC Tian, AK Berndtson and JE Fortune
Department and Section of Physiology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853.

Regression of the CL causes a dramatic decrease in plasma progesterone levels. To test the hypothesis that the decrease in progesterone involves the down-regulation of mRNA encoding the steroidogenic enzymes, cytochrome P450 side-chain cleavage (P450scc) and/or 3 beta- hydroxysteroid dehydrogenase (3 beta-HSD), levels of plasma progesterone and luteal mRNA for P450scc and 3 beta-HSD were measured and correlated during induced luteolysis. Holstein heifers (n = 25) were injected with 25 mg prostaglandin F2 alpha (PGF2 alpha) on Day 6 or 7 of the estrous cycle (Day 0 = estrus) to induce luteal regression. To determine acute changes in plasma progesterone during luteolysis, jugular blood samples were obtained from 5 heifers hourly for 12 h, beginning immediately before injection of PGF2 alpha, and assayed for progesterone by RIA. A significant decrease in plasma progesterone levels was observed as early as 1 h after the PGF2 alpha injection (3.62 vs. 2.72 ng/ml, p < 0.05). Progesterone levels continued to decline with time through 12 h after administration of PGF2 alpha. The other 20 animals were ovariectomized at 0 (n = 6), 2 (n = 4), 12 (n = 4), or 24 (n = 6) h after PGF2 alpha. Levels of P450scc and 3 beta-HSD mRNA were determined in extracts of total luteal RNA by ribonuclease (RNase) protection assay. By 2 h after PGF2 alpha, 3 beta-HSD mRNA levels had decreased by about 40% as compared with levels at time 0 (p < 0.05), and a further significant decrease occurred between 2 and 12 h.(ABSTRACT TRUNCATED AT 250 WORDS)


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