| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology of Reproduction, Vol 50, 390-400, Copyright © 1994 by Society for the Study of Reproduction
ARTICLES |
DK Gardner, M Lane, A Spitzer and PA Batt
Centre for Early Human Development, Monash University, Clayton, Victoria, Australia.
The aim of this study was to develop a serum-free culture system that could support high levels of cleavage and blastocyst formation from sheep zygotes developed in vitro. To this end, we investigated the effects on sheep zygote development of amino acids, ammonium, vitamins, and culture of embryos in groups in Synthetic Oviduct Fluid (SOF) medium supplemented with BSA (32 mg/ml). The inclusion of amino acids in the culture medium had no effect on the percentage of embryos arrested at the 8-16-cell stage when embryos were cultured singly in the same drop of medium for 6 days (43% in SOF; 41% in SOF+amino acids). However, in medium containing all Eagle's amino acids, replacing the culture medium every 48 h to alleviate ammonium toxicity significantly decreased the number of arrested embryos (6%; p < 0.05) and significantly increased blastocyst cell number (52 cells in SOF; 105 cells in SOF+amino acids; p < 0.01) and the number of embryos developing to the blastocyst stage (29% in SOF; 67% in SOF+amino acids; p < 0.05). When the medium was renewed every 48 h, nonessential amino acids and glutamine also significantly decreased the number of arrested embryos (p < 0.05). Culturing embryos singly or in groups in SOF medium with all Eagle's amino acids that was renewed every 48 h resulted in significant increases in blastocyst hatching and mean cell number (47%, 31%, and 79%; 105, 136, and 173 cells for embryos cultured singly, in groups of 2, and in groups of 4, respectively). After culture in groups of 4, blastocyst cell numbers were equivalent to in vivo-developed controls (160 cells) and significantly greater than those developed in serum (103 cells; p < 0.01). Analysis of blastocyst metabolism, expressed on a per-cell basis, revealed that amino acids did not affect either glucose uptake or lactate production, whereas the addition of amino acids and vitamins resulted in a significant increase in both parameters (p < 0.01). A similar response was observed in serum-derived blastocysts. Ammonium production by sheep blastocysts after culture in the presence of amino acids was significantly greater than that produced by mouse blastocysts, indirect evidence that ruminant embryos utilize amino acids to a greater extent than do rodent embryos.(ABSTRACT TRUNCATED AT 400 WORDS)
This article has been cited by other articles:
|
|
 |
|
  Y. Li, V. Chandrakanthan, M. L Day, and C. O'Neill Direct Evidence for the Action of Phosphatidylinositol (3,4,5)-Trisphosphate-Mediated Signal Transduction in the 2-Cell Mouse Embryo Biol Reprod, November 1, 2007; 77(5): 813 - 821. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Herrick, J. B. Bond, G. M. Magarey, H. L. Bateman, R. L. Krisher, S. A. Dunford, and W. F. Swanson Toward a Feline-Optimized Culture Medium: Impact of Ions, Carbohydrates, Essential Amino Acids, Vitamins, and Serum on Development and Metabolism of In Vitro Fertilization-Derived Feline Embryos Relative to Embryos Grown In Vivo Biol Reprod, May 1, 2007; 76(5): 858 - 870. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. E. Westhusin, T. Shin, J. W. Templeton, R. C. Burghardt, and L. G. Adams Rescuing valuable genomes by animal cloning: A case for natural disease resistance in cattle J Anim Sci, January 1, 2007; 85(1): 138 - 142. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-B. Han, G.-C. Lan, Y.-G. Wu, D. Han, W.-G. Feng, J.-Z. Wang, and J.-H. Tan Interactive effects of granulosa cell apoptosis, follicle size, cumulus-oocyte complex morphology, and cumulus expansion on the developmental competence of goat oocytes: a study using the well-in-drop culture system. Reproduction, November 1, 2006; 132(5): 749 - 758. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Lazzari, S. Colleoni, S. G. Giannelli, D. Brunetti, E. Colombo, I. Lagutina, C. Galli, and V. Broccoli Direct Derivation of Neural Rosettes from Cloned Bovine Blastocysts: A Model of Early Neurulation Events and Neural Crest Specification In Vitro Stem Cells, November 1, 2006; 24(11): 2514 - 2521. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. R Arnold, V. Bordignon, R. Lefebvre, B. D Murphy, and L. C Smith Somatic cell nuclear transfer alters peri-implantation trophoblast differentiation in bovine embryos. Reproduction, August 1, 2006; 132(2): 279 - 290. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Suteevun, R. Parnpai, S. L. Smith, C-C. Chang, S. Muenthaisong, and X. C. Tian Epigenetic characteristics of cloned and in vitro-fertilized swamp buffalo (Bubalus bubalis) embryos J Anim Sci, August 1, 2006; 84(8): 2065 - 2071. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Lloyd, J.-H. Lee, R. Alberio, E. J. Bowles, J. Ramalho-Santos, K. H. S. Campbell, and J. C. St. John Aberrant Nucleo-cytoplasmic Cross-Talk Results in Donor Cell mtDNA Persistence in Cloned Embryos Genetics, April 1, 2006; 172(4): 2515 - 2527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Lagutina, G. Lazzari, R. Duchi, S. Colleoni, N. Ponderato, P. Turini, G. Crotti, and C. Galli Somatic cell nuclear transfer in horses: effect of oocyte morphology, embryo reconstruction method and donor cell type Reproduction, October 1, 2005; 130(4): 559 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Miles, C. E. Farin, K. F. Rodriguez, J. E. Alexander, and P. W. Farin Effects of Embryo Culture on Angiogenesis and Morphometry of Bovine Placentas During Early Gestation Biol Reprod, October 1, 2005; 73(4): 663 - 671. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Ali and M.-A. Sirard Protein kinases influence bovine oocyte competence during short-term treatment with recombinant human follicle stimulating hormone Reproduction, September 1, 2005; 130(3): 303 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. T. Ruddock, K. J. Wilson, M. A. Cooney, N. A. Korfiatis, R. T. Tecirlioglu, and A. J. French Analysis of Imprinted Messenger RNA Expression During Bovine Preimplantation Development Biol Reprod, April 1, 2004; 70(4): 1131 - 1135. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Lagutina, G. Lazzari, R. Duchi, and C. Galli Developmental Potential of Bovine Androgenetic and Parthenogenetic Embryos: A Comparative Study Biol Reprod, February 1, 2004; 70(2): 400 - 405. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-S. Cui, Y.-J. Jeong, H.-Y. Lee, S.-H. Cheon, and N.-H. Kim Fetal bovine serum influences apoptosis and apoptosis-related gene expression in porcine parthenotes developing in vitro Reproduction, January 1, 2004; 127(1): 125 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Lane and D. K. Gardner Ammonium Induces Aberrant Blastocyst Differentiation, Metabolism, pH Regulation, Gene Expression and Subsequently Alters Fetal Development in the Mouse Biol Reprod, October 1, 2003; 69(4): 1109 - 1117. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Rizos, A. Gutierrez-Adan, S. Perez-Garnelo, J. de la Fuente, M.P. Boland, and P. Lonergan Bovine Embryo Culture in the Presence or Absence of Serum: Implications for Blastocyst Development, Cryotolerance, and Messenger RNA Expression Biol Reprod, January 1, 2003; 68(1): 236 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Van Thuan, H. Harayama, and M. Miyake Characteristics of Preimplantational Development of Porcine Parthenogenetic Diploids Relative to the Existence of Amino Acids In Vitro Biol Reprod, December 1, 2002; 67(6): 1688 - 1698. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N.S. Macklon, M.H.E.C. Pieters, M.A. Hassan, P.H.M. Jeucken, M.J.C. Eijkemans, and B.C.J.M. Fauser A prospective randomized comparison of sequential versus monoculture systems for in-vitro human blastocyst development Hum. Reprod., October 1, 2002; 17(10): 2700 - 2705. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Steinborn, P. Schinogl, D. N. Wells, A. Bergthaler, M. Muller, and G. Brem Coexistence of Bos taurus and B. indicus Mitochondrial DNAs in Nuclear Transfer-Derived Somatic Cattle Clones Genetics, October 1, 2002; 162(2): 823 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Utsunomiya, T. Naitou, and M. Nagaki A prospective trial of blastocyst culture and transfer Hum. Reprod., July 1, 2002; 17(7): 1846 - 1851. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-S. Xu, T.-M. Cheung, S. Ting-Hon Chan, P.-C. Ho, and W. Shu-Biu Yeung Temporal Effect of Human Oviductal Cell and Its Derived Embryotrophic Factors on Mouse Embryo Development Biol Reprod, November 1, 2001; 65(5): 1481 - 1488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Devreker, K. Hardy, M. Van den Bergh, A.S. Vannin, S. Emiliani, and Y. Englert Amino acids promote human blastocyst development in vitro Hum. Reprod., April 1, 2001; 16(4): 749 - 756. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.L. Keefer, H. Baldassarre, R. Keyston, B. Wang, B. Bhatia, A.S. Bilodeau, J.F. Zhou, M. Leduc, B.R. Downey, A. Lazaris, et al. Generation of Dwarf Goat (Capra hircus) Clones Following Nuclear Transfer with Transfected and Nontransfected Fetal Fibroblasts and In Vitro-Matured Oocytes Biol Reprod, March 1, 2001; 64(3): 849 - 856. [Abstract] [Full Text]
|
|
|
|
|
|
C. Anderiesz, A.-P. Ferraretti, C. Magli, A. Fiorentino, D. Fortini, L. Gianaroli, G.M. Jones, and A.O. Trounson Effect of recombinant human gonadotrophins on human, bovine and murine oocyte meiosis, fertilization and embryonic development in vitro Hum. Reprod., May 1, 2000; 15(5): 1140 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Hill, Q. A. Winger, C. R. Long, C. R. Looney, J. A. Thompson, and M. E. Westhusin Development Rates of Male Bovine Nuclear Transfer Embryos Derived from Adult and Fetal Cells Biol Reprod, May 1, 2000; 62(5): 1135 - 1140. [Abstract] [Full Text]
|
|
|
|
|
|
A. E. Crosier, P. W. Farin, M. J. Dykstra, J. E. Alexander, and C. E. Farin Ultrastructural Morphometry of Bovine Compact Morulae Produced In Vivo or In Vitro Biol Reprod, May 1, 2000; 62(5): 1459 - 1465. [Abstract] [Full Text]
|
|
|
|
|
|
R. Daniels, V. Hall, and A.O. Trounson Analysis of Gene Transcription in Bovine Nuclear Transfer Embryos Reconstructed with Granulosa Cell Nuclei Biol Reprod, April 1, 2000; 63(4): 1034 - 1040. [Abstract] [Full Text]
|
|
|
|
|
|
A.P. Gandhi, M. Lane, D.K. Gardner, and R.L. Krisher A single medium supports development of bovine embryos throughout maturation, fertilization and culture Hum. Reprod., February 1, 2000; 15(2): 395 - 401. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Watson, P. De Sousa, A. Caveney, L. C. Barcroft, D. Natale, J. Urquhart, and M. E. Westhusin Impact of Bovine Oocyte Maturation Media on Oocyte Transcript Levels, Blastocyst Development, Cell Number, and Apoptosis Biol Reprod, February 1, 2000; 62(2): 355 - 364. [Abstract] [Full Text]
|
|
|
|
|
|
P.M. Rijnders and C.A.M. Jansen Influence of group culture and culture volume on the formation of human blastocysts: a prospective randomized study Hum. Reprod., September 1, 1999; 14(9): 2333 - 2337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T.E. Steeves and D.K. Gardner Temporal and Differential Effects of Amino Acids on Bovine Embryo Development in Culture Biol Reprod, September 1, 1999; 61(3): 731 - 740. [Abstract] [Full Text]
|
|
|
|
|
|
M. A. Larson and H. M. Kubisch The effects of group size on development and interferon-{tau} secretion by in-vitro fertilized and cultured bovine blastocysts Hum. Reprod., August 1, 1999; 14(8): 2075 - 2079. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. L. Krisher, M. Lane, and B. D. Bavister Developmental Competence and Metabolism of Bovine Embryos Cultured in Semi-Defined and Defined Culture Media Biol Reprod, June 1, 1999; 60(6): 1345 - 1352. [Abstract] [Full Text]
|
|
|
|
|
|
D. M. Eberhardt, W. A. Will, and J. D. Godkin Retinol Administration to Superovulated Ewes Improves In Vitro Embryonic Viability Biol Reprod, June 1, 1999; 60(6): 1483 - 1487. [Abstract] [Full Text]
|
|
|
|
|
|
D. N. Wells, P. M. Misica, and H. R. Tervit Production of Cloned Calves Following Nuclear Transfer with Cultured Adult Mural Granulosa Cells Biol Reprod, April 1, 1999; 60(4): 996 - 1005. [Abstract] [Full Text]
|
|
|
|
|
|
J.M. Lim, B.C. Reggio, R.A. Godke, and W. Hansel Development of in-vitro-derived bovine embryos cultured in 5% CO2 in air or in 5% O2, 5% CO2 and 90% N2 Hum. Reprod., February 1, 1999; 14(2): 458 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R.G. Edwards and H. K. Beard Is the success of human IVF more a matter of genetics and evolution than growing blastocysts? Hum. Reprod., January 1, 1999; 14(1): 1 - 6. [Full Text] [PDF]
|
|
|
|
|
|
B. Behr Blastocyst culture and transfer Hum. Reprod., January 1, 1999; 14(1): 5 - 6. [Full Text] [PDF]
|
|
|
|
|
|
I. Spyropoulou, C. Karamalegos, and V. N. Bolton A prospective randomized study comparing the outcome of in-vitro fertilization and embryo transfer following culture of human embryos individually or in groups before embryo transfer on day 2 Hum. Reprod., January 1, 1999; 14(1): 76 - 79. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
