|
|
||||||||
Biology of Reproduction, Vol 50, 543-552, Copyright © 1994 by Society for the Study of Reproduction
ARTICLES |
NA Brown and CL Bethea
Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton 97006.
Rhesus monkey (Macaca mulatta) decidual prolactin cDNA was cloned and sequenced. The first 210 bp of the monkey sequence is not contiguous to the human pituitary prolactin transcription initiation site. Reverse transcription-polymerase chain reaction (RT-PCR) and Southern analysis confirmed that this 5'-extended prolactin transcript is present in monkey decidua but not in pituitary. Isolation and sequencing of a partial genomic monkey prolactin clone verified the nucleotide changes found in the rhesus cDNA. Comparison of the rhesus decidual prolactin cDNA to the human pituitary cDNA revealed more than 97% homology between the two. These data indicate that monkey prolactin mRNA from the decidualized endometrium of pregnancy contains unique 5' sequence not present in pituitary prolactin mRNA, suggesting the use of an alternative start site. Thus it is likely that the decidua uses promoter and regulatory regions different from those reported for pituitary prolactin.
This article has been cited by other articles:
![]() |
E. R. Butelman, S. Rus, D. S. Simpson, A. Wolf, T. E. Prisinzano, and M. J. Kreek The Effects of Herkinorin, the First {micro}-Selective Ligand from a Salvinorin A-Derived Scaffold, in a Neuroendocrine Biomarker Assay in Nonhuman Primates J. Pharmacol. Exp. Ther., October 1, 2008; 327(1): 154 - 160. [Abstract] [Full Text] [PDF] |
||||
![]() |
E. R. Butelman, M. Mandau, K. Tidgewell, T. E. Prisinzano, V. Yuferov, and M. J. Kreek Effects of Salvinorin A, a {kappa}-Opioid Hallucinogen, on a Neuroendocrine Biomarker Assay in Nonhuman Primates with High {kappa}-Receptor Homology to Humans J. Pharmacol. Exp. Ther., January 1, 2007; 320(1): 300 - 306. [Abstract] [Full Text] [PDF] |
||||
![]() |
Y. Pohnke, R. Kempf, and B. Gellersen CCAAT/Enhancer-binding Proteins Are Mediators in the Protein Kinase A-dependent Activation of the Decidual Prolactin Promoter J. Biol. Chem., August 27, 1999; 274(35): 24808 - 24818. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |