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Biology of Reproduction, Vol 50, 581-592, Copyright © 1994 by Society for the Study of Reproduction

ARTICLES

Temporal and regional expression of placental lactogen-II messenger ribonucleic acid and protein in the hamster placenta

MA Jones and RH Renegar
Department of Anatomy and Cell Biology, East Carolina University School of Medicine, Greenville, North Carolina 27858.

The purpose of this investigation was to identify the cell types synthesizing hamster placental lactogen-II (haPL-II) in the chorioallantoic placenta. In addition, temporal changes in haPL-II mRNA expression, in placental mass, and in the proportion of haPL-II- containing cells per unit area were examined. Hamster PL-II mRNA expression was assessed by in situ hybridization and Northern and slot- blot analyses. Cells containing haPL-II protein were identified by immunocytochemical techniques. Placentae were recovered in the morning on Days 6, 8, 10, 12, and 14 of gestation and in the afternoon on Day 15. A single 1-kb haPL-II transcript was first detected on Day 10 of gestation. Hamster PL-II message increased to Day 14 and remained elevated on Day 15. Giant trophoblast cells (GTC) located in the trophospongium were the major source of haPL-II mRNA and protein, although GTC along the periphery of the placenta and in the labyrinth expressed haPL-II as well. Placental weight, trophospongium portion of placental weight, and number of haPL-II-immunostained GTC per unit of placental area increased between Days 12 and 14 and remained elevated to Day 15. These observations indicate that an increased number of haPL- II-producing GTC is a major factor contributing to increased maternal haPL-II serum levels during pregnancy.


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Copyright © 1994 by the Society for the Study of Reproduction.