Biology of Reproduction, Vol 50, 664-668, Copyright © 1994 by Society for the Study of Reproduction

ARTICLES

Transgenic cattle resulting from biopsied embryos: expression of c-ski in a transgenic calf

RA Bowen, ML Reed, A Schnieke, GE Seidel Jr, A Stacey, WK Thomas and O Kajikawa
Animal Reproduction and Biotechnology Laboratory, Colorado State University, Fort Collins 80523.

Producing transgenic cattle by microinjection of DNA into pronuclei has been inefficient and costly, in large part because of the cost of maintaining numerous nontransgenic pregnancies to term. We designed a system for early identification of transgenic embryos in which biopsies of embryos were assayed by polymerase chain reaction for presence of the transgene before embryo transfer. A total of 2555 embryos were microinjected with one of two DNA constructs. Of the 533 embryos biopsied, 112 were judged to be potentially transgenic and were transferred nonsurgically to recipients, resulting in production of 29 putative transgenic fetuses. One fetus and one calf (7% of offspring) were subsequently shown to be definitively transgenic. The calf was transgenic for a chicken c-ski cDNA, and several months after birth developed dramatic muscular hypertrophy followed by muscle degeneration. This phenotype was associated with expression of high levels of mRNA from the transgene.

This article has been cited by other articles:

				M. D. Glover and G. E. Seidel Jr Increased Messenger RNA for Allograft Inflammatory Factor-1, LERK-5, and a Novel Gene in 17.5-Day Relative to 15.5-Day Bovine Embryos Biol Reprod, September 1, 2003; 69(3): 1002 - 1012. [Abstract] [Full Text] [PDF]

				V. Bordignon, R. Keyston, A. Lazaris, A. S. Bilodeau, J. H.F. Pontes, D. Arnold, G. Fecteau, C. Keefer, and L. C. Smith Transgene Expression of Green Fluorescent Protein and Germ Line Transmission in Cloned Calves Derived from In Vitro-Transfected Somatic Cells Biol Reprod, June 1, 2003; 68(6): 2013 - 2023. [Abstract] [Full Text] [PDF]

				S.-H. Chen, T. D. Vaught, J. A. Monahan, J. Boone, E. Emslie, P. M. Jobst, A. E. Lamborn, A. Schnieke, L. Robertson, A. Colman, et al. Efficient Production of Transgenic Cloned Calves Using Preimplantation Screening Biol Reprod, November 1, 2002; 67(5): 1488 - 1492. [Abstract] [Full Text] [PDF]

				S. B. P. Charge, A. S. Brack, and S. M. Hughes Aging-related satellite cell differentiation defect occurs prematurely after Ski-induced muscle hypertrophy Am J Physiol Cell Physiol, October 1, 2002; 283(4): C1228 - C1241. [Abstract] [Full Text] [PDF]

				A. E. Schnieke, A. J. Kind, W. A. Ritchie, K. Mycock, A. R. Scott, M. Ritchie, I. Wilmut, A. Colman, and K. H. Campbell Human Factor IX Transgenic Sheep Produced by Transfer of Nuclei from Transfected Fetal Fibroblasts Science, December 19, 1997; 278(5346): 2130 - 2133. [Abstract] [Full Text]