Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sawada, H.
Right arrow Articles by Yazama, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sawada, H.
Right arrow Articles by Yazama, F.
Agricola
Right arrow Articles by Sawada, H.
Right arrow Articles by Yazama, F.

Biology of Reproduction, Vol 50, 702-710, Copyright © 1994 by Society for the Study of Reproduction

ARTICLES

Type VI collagen in the rat testis: monoclonal antibody, isolation, and localization during development

H Sawada and F Yazama
Department of Anatomy, Yokohama City University, School of Medicine, Kanagawa, Japan.

To investigate the components of the seminiferous tubule extracellular matrix (ST-ECM), monoclonal antibodies were raised against ST-ECM. One of these (15B6) recognized the nonreduced form of type VI collagen. With this antibody as a probe, type VI collagen was isolated from an SDS-urea extract of ST-ECM. It took a tetrameric form, and upon reduction dissociated into a major 140-kDa band and two bands at 190 and 210 kDa. Developmental changes in the distribution of type VI collagen were investigated by immunofluorescence microscopy. In 16-day rat embryos when myoid cells began to differentiate, type VI, collagen was seen in the urogenital mesentery, and it had begun to surround the gonad and, in some embryos, the seminiferous cords. In newborn rats, it was found throughout the extracellular spaces between the cords, being localized in the interstitium. In adults, however, it was confined to two parallel lines around the seminiferous tubules and to filamentous structures in the adventitia of vascular systems. Immunoelectron microscopy showed that this collagen was distributed in the interstitium independently from collagenous fibrils, but was excluded from the basal lamina of Sertoli cells. These observations indicate that type VI collagen is a major component of the ST-ECM and that it may be important in the organization of the matrix and in the differentiation of peritubular cells.


This article has been cited by other articles:


Home page
 J AndrolHome page
I. Fenic, H. M. Hossain, V. Sonnack, S. Tchatalbachev, F. Thierer, J. Trapp, K. Failing, K. S. Edler, M. Bergmann, M. Jung, et al.
In Vivo Application of Histone Deacetylase Inhibitor Trichostatin-A Impairs Murine Male Meiosis
J Androl, March 1, 2008; 29(2): 172 - 185.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. Lustig, B. Denduchis, R. Ponzio, M. Lauzon, and R.-M. Pelletier
Passive Immunization with Anti-Laminin Immunoglobulin G Modifies the Integrity of the Seminiferous Epithelium and Induces Arrest of Spermatogenesis in the Guinea Pig
Biol Reprod, June 1, 2000; 62(6): 1505 - 1514.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the Society for the Study of Reproduction.