Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Watson, A. J.
Right arrow Articles by Seamark, R. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Watson, A. J.
Right arrow Articles by Seamark, R. F.
Agricola
Right arrow Articles by Watson, A. J.
Right arrow Articles by Seamark, R. F.

Biology of Reproduction, Vol 50, 725-733, Copyright © 1994 by Society for the Study of Reproduction

ARTICLES

A growth factor phenotype map for ovine preimplantation development

AJ Watson, PH Watson, M Arcellana-Panlilio, D Warnes, SK Walker, GA Schultz, DT Armstrong and RF Seamark
Department of Obstetrics and Gynaecology, University of Western Ontario, London, Canada.

The reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the patterns of expression for several growth factor ligand and receptor genes during ovine preimplantation development. Transcripts for insulin-like growth factor (IGF)-I, IGF-II, and the receptors for insulin and IGF-I were detected throughout ovine preimplantation development from the 1-cell to the blastocyst stage. Transforming growth factor alpha (TGF alpha) transcripts were also detected throughout ovine preimplantation development. The mRNAs encoding basic fibroblast growth factor (bFGF) were detected in all stages of the ovine preimplantation embryo, although the relative abundance of this transcript consistently decreased from the 1-cell to the blastocyst stage, suggesting that it may represent a maternal transcript in early sheep embryos. Transcripts encoding ovine trophoblast protein (oTP) were detected only within blastocyst-stage embryos. Primary ovine oviduct cell cultures express the transcripts for IGF-II, IGF-I, TGF alpha, bFGF, TGF beta 1, and the receptors for insulin and IGF-I, suggesting that paracrine growth factor circuits may exist between the oviduct epithelium and the early ovine embryo. Transcripts for insulin, epidermal growth factor (EGF), and nerve growth factor (NGF) were not detected in any stage of the ovine preimplantation embryo or within the oviduct cell preparations. The expression of growth factor transcripts very early in mammalian development would predict that these molecules fulfil a necessary role(s) in supporting the progression of early embryos through the preimplantation interval. Our future efforts will be directed to understanding the nature of these putative regulatory pathways.


This article has been cited by other articles:


Home page
 ReproductionHome page
A A Fouladi-Nashta and K H S Campbell
Dissociation of oocyte nuclear and cytoplasmic maturation by the addition of insulin in cultured bovine antral follicles.
Reproduction, March 1, 2006; 131(3): 449 - 460.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M.L. Keller, A.J. Roberts, and G.E. Seidel Jr.
Characterization of Insulin-Like Growth Factor-Binding Proteins in the Uterus and Conceptus during Early Conceptus Elongation in Cattle
Biol Reprod, July 1, 1998; 59(3): 632 - 642.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
C. Chia, R. Winston, and A. Handyside
EGF, TGF-alpha and EGFR expression in human preimplantation embryos
Development, January 2, 1995; 121(2): 299 - 307.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the Society for the Study of Reproduction.