| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology of Reproduction, Vol 51, 116-124, Copyright © 1994 by Society for the Study of Reproduction
ARTICLES |
MP McGuinness, CC Linder, CR Morales, LL Heckert, J Pikus and MD Griswold
Department of Biochemistry and Biophysics, Washington State University, Pullman 99164-4460.
Recently, a mouse Sertoli cell line (MSC-1) was established from transgenic mice carrying a fusion gene composed of human Mullerian inhibitory substance transcriptional regulatory sequences linked to the SV40 T-antigen gene. This cell line contained a morphologically heterogeneous population of cells that expressed characteristic Sertoli cell mRNAs such as transferrin, the beta-subunit for inhibin, and sulfated glycoprotein-2 (SGP-2). In this study, we compared various characteristics of MSC-1 cells to primary cultures of Sertoli cells from immature rats or adult mice. Our observations indicated that: 1) These cells were ultrastructurally similar to mouse Sertoli cells in culture; 2) MSC-1 cells expressed mRNA for androgen-binding protein (ABP) and SGP-1, but not the receptor for FSH; 3) The expression of SGP- 2 mRNA and secretion of SGP-2 increased approximately 2-fold when cells were cultured at 41 degrees C, the nonpermissive temperature for the SV40 virus, while SGP-1 and transferrin mRNA levels and secretion were unaffected; 4) Proliferation of these cells was serum-dose dependent and temperature dependent and could be inhibited by incubating MSC-1 cells at 41 degrees C; 5) Proliferation was also significantly reduced after cells were incubated in the presence of dibutyryl cAMP (dbcAMP) for 6 days; 6) Fifteen subcultures produced from single MSC-1 cells displayed similar levels of mRNA expression for transferrin or SGP-1. Together these data indicate that the MSC-1 cell line is composed of a single cell type displaying numerous characteristics of Sertoli cells. This cell line may provide a unique source of tissue for studying various aspects of Sertoli cell behavior.
This article has been cited by other articles:
|
|
 |
|
  B. P. Hermann, K. Hornbaker, D. A. Rice, M. Sawadogo, and L. L. Heckert In Vivo Regulation of Follicle-Stimulating Hormone Receptor by the Transcription Factors Upstream Stimulatory Factor 1 and Upstream Stimulatory Factor 2 Is Cell Specific Endocrinology, October 1, 2008; 149(10): 5297 - 5306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R.-S. Fujino, K. Tanaka, M. Morimatsu, K. Tamura, H. Kogo, and T. Hara Spermatogonial Cell-Mediated Activation of an I{kappa}B{zeta}-Independent Nuclear Factor-{kappa}B Pathway in Sertoli Cells Induces Transcription of the Lipocalin-2 Gene Mol. Endocrinol., April 1, 2006; 20(4): 904 - 915. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. M. Robert, L. J. Martin, and J. J. Tremblay The Orphan Nuclear Receptor NR4A1 Regulates Insulin-Like 3 Gene Transcription in Leydig Cells Biol Reprod, February 1, 2006; 74(2): 322 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. F. Sneddon, N. Walther, and P. T. K. Saunders Expression of Androgen and Estrogen Receptors in Sertoli Cells: Studies Using the Mouse SK11 Cell Line Endocrinology, December 1, 2005; 146(12): 5304 - 5312. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Robertson, G. U. Schuster, K. R. Steffensen, O. Hovatta, S. Meaney, K. Hultenby, L. C. Johansson, K. Svechnikov, O. Soder, and J.-A. Gustafsson The Liver X Receptor-{beta} Is Essential for Maintaining Cholesterol Homeostasis in the Testis Endocrinology, June 1, 2005; 146(6): 2519 - 2530. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Chaudhary, I. Sadler-Riggleman, J. M. Ague, and M. K. Skinner The Helix-Loop-Helix Inhibitor of Differentiation (ID) Proteins Induce Post-Mitotic Terminally Differentiated Sertoli Cells to Re-Enter the Cell Cycle and Proliferate Biol Reprod, May 1, 2005; 72(5): 1205 - 1217. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hoang, I. S. Fenne, C. Cook, B. Borud, M. Bakke, E. A. Lien, and G. Mellgren cAMP-dependent Protein Kinase Regulates Ubiquitin-Proteasome-mediated Degradation and Subcellular Localization of the Nuclear Receptor Coactivator GRIP1 J. Biol. Chem., November 19, 2004; 279(47): 49120 - 49130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Scobey, C. A. Fix, and W. H. Walker The Id2 Transcriptional Repressor Is Induced by Follicle-stimulating Hormone and cAMP J. Biol. Chem., April 16, 2004; 279(16): 16064 - 16070. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Krishnamurthy, H. Kishi, M. Shi, C. Galet, R. S. Bhaskaran, T. Hirakawa, and M. Ascoli Postendocytotic Trafficking of the Follicle-Stimulating Hormone (FSH)-FSH Receptor Complex Mol. Endocrinol., November 1, 2003; 17(11): 2162 - 2176. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Tirado, E. D. Martinez, O. C. Rodriguez, M. Danielsen, D. M. Selva, J. Reventos, F. Munell, and C. A. Suarez-Quian Methoxyacetic Acid Disregulation of Androgen Receptor and Androgen-Binding Protein Expression in Adult Rat Testis Biol Reprod, April 1, 2003; 68(4): 1437 - 1446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Dufour, M.-N. Vo, N. Bhattacharya, J. Okita, R. Okita, and K. H. Kim Peroxisome Proliferators Disrupt Retinoic Acid Receptor Alpha Signaling in the Testis Biol Reprod, April 1, 2003; 68(4): 1215 - 1224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Her, X. Wu, M. D. Griswold, and F. Zhou Human MutS Homologue MSH4 Physically Interacts with von Hippel-Lindau Tumor Suppressor-binding Protein 1 Cancer Res., February 15, 2003; 63(4): 865 - 872. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Hofmann, K. S. Van Der Wee, J. L. Dargart, G. Dirami, L. Dettin, and M. Dym Establishment and Characterization of Neonatal Mouse Sertoli Cell Lines J Androl, January 1, 2003; 24(1): 120 - 130. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. W. Braun, M.-N. Vo, and K. H. Kim Positive Regulation of Retinoic Acid Receptor Alpha by Protein Kinase C and Mitogen-Activated Protein Kinase in Sertoli Cells Biol Reprod, July 1, 2002; 67(1): 29 - 37. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. L. Heckert and M. D. Griswold The Expression of the Follicle-stimulating Hormone Receptor in Spermatogenesis Recent Prog. Horm. Res., January 1, 2002; 57(1): 129 - 148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. k. Chen and L. L. Heckert Dmrt1 Expression Is Regulated by Follicle-Stimulating Hormone and Phorbol Esters in Postnatal Sertoli Cells Endocrinology, March 1, 2001; 142(3): 1167 - 1178. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Griswold and J.-S. Kim Site-Specific Methylation of the Promoter Alters Deoxyribonucleic Acid-Protein Interactions and Prevents Follicle-Stimulating Hormone Receptor Gene Transcription Biol Reprod, February 1, 2001; 64(2): 602 - 610. [Abstract] [Full Text]
|
|
|
|
|
|
Z.-M. Feng, A. Z. Wu, Z. Zhang, and C.-L. C. Chen GATA-1 and GATA-4 Transactivate Inhibin/Activin {beta}-B-Subunit Gene Transcription in Testicular Cells Mol. Endocrinol., November 1, 2000; 14(11): 1820 - 1835. [Abstract] [Full Text]
|
|
|
|
|
|
L. L. Heckert, M. Sawadogo, M. A. F. Daggett, and J. k. Chen The USF Proteins Regulate Transcription of the Follicle-Stimulating Hormone Receptor but Are Insufficient for Cell- Specific Expression Mol. Endocrinol., November 1, 2000; 14(11): 1836 - 1848. [Abstract] [Full Text]
|
|
|
|
|
|
E. S. Lymar, A. M. Clark, R. Reeves, and M. D. Griswold Clusterin Gene in Rat Sertoli Cells Is Regulated by a Core-Enhancer Element Biol Reprod, November 1, 2000; 63(5): 1341 - 1351. [Abstract] [Full Text]
|
|
|
|
|
|
K. W. Braun, W. A. Tribley, M. D. Griswold, and K. H. Kim Follicle-stimulating Hormone Inhibits All-trans-retinoic Acid-induced Retinoic Acid Receptor alpha Nuclear Localization and Transcriptional Activation in Mouse Sertoli Cell Lines J. Biol. Chem., February 11, 2000; 275(6): 4145 - 4151. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. d. F. M. Lazari, X. Liu, K. Nakamura, J. L. Benovic, and M. Ascoli Role of G Protein-Coupled Receptor Kinases on the Agonist-Induced Phosphorylation and Internalization of the Follitropin Receptor Mol. Endocrinol., June 1, 1999; 13(6): 866 - 878. [Abstract] [Full Text]
|
|
|
|
|
|
F. Delfino and W. H. Walker Stage-Specific Nuclear Expression of NF-{kappa}B in Mammalian Testis Mol. Endocrinol., November 1, 1998; 12(11): 1696 - 1707. [Abstract] [Full Text]
|
|
|
|
|
|
D. A. Fenstermacher and D. R. Joseph DNA Sequences and Their Binding Proteins Required for Sertoli Cell-Specific Transcription of the Rat Androgen-Binding Protein Gene Mol. Endocrinol., August 1, 1997; 11(9): 1387 - 1400. [Abstract] [Full Text]
|
|
|
|
|
|
T. L. Goetz, T. L. Lloyd, and M. D. Griswold Role of E Box and Initiator Region in the Expression of the Rat Follicle-stimulating Hormone Receptor J. Biol. Chem., December 27, 1996; 271(52): 33317 - 33324. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
