Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Juengel, J. L.
Right arrow Articles by Niswender, G. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Juengel, J. L.
Right arrow Articles by Niswender, G. D.
Agricola
Right arrow Articles by Juengel, J. L.
Right arrow Articles by Niswender, G. D.

Biology of Reproduction, Vol 51, 380-384, Copyright © 1994 by Society for the Study of Reproduction

ARTICLES

Steady-state concentrations of messenger ribonucleic acid encoding cytochrome P450 side-chain cleavage and 3 beta-hydroxysteroid dehydrogenase/delta 5,delta 4 isomerase in ovine corpora lutea during the estrous cycle

JL Juengel, MK Guy, TR Tandeski, WJ McGuire and GD Niswender
Department of Physiology, Colorado State University, Fort Collins 80523.

The objective of these experiments was to determine the pattern of mRNA expression for cytochrome P450 side-chain cleavage (P450scc) and 3 beta- hydroxysteroid dehydrogenase/delta 5,delta 4 isomerase (3 beta-HSD) during luteinization of the follicle and in ovine luteal tissue on Days 3, 6, 9, 12, and 15 of the estrous cycle. Mean concentration of mRNA for P450scc was not different in follicles collected 4 or 24 h after the onset of estrus but increased (p < 0.05) 3-fold by 48 h (corpus hemorrhagicum). With the methods used, mRNA for 3 beta-HSD was not detected until after ovulation and formation of the corpus hemorrhagicum (48 h after onset of estrus). In luteal tissue, mean concentration of mRNA for P450scc increased from Days 3 to 9 (p < 0.05) and had decreased (p < 0.05) by Day 15. Mean concentration of mRNA for P450scc was higher (p < 0.05) in small luteal cells on Day 9 than on Day 15, with values on Days 6 and 12 being intermediate. In large luteal cells, mean concentrations of P450scc mRNA increased (p < 0.05) between Days 6 and 12 and then decreased (p < 0.05) on Day 15. Mean concentration of mRNA for 3 beta-HSD was not different (p = 0.33) in luteal tissue on any day examined. In small luteal cells, mean concentrations of mRNA for 3 beta-HSD decreased between Days 6 and 15 (p < 0.05) while in large luteal cells, mean concentrations decreased (p < 0.05) between Days 12 and 15.(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
 Endocr. Rev.Home page
J. Simard, M.-L. Ricketts, S. Gingras, P. Soucy, F. A. Feltus, and M. H. Melner
Molecular Biology of the 3{beta}-Hydroxysteroid Dehydrogenase/{Delta}5-{Delta}4 Isomerase Gene Family
Endocr. Rev., June 1, 2005; 26(4): 525 - 582.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
R. S. McRae, H. M. Johnston, M. Mihm, and P. J. O'Shaughnessy
Changes in Mouse Granulosa Cell Gene Expression during Early Luteinization
Endocrinology, January 1, 2005; 146(1): 309 - 317.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. A. Logan, J. L. Juengel, and K. P. McNatty
Onset of Steroidogenic Enzyme Gene Expression During Ovarian Follicular Development in Sheep
Biol Reprod, April 1, 2002; 66(4): 906 - 916.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. D. Quirke, J. L. Juengel, D. J. Tisdall, S. Lun, D. A. Heath, and K. P. McNatty
Ontogeny of Steroidogenesis in the Fetal Sheep Gonad
Biol Reprod, July 1, 2001; 65(1): 216 - 228.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. Boerboom and J. Sirois
Equine P450 Cholesterol Side-Chain Cleavage and 3{beta}-Hydroxysteroid Dehydrogenase/{{Delta}}5-{{Delta}}4 Isomerase: Molecular Cloning and Regulation of Their Messenger Ribonucleic Acids in Equine Follicles During the Ovulatory Process
Biol Reprod, January 1, 2001; 64(1): 206 - 215.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
J.L. Juengel, J.D. Haworth, M.K. Rollyson, P.J. Silva, H.R. Sawyer, and G.D. Niswender
Effect of Dose of Prostaglandin F2{alpha} on Steroidogenic Components and Oligonucleosomes in Ovine Luteal Tissue
Biol Reprod, April 1, 2000; 62(4): 1047 - 1051.
[Abstract] [Full Text]

Home page
 Physiol. Rev.Home page
G. D. Niswender, J. L. Juengel, P. J. Silva, M. K. Rollyson, and E. W. McIntush
Mechanisms Controlling the Function and Life Span of the Corpus Luteum
Physiol Rev, January 1, 2000; 80(1): 1 - 29.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
N. Pescador, D. M. Stocco, and B. D. Murphy
Growth Factor Modulation of Steroidogenic Acute Regulatory Protein and Luteinization in the Pig Ovary
Biol Reprod, June 1, 1999; 60(6): 1453 - 1461.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
R. Mamluk, Y. Greber, and R. Meidan
Hormonal Regulation of Messenger Ribonucleic Acid Expression for Steroidogenic Factor-1, Steroidogenic Acute Regulatory Protein, and Cytochrome P450 Side-Chain Cleavage in Bovine Luteal Cells
Biol Reprod, March 1, 1999; 60(3): 628 - 634.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
A. M. Turzillo, T. E. Nolan, and T. M. Nett
Regulation of Gonadotropin-Releasing Hormone (GnRH) Receptor Gene Expression in Sheep: Interaction of GnRH and Estradiol
Endocrinology, December 1, 1998; 139(12): 4890 - 4894.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1994 by the Society for the Study of Reproduction.