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Biology of Reproduction, Vol 52, 753-758, Copyright © 1995 by Society for the Study of Reproduction

ARTICLES

Parthenogenetic activation of porcine oocytes with guanosine-5'-O-(3'- thiotriphosphate)

Z Machaty, MA Mayes and RS Prather
Department of Animal Sciences, University of Missouri-Columbia 65211, USA.

A possible mechanism by which sperm activate oocytes may involve a G protein-mediated signal-transducing pathway. Artificial stimulation of G proteins leads to events very similar to those at fertilization in oocytes of several species. Here we report the effects of activation of a G protein with guanosine-5'-O-(3'-thiotriphosphate) (GTP-gamma-S), a hydrolysis-resistant analog of guanosine triphosphate (GTP), in porcine oocytes. Mature oocytes were injected with 1 mM GTP-gamma-S. In response to injection, oocytes underwent changes normally occurring at fertilization, i.e. cortical granule exocytosis, changes in the protein synthetic profile, pronuclear formation, and development to the blastocyst stage. Guanosine-5'-O-(2-thiodiphosphate) (GDP-beta-S; a metabolically stable analog of guanosine diphosphate [GDP] that inactivates the G protein) and the carrier medium (CM; medium in which the injected compounds were dissolved) were significantly less effective in oocyte activation. It is suggested that porcine oocytes contain the components of a G protein-mediated signaling pathway that might function in oocyte activation at fertilization.


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J. Ito, M. Shimada, and T. Terada
Effect of Protein Kinase C Activator on Mitogen-Activated Protein Kinase and p34cdc2 Kinase Activity During Parthenogenetic Activation of Porcine Oocytes by Calcium Ionophore
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J.-H. Kim, Z. Macháty, R. A. Cabot, Y.-M. Han, H.-J. Do, and R. S. Prather
Development of Pig Oocytes Activated by Stimulation of an Exogenous G Protein-Coupled Receptor
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