Biology of Reproduction, Vol 52, 848-854, Copyright © 1995 by Society for the Study of Reproduction

ARTICLES

Secretion of bovine uterine proteins in response to type I interferons

KA Naivar, SK Ward, KJ Austin, DW Moore and TR Hansen
Department of Animal Science, University of Wyoming Laramie 82071, USA.

Bovine interferon-tau (bIFN-tau) is secreted by the developing conceptus and initiates antiluteolytic events by interacting with uterine membrane receptors. We have identified three endometrial proteins (approximately 8, 16, and 28 kDa; P8, P16, and P28; respectively) that are secreted in response to recombinant (r) bIFN- tau. The objective of this study was to determine whether or not secretion of these proteins was a unique response to IFN-tau during early pregnancy. Three experiments were designed to examine secretion of endometrial proteins as a function of time in culture (0, 3, 6, 12, 18, 24 h), stage of the estrous cycle and pregnancy (Days 15, 18, 0/21), and dose of Type I IFN (0, 0.5, 5, and 25 nM; rbIFN-tau, rbIFN- alpha, and roIFN-tau). Endometrium was cultured for times specified with L-[3H]leusine to generate radiolabeled proteins. Secreted proteins were quantitated by using one-dimensional (1D)-PAGE, fluorography, and densitometry. Secretion of P8, P16, and P28 increased over time (p < 0.0001) in culture and in response to 25 nM rbIFN-tau (p < 0.05). Secretion of P8 in response to rbIFN-tau was higher (p < 0.05). Secretion of P8 in response to rbIFN-tau was higher (p < 0.0005) in endometrium collected from pregnant than nonpregnant heifers, but did not differ across the days examined. Although secretion of P8 was higher (p < 0.0001) in the presence than in the absence of rbIFN-tau, it was not affected by rbIFN-alpha.(ABSTRACT TRUNCATED AT 250 WORDS)

This article has been cited by other articles:

				A. Guzeloglu, P. Subramaniam, F. Michel, and W. W. Thatcher Interferon-{tau} Induces Degradation of Prostaglandin H Synthase-2 Messenger RNA in Bovine Endometrial Cells Through a Transcription-Dependent Mechanism Biol Reprod, July 1, 2004; 71(1): 170 - 176. [Abstract] [Full Text] [PDF]

				C. Bebington, F.J. Doherty, and S.D. Fleming Ubiquitin cross-reactive protein gene expression is increased in decidualized endometrial stromal cells at the initiation of pregnancy Mol. Hum. Reprod., October 1, 1999; 5(10): 966 - 972. [Abstract] [Full Text] [PDF]

				G. A. Johnson, T. E. Spencer, T. R. Hansen, K. J. Austin, R. C. Burghardt, and F. W. Bazer Expression of the Interferon Tau Inducible Ubiquitin Cross-Reactive Protein in the Ovine Uterus Biol Reprod, July 1, 1999; 61(1): 312 - 318. [Abstract] [Full Text]

				C. Bebington, S.C. Bell, F.J. Doherty, A.T. Fazleabas, and S.D. Fleming Localization of Ubiquitin and Ubiquitin Cross-Reactive Protein in Human and Baboon Endometrium and Decidua during the Menstrual Cycle andEarly Pregnancy Biol Reprod, April 1, 1999; 60(4): 920 - 928. [Abstract] [Full Text]

				K. L. Staggs, K. J. Austin, G. A. Johnson, M. G. Teixeira, C. T. Talbott, V. A. Dooley, and T. R. Hansen Complex Induction of Bovine Uterine Proteins by Interferon-Tau Biol Reprod, August 1, 1998; 59(2): 293 - 297. [Abstract] [Full Text]