Biology of Reproduction, Vol 52, 939-946, Copyright © 1995 by Society for the Study of Reproduction

ARTICLES

Progesterone secretion and proliferation in cultured rabbit granulosa cells under conditions of beta-D-xyloside-induced inhibition of proteoglycan synthesis

A Benhaim, C Feral, M Langris, J Bocquet and P Leymarie
Laboratoire de Biochimie, ER 90 CNRS-IBBA, CHU Cote de Nacre, Universite de Caen, France.

Proteoglycans present in follicular fluid are synthesized by granulosa cells under gonadotropin control. An inhibitor of proteoglycan synthesis, p-nitrophenyl-beta-D-xyloside (beta-D-xyloside) was used as a probe to study rabbit granulosa cell steroidogenesis and proliferation under abrogated proteoglycan synthesis. Granulosa cells isolated from rabbit preovulatory follicles were cultured 24 h in Minimum Essential Medium plus 2.5% fetal calf serum in the presence or absence of beta-D-xyloside and were then treated with FSH or dibutyryl cAMP (db-cAMP) alone or in combination with beta-D-xyloside for a further 24 h. The exposure for 48 h of granulosa cells to 1 mM beta-D- xyloside in the absence or presence of FSH inhibited proteoglycan synthesis and increased the amount of glycosaminoglycans (GAG). FSH- stimulated progesterone production was significantly correlated only with proteoglycan synthesis and not with GAG production. The addition of various concentrations of beta-D-xyloside (0.1-4 mM) for 48 h to granulosa cells induced a dose-dependent inhibition of FSH-stimulated progesterone secretion and [3H]thymidine incorporation into DNA. beta-D- Xyloside concentrations lower than 1 mM induced an inhibition of FSH- stimulated progesterone secretion but had no significant effect on FSH- induced proliferation. One millimolar beta-D-xyloside did not modify basal progesterone production, but in the presence of various doses (0.1-2.5 ng/ml) of FSH or hCG (0.1-1 IU/ml) it exerted a significant inhibitory effect on steroid secretion. Fifty percent inhibition was obtained for doses of FSH above 0.5 ng/ml.(ABSTRACT TRUNCATED AT 250 WORDS)

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