Biology of Reproduction, Vol 53, 126-132, Copyright © 1995 by Society for the Study of Reproduction

ARTICLES

Interleukin-1 alpha regulates prostaglandin production and cyclooxygenase activity in sensitized rat endometrial stromal cells in vitro

BM Bany and TG Kennedy
Department of Physiology, University of Western Ontario, Canada.

This study was an examination of the effect of interleukin-1 alpha (IL- 1 alpha) on prostaglandin (PG) production and cyclooxygenase (COX) activity in endometrial stromal cells isolated from uteri of ovariectomized rats sensitized for the decidual cell reaction. Treatment with IL-1 alpha (20 ng/ml) for 24 h caused approximately 13-, 2-, and 2-fold increases in PGE2, PGF2 alpha, and 13, 14-dihydro-15- keto-PGF2 alpha (PGFM) accumulation, respectively, in the medium. The IL-1 alpha-induced increase in PG accumulation was accompanied by a significant (6-fold) increase in COX activity in the cells. IL-1 alpha- induced increases in PGE2 accumulation and COX activity were greatly reduced or prevented by cycloheximide (CHX), actinomycin D, and dexamethasone (DEX). In addition to IL-1 alpha, epidermal growth factor (EGF, 40 ng/ml) also caused a significant increase in PGE2 accumulation in the medium and COX activity in the cells. The combined effects of IL- 1 alpha and EGF on PGE2 accumulation and COX activity were additive. The results of this study provide evidence that IL-1 alpha stimulates PG production in endometrial stromal cells from uteri sensitized for the decidual cell reaction through a mechanism that involves an increase in COX activity.

This article has been cited by other articles:

				T. G Kennedy, C. Gillio-Meina, and S. H. Phang Prostaglandins and the initiation of blastocyst implantation and decidualization Reproduction, November 1, 2007; 134(5): 635 - 643. [Abstract] [Full Text] [PDF]

				S. J Kimber Leukaemia inhibitory factor in implantation and uterine biology Reproduction, August 1, 2005; 130(2): 131 - 145. [Abstract] [Full Text] [PDF]

				K. Deb, M. M Chaturvedi, and Y. K Jaiswal A 'minimum dose' of lipopolysaccharide required for implantation failure: assessment of its effect on the maternal reproductive organs and interleukin-1{alpha} expression in the mouse Reproduction, July 1, 2004; 128(1): 87 - 97. [Abstract] [Full Text] [PDF]

				L. Marions and K. G. Danielsson Expression of cyclo-oxygenase in human endometrium during the implantation period Mol. Hum. Reprod., October 1, 1999; 5(10): 961 - 965. [Abstract] [Full Text] [PDF]

				B. M. Bany, X. Zhang, and T. G. Kennedy Effects of Epidermal Growth Factor and Interleukin-1{alpha} on Plasminogen Activator Secretion and Decidualization in Rat Endometrial Stromal Cells Biol Reprod, July 1, 1998; 59(1): 131 - 135. [Abstract] [Full Text]

				J.-C. Huang, D.-Y. Liu, S. Yadollahi, K. K. Wu, and M. Y. Dawood Interleukin-1{beta} Induces Cyclooxygenase-2 Gene Expression in Cultured Endometrial Stromal Cells J. Clin. Endocrinol. Metab., February 1, 1998; 83(2): 538 - 541. [Abstract] [Full Text]