Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Vizziano, D.
Right arrow Articles by Loir, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Vizziano, D.
Right arrow Articles by Loir, M.
Agricola
Right arrow Articles by Vizziano, D.
Right arrow Articles by Loir, M.

Biology of Reproduction, Vol 54, 1-7, Copyright © 1996 by Society for the Study of Reproduction

ARTICLES

20 beta-hydroxysteroid dehydrogenase activity in nonflagellated germ cells of rainbow trout testis

D Vizziano, A Fostier, F Le Gac and M Loir
Laboratoire de Physiologie des Poissons, Institut National de la Recherche Agronomique, Rennes, France.

Nonflagellated germ cells were isolated from rainbow trout testis to determine their ability to synthesize 17,20 beta-dihydroxy-4-pregnen-3- one (17,20 beta OHP), a progestin involved in the control of the release of sperm. Germ cells were obtained by enzymatic dissociation (collagenase; 3 mg.ml-1, 4.5 h, 12 degrees C) from testes that were immature and at the beginning of spermatogenesis. Somatic cells were eliminated by adhesion to the culture plates. Dose-related amounts of 17,20 beta OHP were measured by RIA in culture media of germ cells incubated with increasing dosages of 17-hydroxyprogesterone (17OHP; 0.05-10 micrograms.ml-1) for 20 h at 12 degrees C. Furthermore, 3H- 17,20 beta OHP was identified by chromatography and co-crystallization with a reference in incubating cells provided by 3H-17OHP (2.5 and 4 h, 12 degrees C). Other metabolites were detected but not identified. 11- Ketotestosterone (11KT) was either nondetectable by RIA in control cultures or, when detected, was found at very low levels. In no case was 11KT stimulated by addition of 17OHP or gonadotropin II (GtH II; 400 ng.ml-1); this indicated the absence of contamination by Leydig cells. Thus, to our knowledge, this report demonstrates steroidogenic activities in nonflagellated germ cells of fish testis for the first time. 20 beta-Hydroxysteroid dehydrogenase (20 beta HSD) activity was identified, showing that germ cells are able to synthesize 17,20 beta OHP at an early stage in rainbow trout testis.


This article has been cited by other articles:


Home page
 J EndocrinolHome page
M Kusakabe, I Nakamura, J Evans, P Swanson, and G Young
Changes in mRNAs encoding steroidogenic acute regulatory protein, steroidogenic enzymes and receptors for gonadotropins during spermatogenesis in rainbow trout testes.
J. Endocrinol., June 1, 2006; 189(3): 541 - 554.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. A. Amer, T. Miura, C. Miura, and K. Yamauchi
Involvement of Sex Steroid Hormones in the Early Stages of Spermatogenesis in Japanese Huchen (Hucho perryi )
Biol Reprod, October 1, 2001; 65(4): 1057 - 1066.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1996 by the Society for the Study of Reproduction.