| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology of Reproduction, Vol 54, 183-196, Copyright © 1996 by Society for the Study of Reproduction
ARTICLES |
SA Robertson, G Mayrhofer and RF Seamark
Department of Obstetrics and Gynaecology, University of Adelaide, South Australia.
Uterine epithelial cells have been shown by in vitro studies to be a potent source of the inflammatory cytokine granulocyte-macrophage colony-stimulating factor (GM-CSF), and the luminal and glandular epithelium has been confirmed as the predominant site of GM-CSF expression in the intact endometrium by in situ hybridization. To examine the role of ovarian steroid hormones in GM-CSF synthesis, GM- CSF bioactivity has been measured in the supernatants of short-term primary cultures of endometrial cells prepared from mice in which steroid levels were perturbed by ovariectomy and steroid replacement or by steroid antagonists. GM-CSF production was found to fluctuate in cells harvested at different times during the estrous cycle, peaking at estrus. Endometrial cells derived from ovariectomized mice produced 25- fold less GM-CSF than did cells from estrous mice, and production was increased if ovariectomized mice were pretreated with estrogen, but not progesterone, 3 h or more before harvest. This estrogen-induced increase was inhibited by coadministration of progesterone or by induction of a decidual response and was blocked by the estrogen antagonist ZK 119,010. By contrast, pretreatment of mice with the anti- progestin RU486 significantly elevated GM-CSF output in cells from ovariectomized mice given estrogen and progesterone in combination and antagonized the inhibition of GM-CSF release seen in cells harvested from mice treated with hCG. These studies demonstrate that GM-CSF synthesis and/or release by uterine epithelial cells is stimulated by estrogen, with progesterone having a moderate inhibitory effect. Analysis of GM-CSF mRNA expression in uterine epithelial cell cultures and in intact uteri from steroid hormone-treated ovariectomized mice by quantitative reverse transcription-polymerase chain reaction indicated that the effects of estrogen and progesterone on GM-CSF release are mediated at least in part at the transcriptional level. These findings implicate GM-CSF as a local mediator of steroid-driven remodeling events in the cycling and preimplantation endometrium, possibly acting through the recruitment and behavioral regulation of granulocytes and macrophages.
This article has been cited by other articles:
|
|
 |
|
  A. Tonello and G. Poli Tubal ectopic pregnancy: macrophages under the microscope Hum. Reprod., October 1, 2007; 22(10): 2577 - 2584. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Robertson Seminal fluid signaling in the female reproductive tract: Lessons from rodents and pigs J Anim Sci, March 1, 2007; 85(13_suppl): E36 - E44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A.-H. Zarnani, S.-M. Moazzeni, F. Shokri, M. Salehnia, and M. Jeddi-Tehrani Kinetics of murine decidual dendritic cells Reproduction, January 1, 2007; 133(1): 275 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Sekiguchi, J. Kwon, E. Yoshida, H. Hamasaki, S. Ichinose, M. Hideshima, M. Kuraoka, A. Takahashi, Y. Ishii, S. Kyuwa, et al. Localization of Ubiquitin C-Terminal Hydrolase L1 in Mouse Ova and Its Function in the Plasma Membrane to Block Polyspermy Am. J. Pathol., November 1, 2006; 169(5): 1722 - 1729. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gopichandran, U. V Ekbote, J. J Walker, D. Brooke, and N. M Orsi Multiplex determination of murine seminal fluid cytokine profiles. Reproduction, March 1, 2006; 131(3): 613 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Crane-Godreau and C. R. Wira Effects of Estradiol on Lipopolysaccharide and Pam3Cys Stimulation of CCL20/Macrophage Inflammatory Protein 3 Alpha and Tumor Necrosis Factor Alpha Production by Uterine Epithelial Cells in Culture Infect. Immun., July 1, 2005; 73(7): 4231 - 4237. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sjoblom, C. T. Roberts, M. Wikland, and S. A. Robertson Granulocyte-Macrophage Colony-Stimulating Factor Alleviates Adverse Consequences of Embryo Culture on Fetal Growth Trajectory and Placental Morphogenesis Endocrinology, May 1, 2005; 146(5): 2142 - 2153. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Bonello, M. J. Jasper, and R. J. Norman Periovulatory Expression of Intercellular Adhesion Molecule-1 in the Rat Ovary Biol Reprod, October 1, 2004; 71(4): 1384 - 1390. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S O'Leary, M J Jasper, G M Warnes, D T Armstrong, and S A Robertson Seminal plasma regulates endometrial cytokine expression, leukocyte recruitment and embryo development in the pig Reproduction, August 1, 2004; 128(2): 237 - 247. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Patrone, T. N. Cassel, K. Pettersson, Y.-S. Piao, G. Cheng, P. Ciana, A. Maggi, M. Warner, J.-A. Gustafsson, and M. Nord Regulation of Postnatal Lung Development and Homeostasis by Estrogen Receptor {beta} Mol. Cell. Biol., December 1, 2003; 23(23): 8542 - 8552. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sjoblom, M. Wikland, and S. A. Robertson Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) Acts Independently of the Beta Common Subunit of the GM-CSF Receptor to Prevent Inner Cell Mass Apoptosis in Human Embryos Biol Reprod, December 1, 2002; 67(6): 1817 - 1823. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Caballero-Campo, F. Dominguez, J. Coloma, M. Meseguer, J. Remohi, A. Pellicer, and C. Simon Hormonal and embryonic regulation of chemokines IL-8, MCP-1 and RANTES in the human endometrium during the window of implantation Mol. Hum. Reprod., April 1, 2002; 8(4): 375 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Propst, B. J. Quade, R. A. Nowak, and E. A. Stewart Granulocyte Macrophage Colony-Stimulating Factor in Adenomyosis and Autologous Endometrium Reproductive Sciences, March 1, 2002; 9(2): 93 - 97. [Abstract] [PDF]
|
|
|
|
|
|
D. Stygar, H. Wang, Y. S. Vladic, G. Ekman, H. Eriksson, and L. Sahlin Co-localization of oestrogen receptor {beta} and leukocyte markers in the human cervix Mol. Hum. Reprod., September 1, 2001; 7(9): 881 - 886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Auersperg, A. S. T. Wong, K.-C. Choi, S. K. Kang, and P. C. K. Leung Ovarian Surface Epithelium: Biology, Endocrinology, and Pathology Endocr. Rev., April 1, 2001; 22(2): 255 - 288. [Abstract] [Full Text]
|
|
|
|
|
|
S. A. Robertson, C. Sjöblom, M. J. Jasper, R. J. Norman, and R. F. Seamark Granulocyte-Macrophage Colony-Stimulating Factor Promotes Glucose Transport and Blastomere Viability in Murine Preimplantation Embryos Biol Reprod, April 1, 2001; 64(4): 1206 - 1215. [Abstract] [Full Text]
|
|
|
|
|
|
H. Wang, Y. Stjernholm, G. Ekman, H. Eriksson, and L. Sahlin Different regulation of oestrogen receptors {{alpha}} and {beta} in the human cervix at term pregnancy Mol. Hum. Reprod., March 1, 2001; 7(3): 293 - 300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Sjoblom, M. Wikland, and S.A. Robertson Granulocyte-macrophage colony-stimulating factor promotes human blastocyst development in vitro Hum. Reprod., December 1, 1999; 14(12): 3069 - 3076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M.A. Habiba, S.C. Bell, and F. Al-Azzawi The effect of hormone replacement therapy on the number and the proliferation index of endometrial leukocytes Hum. Reprod., December 1, 1999; 14(12): 3088 - 3094. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Chegini, X.-M. Tang, and C. Ma Regulation of Transforming Growth Factor-{beta}1 Expression by Granulocyte Macrophage-Colony-Stimulating Factor in Leiomyoma and Myometrial Smooth Muscle Cells J. Clin. Endocrinol. Metab., November 1, 1999; 84(11): 4138 - 4143. [Abstract] [Full Text]
|
|
|
|
|
|
G. Dirami, N. Ravindranath, V. Pursel, and M. Dym Effects of Stem Cell Factor and Granulocyte Macrophage-Colony Stimulating Factor on Survival of Porcine Type A Spermatogonia Cultured in KSOM Biol Reprod, July 1, 1999; 61(1): 225 - 230. [Abstract] [Full Text]
|
|
|
|
|
|
N. Chegini, X.-M. Tang, and Q. Dou The expression, activity and regulation of granulocyte macrophage-colony stimulating factor in human endometrial epithelial and stromal cells Mol. Hum. Reprod., May 1, 1999; 5(5): 459 - 466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.A. Robertson, C.T. Roberts, K.L. Farr, A.R. Dunn, and R.F. Seamark Fertility Impairment in Granulocyte-Macrophage Colony-Stimulating Factor-Deficient Mice Biol Reprod, February 1, 1999; 60(2): 251 - 261. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. G. Richards, M. P. Walker, J. Sebastian, and R. P. DiAugustine Insulin-like Growth Factor-1 (IGF-1) Receptor-Insulin Receptor Substrate Complexes in the Uterus. ALTERED SIGNALING RESPONSE TO ESTRADIOL IN THE IGF-1m/m MOUSE J. Biol. Chem., May 8, 1998; 273(19): 11962 - 11969. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
