Biology of Reproduction, Vol 55, 1179-1184, Copyright © 1996 by Society for the Study of Reproduction

ARTICLES

beta-mercaptoethanol enhances blastocyst formation rate of bovine in vitro-matured/in vitro-fertilized embryos

JN Caamano, ZY Ryoo, JA Thomas and CR Youngs
Department of Animal Science, Iowa State University, Ames 50011-3150, USA.

The objectives of this research were to determine the effects of beta- mercaptoethanol (beta-ME) and fetal bovine serum (FBS) on in vitro development of bovine embryos derived from oocytes matured and fertilized in vitro and to examine the mechanism through which beta-ME may influence embryo development. A 2 x 2 factorial treatment arrangement was used to evaluate the effect of 0 or 100 microM beta-ME and 0% or 10% FBS on embryos cultured in Medium 199 (M199) in the absence of somatic cells. Embryos were randomly allocated within stage of development (< 8 cells or 8-16 cells) to one of four treatment combinations and were cultured for 6 days. Both beta-ME and FBS promoted increased (p < 0.01) development of embryos to the blastocyst stage, and their effects were greater (p < 0.01) in 8- to 16-cell embryos than in embryos having fewer than 8 cells at the initiation of treatment. The cysteine and cystine content of M199, with and without beta-ME, were determined by HPLC. Medium supplemented with beta-ME contained neither cysteine nor cystine, and it is suggested that these compounds were converted into a mixed disulfide between cysteine and beta-ME. These results indicate that beta-ME is capable of enhancing bovine embryo development in a cell-free, serum-free culture system.

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