| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Biology of Reproduction, Vol 57, 693-699, Copyright © 1997 by Society for the Study of Reproduction
ARTICLES |
DM Duffy, TR Wells, GJ Haluska and RL Stouffer
Division of Reproductive Sciences, Oregon Regional Primate Research Center, Beaverton 97006, USA.
Recent evidence suggests that progesterone is required for ovulation, luteinization, and the maintenance of luteal structure and function in primates. Progesterone action is mediated by intracellular progesterone receptors (PRs), and PRs are detectable by immunocytochemistry in the monkey corpus luteum. However, changes in total luteal PR and PR isoform expression have not been quantitated in the corpus luteum during its life span in the menstrual cycle. This study was initiated to identify and quantify PR isoforms in the macaque corpus luteum throughout the luteal phase of the natural cycle by means of Western blotting. Several antibodies generated against the human PR recognized two bands of consistent molecular weights in monkey tissues, and these bands comigrated with PR-A and PR-B from human T47D cells. Taken together, these data suggest that the two proteins identified were macaque PR-A and PR-B. The estimated molecular weights of monkey PR-A and PR-B were approximately 90,000 and 120,000, respectively. PRs were detected in a variety of macaque tissues, including the endometrium, whole ovary, and decidua, but not in spleen, which is PR-negative by other techniques. Whereas PR-A was the predominant isoform observed in endometrium and decidua, PR-B predominated in the ovary without a dominant follicle or corpus luteum as well as in the corpus luteum. In luteal tissue, PR-A levels decreased (p < 0.05) over the course of the luteal phase, while PR-B levels were unchanged. Hence the ratio of PR-B to PR-A (PR-B:PR-A) increased (p < 0.05) from early to very late luteal phase. Since PR-B:PR-A can alter gene expression in response to progestins and antiprogestins in vitro, the temporal changes in PR-B:PR- A in the monkey corpus luteum may contribute to functional differences in luteal responses to progesterone and other steroids in vivo.
This article has been cited by other articles:
|
|
 |
|
  R. L. Bogan, M. J. Murphy, R. L. Stouffer, and J. D. Hennebold Prostaglandin Synthesis, Metabolism, and Signaling Potential in the Rhesus Macaque Corpus Luteum throughout the Luteal Phase of the Menstrual Cycle Endocrinology, November 1, 2008; 149(11): 5861 - 5871. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Mani Progestin Receptor Subtypes in the Brain: The Known and the Unknown Endocrinology, June 1, 2008; 149(6): 2750 - 2756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Bogan, M. J. Murphy, R. L. Stouffer, and J. D. Hennebold Systematic Determination of Differential Gene Expression in the Primate Corpus Luteum during the Luteal Phase of the Menstrual Cycle Mol. Endocrinol., May 1, 2008; 22(5): 1260 - 1273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu, F. Xu, J. D. Hennebold, T. A. Molskness, and R. L. Stouffer Expression and Role of the Corticotropin-Releasing Hormone/Urocortin-Receptor-Binding Protein System in the Primate Corpus Luteum during the Menstrual Cycle Endocrinology, November 1, 2007; 148(11): 5385 - 5395. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Engmann, R. Losel, M. Wehling, and J. J. Peluso Progesterone Regulation of Human Granulosa/Luteal Cell Viability by an RU486-Independent Mechanism J. Clin. Endocrinol. Metab., December 1, 2006; 91(12): 4962 - 4968. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Tesone, R. L. Stouffer, S. M. Borman, J. D. Hennebold, and T. A. Molskness Vascular Endothelial Growth Factor (VEGF) Production by the Monkey Corpus Luteum During the Menstrual Cycle: Isoform-Selective Messenger RNA Expression In Vivo and Hypoxia-Regulated Protein Secretion In Vitro Biol Reprod, November 1, 2005; 73(5): 927 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Peluso, A. Pappalardo, R. Losel, and M. Wehling Expression and Function of PAIRBP1 Within Gonadotropin-Primed Immature Rat Ovaries: PAIRBP1 Regulation of Granulosa and Luteal Cell Viability Biol Reprod, August 1, 2005; 73(2): 261 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W C. Duncan, E. Gay, and J. A Maybin The effect of human chorionic gonadotrophin on the expression of progesterone receptors in human luteal cells in vivo and in vitro Reproduction, July 1, 2005; 130(1): 83 - 93. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Duffy, B. L. Dozier, and C. L. Seachord Prostaglandin Dehydrogenase and Prostaglandin Levels in Periovulatory Follicles: Implications for Control of Primate Ovulation by Prostaglandin E2 J. Clin. Endocrinol. Metab., February 1, 2005; 90(2): 1021 - 1027. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A Maybin and W C. Duncan The human corpus luteum: which cells have progesterone receptors? Reproduction, October 1, 2004; 128(4): 423 - 431. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mulac-Jericevic and O. M Conneely Reproductive tissue selective actions of progesterone receptors Reproduction, August 1, 2004; 128(2): 139 - 146. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Gava, C. L. Clarke, K. Byth, R. L. Arnett-Mansfield, and A. deFazio Expression of Progesterone Receptors A and B in the Mouse Ovary during the Estrous Cycle Endocrinology, July 1, 2004; 145(7): 3487 - 3494. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Borman, C. L. Chaffin, K. M. Schwinof, R. L. Stouffer, and M. B. Zelinski-Wooten Progesterone Promotes Oocyte Maturation, but Not Ovulation, in Nonhuman Primate Follicles Without a Gonadotropin Surge Biol Reprod, July 1, 2004; 71(1): 366 - 373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. M. Duffy Growth Differentiation Factor-9 Is Expressed by the Primate Follicle Throughout the Periovulatory Interval Biol Reprod, August 1, 2003; 69(2): 725 - 732. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Goyeneche, R. P. Deis, G. Gibori, and C. M. Telleria Progesterone Promotes Survival of the Rat Corpus Luteum in the Absence of Cognate Receptors Biol Reprod, January 1, 2003; 68(1): 151 - 158. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Jo, C.M. Komar, and J.E. Fortune Gonadotropin Surge Induces Two Separate Increases in Messenger RNA for Progesterone Receptor in Bovine Preovulatory Follicles Biol Reprod, December 1, 2002; 67(6): 1981 - 1988. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Haluska, T. R. Wells, J. J. Hirst, R. M. Brenner, D. W. Sadowsky, and M. J. Novy Progesterone receptor Localization and Isoforms in Myometrium, Decidua, and Fetal Membranes From Rhesus Macaques: Evidence for Function Progresterone Withdrawal at Parturtion Reproductive Sciences, May 1, 2002; 9(3): 125 - 136. [Abstract] [PDF]
|
|
|
|
 |
|
 O. M. Conneely, B. Mulac-Jericevic, F. DeMayo, J. P. Lydon, and B. W. O'Malley Reproductive Functions of Progesterone Receptors Recent Prog. Horm. Res., January 1, 2002; 57(1): 339 - 355. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. M. Conneely Perspective: Female Steroid Hormone Action Endocrinology, June 1, 2001; 142(6): 2194 - 2199. [Full Text] [PDF]
|
|
|
|
|
|
A. Mayerhofer, S. Fritz, R. Grünert, S. L. Sanders, D. M. Duffy, S. R. Ojeda, and R. L. Stouffer D1-Receptor, DARPP-32, and PP-1 in the Primate Corpus Luteum and Luteinized Granulosa Cells: Evidence for Phosphorylation of DARPP-32 by Dopamine and Human Chorionic Gonadotropin J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4750 - 4757. [Abstract] [Full Text]
|
|
|
|
 |
|
 B. Mulac-Jericevic, R. A. Mullinax, F. J. DeMayo, J. P. Lydon, and O. M. Conneely Subgroup of Reproductive Functions of Progesterone Mediated by Progesterone Receptor-B Isoform Science, September 8, 2000; 289(5485): 1751 - 1754. [Abstract] [Full Text]
|
|
|
|
|
|
B. D. Murphy Models of Luteinization Biol Reprod, July 1, 2000; 63(1): 2 - 11. [Abstract] [Full Text]
|
|
|
|
|
|
D. M. Duffy, C. L. Chaffin, and R. L. Stouffer Expression of Estrogen Receptor {alpha} and {beta} in the Rhesus Monkey Corpus Luteum during the Menstrual Cycle: Regulation by Luteinizing Hormone and Progesterone Endocrinology, May 1, 2000; 141(5): 1711 - 1717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Ottander, K. Hosokawa, K. Liu, A. Bergh, T. Ny, and J. I. Olofsson A Putative Stimulatory Role of Progesterone Acting via Progesterone Receptors in the Steroidogenic Cells of the Human Corpus Luteum Biol Reprod, March 1, 2000; 62(3): 655 - 663. [Abstract] [Full Text]
|
|
|
|
|
|
B. R. Rueda, I. R. Hendry, W. J. Hendry III, F. Stormshak, O.D. Slayden, and J. S. Davis Decreased Progesterone Levels and Progesterone Receptor Antagonists Promote Apoptotic Cell Death in Bovine Luteal Cells Biol Reprod, February 1, 2000; 62(2): 269 - 276. [Abstract] [Full Text]
|
|
|
|
|
|
M. Szabo, S. M. Kilen, S. J. Nho, and N. B. Schwartz Progesterone Receptor A and B Messenger Ribonucleic Acid Levels in the Anterior Pituitary of Rats Are Regulated by Estrogen Biol Reprod, January 1, 2000; 62(1): 95 - 102. [Abstract] [Full Text]
|
|
|
|
|
|
D. M. Duffy, D. R. Stewart, and R. L. Stouffer Titrating Luteinizing Hormone Replacement to Sustain the Structure and Function of the Corpus Luteum after Gonadotropin-Releasing Hormone Antagonist Treatment in Rhesus Monkeys J. Clin. Endocrinol. Metab., January 1, 1999; 84(1): 342 - 349. [Abstract] [Full Text]
|
|
|
|
|
|
C. L. Bethea and A. A. Widmann Differential Expression of Progestin Receptor Isoforms in the Hypothalamus, Pituitary, and Endometrium of Rhesus Macaques Endocrinology, February 1, 1998; 139(2): 677 - 687. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
