Biology of Reproduction, Vol 57, 723-728, Copyright © 1997 by Society for the Study of Reproduction

ARTICLES

Up-regulation of oxytocin receptors in porcine endometrium by adenosine 3',5'-monophosphate

Y Uenoyama, S Hattori, M Miyake and K Okuda
Laboratory of Reproductive Endocrinology, Faculty of Agriculture, Okayama University, Japan.

Porcine endometrial cells (a mixture of epithelial, stromal, and glandular cells) were examined for the presence of oxytocin (OT) receptors using a cell culture system and a 125I-labeled OT antagonist. Binding specificity was tested in displacement studies with various related peptides. Scatchard analyses revealed the presence of a binding site with a dissociation constant (Kd) = 0.9 nM and a capacity of 1.9 fmol/10(5) cells. These cells, which were obtained from prepubertal gilts and thus had not been exposed to endogenous ovarian steroids, were used as a model to evaluate the possible action of ovarian steroids and intracellular cAMP on OT receptors. Although ovarian steroids showed no effect on OT receptors, forskolin (an adenylate cyclase activator) and dibutyryl cAMP caused 1.5- to 1.6-fold increases in specific binding of OT without changing the binding affinity. When the endometrial cells were exposed to OT (0.1-1000 nM) in combination with arachidonic acid (10 microM), OT stimulated prostaglandin F2 alpha secretion in a dose-dependent manner. These results indicate the presence of functional OT receptors in prepubertal porcine endometrial cells and suggest that the concentration of OT receptors may be regulated by one or more substances that raise intracellular cAMP levels.

This article has been cited by other articles:

				M. Tanikawa, H.-Y. Lee, K. Watanabe, M. Majewska, D. J Skarzynski, S.-B. Park, D.-S. Lee, C.-K. Park, T. J Acosta, and K. Okuda Regulation of prostaglandin biosynthesis by interleukin-1 in cultured bovine endometrial cells J. Endocrinol., December 1, 2008; 199(3): 425 - 434. [Abstract] [Full Text] [PDF]

				A. Waclawik and A. J Ziecik Differential expression of prostaglandin (PG) synthesis enzymes in conceptus during peri-implantation period and endometrial expression of carbonyl reductase/PG 9-ketoreductase in the pig J. Endocrinol., September 1, 2007; 194(3): 499 - 510. [Abstract] [Full Text] [PDF]

				A. Blitek and A. J Ziecik Effect of LH on prostaglandin F2{alpha} and prostaglandin E2 secretion by cultured porcine endometrial cells Reproduction, July 1, 2005; 130(1): 105 - 112. [Abstract] [Full Text] [PDF]

				K. Okuda, Y. Kasahara, S. Murakami, H. Takahashi, I. Woclawek-Potocka, and D. J. Skarzynski Interferon-{tau} Blocks the Stimulatory Effect of Tumor Necrosis Factor-{alpha} on Prostaglandin F2{alpha} Synthesis by Bovine Endometrial Stromal Cells Biol Reprod, January 1, 2004; 70(1): 191 - 197. [Abstract] [Full Text] [PDF]

				M. Bogacki, W.J. Silvia, R. Rekawiecki, and J. Kotwica Direct Inhibitory Effect of Progesterone on Oxytocin-Induced Secretion of Prostaglandin F2{alpha} from Bovine Endometrial Tissue Biol Reprod, July 1, 2002; 67(1): 184 - 188. [Abstract] [Full Text] [PDF]

				K. Okuda, Y. Uenoyama, B. Berisha, I. G. Lange, H. Taniguchi, S. Kobayashi, S.-i. Kobayashi, A. Miyamoto, and D. Schams Estradiol-17{beta} Is Produced in Bovine Corpus Luteum Biol Reprod, December 1, 2001; 65(6): 1634 - 1639. [Abstract] [Full Text] [PDF]

				D. J. Skarzynski, S. Kobayashi, and K. Okuda Influence of Nitric Oxide and Noradrenaline on Prostaglandin F2{alpha}-Induced Oxytocin Secretion and Intracellular Calcium Mobilization in Cultured Bovine Luteal Cells Biol Reprod, October 1, 2000; 63(4): 1000 - 1005. [Abstract] [Full Text]

				Y. Miyamoto, D. J. Skarzynski, and K. Okuda Is Tumor Necrosis Factor {alpha} a Trigger for the Initiation of Endometrial Prostaglandin F2{alpha} Release at Luteolysis in Cattle? Biol Reprod, May 1, 2000; 62(5): 1109 - 1115. [Abstract] [Full Text]

				D. J. Skarzynski, Y. Miyamoto, and K. Okuda Production of Prostaglandin F2{alpha} by Cultured Bovine Endometrial Cells in Response to Tumor Necrosis Factor {alpha}: Cell Type Specificity and Intracellular Mechanisms Biol Reprod, May 1, 2000; 62(5): 1116 - 1120. [Abstract] [Full Text]

				R. Sakumoto, B. Berisha, N. Kawate, D. Schams, and K. Okuda Tumor Necrosis Factor-{alpha} and Its Receptor in Bovine Corpus Luteum Throughout the Estrous Cycle Biol Reprod, January 1, 2000; 62(1): 192 - 199. [Abstract] [Full Text]

				D. J. Skarzynski and K. Okuda Sensitivity of Bovine Corpora Lutea to Prostaglandin F2{alpha} Is Dependent on Progesterone, Oxytocin, and Prostaglandins Biol Reprod, June 1, 1999; 60(6): 1292 - 1298. [Abstract] [Full Text]

				D. J Skarzynski, Y. Uenoyama, J. Kotwica, and K. Okuda Noradrenaline Stimulates the Production of Prostaglandin F2{alpha} in Cultured Bovine Endometrial Cells Biol Reprod, February 1, 1999; 60(2): 277 - 282. [Abstract] [Full Text] [PDF]