Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Amleh, A.
Right arrow Articles by Taketo, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Amleh, A.
Right arrow Articles by Taketo, T.
Agricola
Right arrow Articles by Amleh, A.
Right arrow Articles by Taketo, T.

Biology of Reproduction, Vol 58, 574-582, Copyright © 1998 by Society for the Study of Reproduction

ARTICLES

Live-borns from XX but not XY oocytes in the chimeric mouse ovary composed of B6.Y(TIR) and XX cells

A Amleh and T Taketo
Department of Biology, McGill University, Royal Victoria Hospital, Montreal, Quebec, Canada.

When the Y chromosome of some Mus musculus domesticus subspecies is placed onto a C57BL/6J mouse background, the XY (B6.Y(TIR)) progeny develop only ovaries or ovotestes during fetal life. The XY sex- reversed female is infertile mainly because of death of embryos during preimplantation development. In the present study, we constructed female mouse chimera composed of B6.Y(TIR) and XX BALB/c cells to determine whether developmental incompetence of XY oocytes can be attributed to defects in the oocytes themselves or in the surrounding XY somatic cells. Distribution of XY cells in chimeric ovaries was examined by in situ hybridization. Of nine XX <--> XY chimeric females born, eight were composed of B6.Y(TIR) and XX BALB/c cells with a wide range of XY contribution (16-95%), whereas one had 12% XY components of the BALB/c strain. All these females produced progeny exclusively derived from XX oocytes. By comparison, most XX <--> XX chimeric females produced progeny derived from oocytes of either strain. Two XY <--> XY males also produced progeny of both strains. In conclusion, the XY chromosomal composition in the oocyte appears to be responsible for programming its incompetence for postfertilization development. On the other hand, the presence of XY somatic cells in the chimeric ovary allows development of fertile XX oocytes.


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
Y. Obata, M. Villemure, T. Kono, and T. Taketo
Transmission of Y chromosomes from XY female mice was made possible by the replacement of cytoplasm during oocyte maturation
PNAS, September 16, 2008; 105(37): 13918 - 13923.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
M. Alton, M. P. Lau, M. Villemure, and T. Taketo
The behavior of the X- and Y-chromosomes in the oocyte during meiotic prophase in the B6.YTIR sex-reversed mouse ovary
Reproduction, February 1, 2008; 135(2): 241 - 252.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
E.-H. Park and T. Taketo
Onset and Progress of Meiotic Prophase in the Oocytes in the B6.YTIR Sex-Reversed Mouse Ovary
Biol Reprod, December 1, 2003; 69(6): 1879 - 1889.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
C.A. Hodges, A. Ilagan, D. Jennings, R. Keri, J. Nilson, and P.A. Hunt
Experimental evidence that changes in oocyte growth influence meiotic chromosome segregation
Hum. Reprod., May 1, 2002; 17(5): 1171 - 1180.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Society for the Study of Reproduction.