Biology of Reproduction, Vol 58, 591-599, Copyright © 1998 by Society for the Study of Reproduction

ARTICLES

Characterization of a clonal Sertoli cell line using adult PyLT transgenic mice

V Bourdon, A Lablack, P Abbe, D Segretain and G Pointis
Groupe de Recherche sur l'Interaction Gametique, CJF INSERM 95/04, Faculte de Medecine, Nice, France.

In the present study we report the isolation and characterization of a clonal Sertoli cell line (42GPA9) from sexually mature polyoma virus large T (PyLT) transgenic mice. The cells multiplied indefinitely and expressed large T antigen. The 42GPA9 cell line expressed biochemical features associated with normal Sertoli cells. Transferrin, sulfated glycoprotein-2, and the ligand of c-kit were detected by reverse transcription-polymerase chain reactions and Western blot analyses. Zymographic analysis indicated that the 42GPA9 cell line secreted tissue-type plasminogen activator. These cells also retained FSH receptors as suggested by their specific responsiveness to the gonadotropin (morphological and phagocytic changes, stimulation of cAMP production) and the detection of FSH receptor mRNAs. Another original aspect of the 42GPA9 cell line is its ability to form tight junctions at confluency as demonstrated by electron microscopic study and immunolocalization of the tight junction-associated protein zonula occludens 1. The 42GPA9 cell line, which has retained several important hallmarks of normal Sertoli cells, may prove useful for further studies on Sertoli cell behavior and on Sertoli-germ cell interactions in the mature testis.

This article has been cited by other articles:

				E. Corcelle, M. Nebout, S. Bekri, N. Gauthier, P. Hofman, P. Poujeol, P. Fenichel, and B. Mograbi Disruption of Autophagy at the Maturation Step by the Carcinogen Lindane Is Associated with the Sustained Mitogen-Activated Protein Kinase/Extracellular Signal-Regulated Kinase Activity. Cancer Res., July 1, 2006; 66(13): 6861 - 6870. [Abstract] [Full Text] [PDF]

				C. Fiorini, X. Decrouy, N. Defamie, D. Segretain, and G. Pointis Opposite regulation of connexin33 and connexin43 by LPS and IL-1{alpha} in spermatogenesis Am J Physiol Cell Physiol, March 1, 2006; 290(3): C733 - C740. [Abstract] [Full Text] [PDF]

				J. Chaudhary, I. Sadler-Riggleman, J. M. Ague, and M. K. Skinner The Helix-Loop-Helix Inhibitor of Differentiation (ID) Proteins Induce Post-Mitotic Terminally Differentiated Sertoli Cells to Re-Enter the Cell Cycle and Proliferate Biol Reprod, May 1, 2005; 72(5): 1205 - 1217. [Abstract] [Full Text] [PDF]

				C. Fiorini, B. Mograbi, L. Cronier, I. Bourget, X. Decrouy, M. Nebout, B. Ferrua, A. Malassine, M. Samson, P. Fenichel, et al. Dominant negative effect of connexin33 on gap junctional communication is mediated by connexin43 sequestration J. Cell Sci., September 15, 2004; 117(20): 4665 - 4672. [Abstract] [Full Text] [PDF]

				B. Mograbi, E. Corcelle, N. Defamie, M. Samson, M. Nebout, D. Segretain, P. Fenichel, and G. Pointis Aberrant Connexin 43 endocytosis by the carcinogen lindane involves activation of the ERK/mitogen-activated protein kinase pathway Carcinogenesis, August 1, 2003; 24(8): 1415 - 1423. [Abstract] [Full Text] [PDF]

				N. Defamie, B. Mograbi, C. Roger, L. Cronier, A. Malassine, F. Brucker-Davis, P. Fenichel, D. Segretain, and G. Pointis Disruption of gap junctional intercellular communication by lindane is associated with aberrant localization of connexin43 and zonula occludens-1 in 42GPA9 Sertoli cells Carcinogenesis, September 1, 2001; 22(9): 1537 - 1542. [Abstract] [Full Text] [PDF]

				W. Xing and M. Ram Sairam Characterization of Regulatory Elements of Ovine Follicle-Stimulating Hormone (FSH) Receptor Gene: The Role of E-Box in the Regulation of Ovine FSH Receptor Expression Biol Reprod, February 1, 2001; 64(2): 579 - 589. [Abstract] [Full Text]

				C. Batias, J.-P. Siffroi, P. Fénichel, G. Pointis, and D. Segretain Connexin43 Gene Expression and Regulation in the Rodent Seminiferous Epithelium J. Histochem. Cytochem., June 1, 2000; 48(6): 793 - 806. [Abstract] [Full Text]