Biology of Reproduction, Vol 58, 834-841, Copyright © 1998 by Society for the Study of Reproduction

ARTICLES

Glyceraldehyde 3-phosphate dehydrogenase-S protein distribution during mouse spermatogenesis

DO Bunch, JE Welch, PL Magyar, EM Eddy and DA O'Brien
Laboratory of Reproductive and Developmental Toxicology, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina 27709, USA. bunch@niehs.nih.gov

The spermatogenic cell-specific isoform of glyceraldehyde 3-phosphate dehydrogenase (GAPD-S) may regulate glycolysis and energy production required for sperm motility. Although the steady-state level of Gapd-s mRNA is maximal at step 9 of mouse spermatogenesis, GAPD-S protein was not detected by immunohistochemistry until steps 12-13. This result suggests that Gapd-s is translationally regulated. Western blot analysis of isolated germ cells confirmed that GAPD-S is not detected in pachytene spermatocytes or round spermatids. A major immunoreactive protein migrating with a molecular weight (M(r)) of 69,200 was observed in condensing spermatids and cauda sperm. Additional minor proteins that migrated at M(r) 55,200, 32,500, and 27,500 were detected in sperm. The molecular weight of GAPD-S is higher than the predicted molecular weight of 47,445, apparently due to a proline-rich 105-amino acid domain at the N-terminus. Recombinant GAPD-S protein lacking the proline-rich region migrated at M(r) 38,250, comparably to somatic GAPD, which also lacks the proline-rich domain. Indirect immunofluorescence demonstrated that GAPD-S is restricted to the principal piece in the sperm flagellum. Western blot analysis indicated that GAPD-S is tightly associated with the fibrous sheath of the flagellum, consistent with a potential role in regulating sperm motility.

This article has been cited by other articles:

				B. Muciaccia, S. Corallini, E. Vicini, F. Padula, L. Gandini, G. Liuzzi, A. Lenzi, and M. Stefanini HIV-1 viral DNA is present in ejaculated abnormal spermatozoa of seropositive subjects Hum. Reprod., November 1, 2007; 22(11): 2868 - 2878. [Abstract] [Full Text] [PDF]

				S. Feiden, H. Stypa, U. Wolfrum, G. Wegener, and G. Kamp A novel pyruvate kinase (PK-S) from boar spermatozoa is localized at the fibrous sheath and the acrosome Reproduction, July 1, 2007; 134(1): 81 - 95. [Abstract] [Full Text] [PDF]

				G Kamp, H Schmidt, H Stypa, S Feiden, C Mahling, and G Wegener Regulatory properties of 6-phosphofructokinase and control of glycolysis in boar spermatozoa Reproduction, January 1, 2007; 133(1): 29 - 40. [Abstract] [Full Text] [PDF]

				W. Cao, L. Haig-Ladewig, G. L. Gerton, and S. B. Moss Adenylate Kinases 1 and 2 Are Part of the Accessory Structures in the Mouse Sperm Flagellum Biol Reprod, October 1, 2006; 75(4): 492 - 500. [Abstract] [Full Text] [PDF]

				M. Krisfalusi, K. Miki, P. L. Magyar, and D. A. O'Brien Multiple Glycolytic Enzymes Are Tightly Bound to the Fibrous Sheath of Mouse Spermatozoa Biol Reprod, August 1, 2006; 75(2): 270 - 278. [Abstract] [Full Text] [PDF]

				W. Cao, G. L. Gerton, and S. B. Moss Proteomic Profiling of Accessory Structures from the Mouse Sperm Flagellum Mol. Cell. Proteomics, May 1, 2006; 5(5): 801 - 810. [Abstract] [Full Text] [PDF]

				K. Miki, W. Qu, E. H. Goulding, W. D. Willis, D. O. Bunch, L. F. Strader, S. D. Perreault, E. M. Eddy, and D. A. O'Brien Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility PNAS, November 23, 2004; 101(47): 16501 - 16506. [Abstract] [Full Text] [PDF]

				A. Miranda-Vizuete, K. Tsang, Y. Yu, A. Jimenez, M. Pelto-Huikko, C. J. Flickinger, P. Sutovsky, and R. Oko Cloning and Developmental Analysis of Murid Spermatid-specific Thioredoxin-2 (SPTRX-2), a Novel Sperm Fibrous Sheath Protein and Autoantigen J. Biol. Chem., November 7, 2003; 278(45): 44874 - 44885. [Abstract] [Full Text] [PDF]

				R. M. Turner Tales From the Tail: What Do We Really Know About Sperm Motility? J Androl, November 1, 2003; 24(6): 790 - 803. [Full Text] [PDF]

				S. Hayashi, J. Yang, L. Christenson, R. Yanagimachi, and N. B. Hecht Mouse Preimplantation Embryos Developed from Oocytes Injected with Round Spermatids or Spermatozoa Have Similar but Distinct Patterns of Early Messenger RNA Expression Biol Reprod, October 1, 2003; 69(4): 1170 - 1176. [Abstract] [Full Text] [PDF]

				P. R. Brown, K. Miki, D. B. Harper, and E. M. Eddy A-Kinase Anchoring Protein 4 Binding Proteins in the Fibrous Sheath of the Sperm Flagellum Biol Reprod, June 1, 2003; 68(6): 2241 - 2248. [Abstract] [Full Text] [PDF]

				G. Yang, Y.-L. Zhang, G. M. Buchold, A. M. Jetten, and D. A. O'Brien Analysis of Germ Cell Nuclear Factor Transcripts and Protein Expression During Spermatogenesis Biol Reprod, May 1, 2003; 68(5): 1620 - 1630. [Abstract] [Full Text] [PDF]

				J. Yang, V. Chennathukuzhi, K. Miki, D. A. O'Brien, and N. B. Hecht Mouse Testis Brain RNA-Binding Protein/Translin Selectively Binds to the Messenger RNA of the Fibrous Sheath Protein Glyceraldehyde 3-Phosphate Dehydrogenase-S and Suppresses Its Translation In Vitro Biol Reprod, March 1, 2003; 68(3): 853 - 859. [Abstract] [Full Text] [PDF]

				Y. Yu, R. Oko, and A. Miranda-Vizuete Developmental Expression of Spermatid-Specific Thioredoxin-1 Protein: Transient Association to the Longitudinal Columns of the Fibrous Sheath During Sperm Tail Formation Biol Reprod, November 1, 2002; 67(5): 1546 - 1554. [Abstract] [Full Text] [PDF]

				F. Giorgini, H. G. Davies, and R. E. Braun Translational repression by MSY4 inhibits spermatid differentiation in mice Development, August 1, 2002; 129(15): 3669 - 3679. [Abstract] [Full Text] [PDF]

				E. M. Eddy Male Germ Cell Gene Expression Recent Prog. Horm. Res., January 1, 2002; 57(1): 103 - 128. [Abstract] [Full Text] [PDF]

				R. D. Catalano, E. W. Hillhouse, and M. Vlad Developmental Expression and Characterization of FS39, a Testis Complementary DNA Encoding an Intermediate Filament-Related Protein of the Sperm Fibrous Sheath Biol Reprod, July 1, 2001; 65(1): 277 - 287. [Abstract] [Full Text] [PDF]

				F. Urner, G. Leppens-Luisier, and D. Sakkas Protein Tyrosine Phosphorylation in Sperm During Gamete Interaction in the Mouse: The Influence of Glucose Biol Reprod, May 1, 2001; 64(5): 1350 - 1357. [Abstract] [Full Text]

				Y. Si and M. Okuno Role of Tyrosine Phosphorylation of Flagellar Proteins in Hamster Sperm Hyperactivation Biol Reprod, July 1, 1999; 61(1): 240 - 246. [Abstract] [Full Text]

				K. Miki and E. M. Eddy Identification of Tethering Domains for Protein Kinase A Type Ialpha Regulatory Subunits on Sperm Fibrous Sheath Protein FSC1 J. Biol. Chem., December 18, 1998; 273(51): 34384 - 34390. [Abstract] [Full Text] [PDF]

				A. J. Travis, C. J. Jorgez, T. Merdiushev, B. H. Jones, D. M. Dess, L. Diaz-Cueto, B. T. Storey, G. S. Kopf, and S. B. Moss Functional Relationships between Capacitation-dependent Cell Signaling and Compartmentalized Metabolic Pathways in Murine Spermatozoa J. Biol. Chem., March 2, 2001; 276(10): 7630 - 7636. [Abstract] [Full Text] [PDF]