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Biology of Reproduction, Vol 58, 1071-1074, Copyright © 1998 by Society for the Study of Reproduction


ARTICLES

Cryopreservation and orthotopic transplantation of mouse ovaries: new approach in gamete banking

J Sztein, H Sweet, J Farley and L Mobraaten
The Jackson Laboratory, Bar Harbor, Maine 04609, USA. jms@jax.org

Mouse half ovaries were cryopreserved and orthotopically transplanted into ovariectomized recipients genetically identical to ovary donors except for the coat color gene. Fertility was reestablished in 57% of the female recipients, which became pregnant in an average of 40 days after transplantation of frozen-thawed half ovaries. These experiments demonstrate that ovary cryopreservation can be a very useful option for banking mouse germplasm, or managing subfertile animal colonies, when embryo or sperm freezing cannot be used or is not cost effective.


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Copyright © 1998 by the Society for the Study of Reproduction.