Biol Reprod Keystone Symposia Conference on Frontiers in Reproductive Biology & Regulation of Fertility.
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Biology of Reproduction, Vol 58, 927-934, Copyright © 1998 by Society for the Study of Reproduction

ARTICLES

Immunoregulatory activity in adult rat testicular interstitial fluid: roles of interleukin-1 and transforming growth factor beta

MP Hedger, DJ Nikolic-Paterson, P Hutchinson, RC Atkins and DM de Kretser
Institute of Reproduction and Development, Monash University, Monash Medical Centre, Clayton, Victoria, Australia. mark.hedger@med.monash.edu.au

Studies on the effect of rat testicular interstitial fluid (IF) on T- cell function have reported both stimulatory and inhibitory actions. Specific cytokines produced within the testis, particularly interleukin- 1 (IL-1) and transforming growth factor beta (TGFbeta), may contribute to these apparently conflicting observations. In proliferation assays employing lectin- or antibody-activated thymocytes or mature T cells in vitro, adult rat testicular IF stimulated T-cell activation and/or proliferation at low assay doses and was inhibitory at higher doses. The stimulatory activity was blocked by recombinant IL-1 receptor antagonist. The inhibitory activity was not affected by a polyspecific TGFbeta antiserum. The biological characteristics of the inhibitor were distinct from those of a similar, but considerably less potent, activity in platelet-depleted serum. These data demonstrate that rat testicular IF contains biologically significant concentrations of IL-1 but has a predominantly inhibitory action on T-cell responses. The factor predominantly responsible for this inhibitory activity displays a relatively large apparent molecular weight, is protease sensitive and partially heat labile, but does not appear to be one of the known mammalian TGFbeta isoforms.


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Copyright © 1998 by the Society for the Study of Reproduction.