Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gaemers, I. C.
Right arrow Articles by de Rooij, D. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gaemers, I. C.
Right arrow Articles by de Rooij, D. G.
Agricola
Right arrow Articles by Gaemers, I. C.
Right arrow Articles by de Rooij, D. G.

Biology of Reproduction, Vol 58, 1351-1356, Copyright © 1998 by Society for the Study of Reproduction

ARTICLES

Differential expression pattern of retinoid X receptors in adult murine testicular cells implies varying roles for these receptors in spermatogenesis [In Process Citation]

IC Gaemers, AM van Pelt, PT van der Saag, JW Hoogerbrugge, AP Themmen and DG de Rooij
Department of Cell Biology, Utrecht University, The Netherlands. gaemers@nki.nl

Retinoids have previously been shown to be crucial for normal spermatogenesis. The role of retinoic acid receptors has been studied, but relatively little is known about the function of retinoid X receptors (RXRs). To gain more insight in the function of RXRs during spermatogenesis, the cellular localization of RXRs in the mouse testis was examined using immunohistochemistry and RNase protection assays. In both normal and vitamin A-deficient (VAD) testes, a strong immune response to an RXRalpha antibody occurred in Leydig cells, peritubular myoid cells, and A spermatogonia. Weaker signals were found in spermatocytes and spermatids. In normal testes, an RXRbeta antibody gave a reaction in Leydig cells, and, to a lesser extent, in Sertoli cells, A spermatogonia, pachytene spermatocytes, and spermatids. In Leydig cells, a cytoplasmatic signal was found in addition to the nuclear signal. In the VAD testis, only Leydig cells and A spermatogonia were positive, which indicates that RXRbeta expression may be dependent on the retinoid status. Previous studies have shown RXRgamma mRNA expression in the mouse testis at a low level. Nevertheless, an RXRgamma antibody caused a strong immune response in interstitial cells and in A spermatogonia, and a weak signal in pachytene spermatocytes. These immunohistochemical data were supported by the results of RNase protection assays on mRNA of testicular cell isolations. In conclusion, the different RXRs in the mouse testis have distinct expression patterns, suggesting that they may have different functions.


This article has been cited by other articles:


Home page
 ReproductionHome page
N. Vernet, C. Dennefeld, M. Klopfenstein, A. Ruiz, D. Bok, N. B Ghyselinck, and M. Mark
Retinoid X receptor beta (RXRB) expression in Sertoli cells controls cholesterol homeostasis and spermiation
Reproduction, November 1, 2008; 136(5): 619 - 626.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
P. Rantakari, L. Strauss, R. Kiviranta, H. Lagerbohm, J. Paviala, I. Holopainen, S. Vainio, P. Pakarinen, and M. Poutanen
Placenta Defects and Embryonic Lethality Resulting from Disruption of Mouse Hydroxysteroid (17-{beta}) Dehydrogenase 2 Gene
Mol. Endocrinol., March 1, 2008; 22(3): 665 - 675.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Vernet, C. Dennefeld, C. Rochette-Egly, M. Oulad-Abdelghani, P. Chambon, N. B. Ghyselinck, and M. Mark
Retinoic Acid Metabolism and Signaling Pathways in the Adult and Developing Mouse Testis
Endocrinology, January 1, 2006; 147(1): 96 - 110.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
J.P. Dadoune, A. Pawlak, M.F. Alfonsi, and J.P. Siffroi
Identification of transcripts by macroarrays, RT-PCR and in situ hybridization in human ejaculate spermatozoa
Mol. Hum. Reprod., February 1, 2005; 11(2): 133 - 140.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Gazouli, Z.-X. Yao, N. Boujrad, J. C. Corton, M. Culty, and V. Papadopoulos
Effect of Peroxisome Proliferators on Leydig Cell Peripheral-Type Benzodiazepine Receptor Gene Expression, Hormone-Stimulated Cholesterol Transport, and Steroidogenesis: Role of the Peroxisome Proliferator-Activator Receptor {alpha}
Endocrinology, July 1, 2002; 143(7): 2571 - 2583.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Maiti, M. L. Meistrich, G. Wilson, G. Shetty, M. Marcelli, M. J. McPhaul, P. L. Morris, and M. F. Wilkinson
Irradiation Selectively Inhibits Expression from the Androgen-Dependent Pem Homeobox Gene Promoter in Sertoli Cells
Endocrinology, April 1, 2001; 142(4): 1567 - 1577.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
J. M. Dufour and K. H. Kim
Cellular and Subcellular Localization of Six Retinoid Receptors in Rat Testis During Postnatal Development: Identification of Potential Heterodimeric Receptors
Biol Reprod, November 1, 1999; 61(5): 1300 - 1308.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
Y. Morita and J. L. Tilly
Segregation of Retinoic Acid Effects on Fetal Ovarian Germ Cell Mitosis Versus Apoptosis by Requirement for New Macromolecular Synthesis
Endocrinology, June 1, 1999; 140(6): 2696 - 2703.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Society for the Study of Reproduction.