| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Anatomy, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853
Sperm binding to oviductal epithelium produces a reservoir in vivo that may serve to maintain sperm fertility and provide sperm for fertilization when ovulation occurs. Previously, it was determined that bull sperm binding could be blocked by fucoidan and its component fucose; furthermore, treatment of epithelium with fucosidase prevented binding. The present study was conducted to further characterize binding. Because fucose would probably be present on the epithelium as part of oligosaccharide moieties of glycoproteins and/or glycolipids, competitive binding inhibition activity was tested for fucose in five linkages commonly found in oligosaccharides. Binding inhibition was assayed by determining the concentration of motile, frozen/thawed sperm bound to fresh epithelial explants in the presence of test inhibitors. Initially, 5 monosaccharides were tested at 30 mM (fucose, mannose, sialic acid, glucose, N-acetyl glucosamine, and galactose), and only fucose significantly reduced sperm binding compared to vehicle control (p= 0.03). Of the oligosaccharides tested (lacto-N-fucopentaose I, 3-fucosyllactose, Lewis-X, Lewis-a, and GlcNAcß1-4[Fuc
1-6]GlcNAc-O-Me), only Lewis-a significantly reduced binding, and it did so in a dose-dependent fashion (p = 0.009 at 12.5 mM). Ca2+ dependency of binding was examined. Sperm were incubated with explants in Tyrode's albumin lactate pyruvate (TALP) containing 2 mM CaCl2 or lacking CaCl2 and containing 2 mM EGTA. Sperm-binding density was reduced significantly in EGTA (p < 0.03) but could be restored by readdition of CaCl2. Also, live sperm were labeled with the oligosaccharide ligand Lewis-a conjugated to fluorescein isothiocyanate-tagged polyacrylamide. Sperm exhibited labeling on the head only in the presence of Ca2+. Labeling could be blocked by fucose or Lewis-a-polyacrylamide. It was concluded that bull sperm bind to an oligosaccharide ligand on the oviductal epithelium that resembles Lewis-a and that binding is Ca2+-dependent.
2 Correspondence. FAX: (607) 253-3541;sss7{at}cornell.edu
This article has been cited by other articles:
|
|
 |
|
  G. G Ignotz, M. Y Cho, and S. S Suarez Annexins Are Candidate Oviductal Receptors for Bovine Sperm Surface Proteins and Thus May Serve to Hold Bovine Sperm in the Oviductal Reservoir Biol Reprod, December 1, 2007; 77(6): 906 - 913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Ekhlasi-Hundrieser, K. Gohr, A. Wagner, M. Tsolova, A. Petrunkina, and E. Topfer-Petersen Spermadhesin AQN1 Is a Candidate Receptor Molecule Involved in the Formation of the Oviductal Sperm Reservoir in the Pig Biol Reprod, September 1, 2005; 73(3): 536 - 545. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Sostaric, C. H.A. van de Lest, B. Colenbrander, and B. M. Gadella Dynamics of Carbohydrate Affinities at the Cell Surface of Capacitating Bovine Sperm Cells Biol Reprod, February 1, 2005; 72(2): 346 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Boilard, C. Reyes-Moreno, C. Lachance, L. Massicotte, J.L. Bailey, M.-A. Sirard, and P. Leclerc Localization of the Chaperone Proteins GRP78 and HSP60 on the Luminal Surface of Bovine Oviduct Epithelial Cells and Their Association with Spermatozoa Biol Reprod, December 1, 2004; 71(6): 1879 - 1889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. M. Gwathmey, G. G. Ignotz, and S. S. Suarez PDC-109 (BSP-A1/A2) Promotes Bull Sperm Binding to Oviductal Epithelium In Vitro and May Be Involved in Forming the Oviductal Sperm Reservoir Biol Reprod, September 1, 2003; 69(3): 809 - 815. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Zen, Y. Liu, D. Cairo, and C. A. Parkos CD11b/CD18-Dependent Interactions of Neutrophils with Intestinal Epithelium Are Mediated by Fucosylated Proteoglycans J. Immunol., November 1, 2002; 169(9): 5270 - 5278. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Boilard, J. Bailey, S. Collin, M. Dufour, and M.-A. Sirard Effect of Bovine Oviduct Epithelial Cell Apical Plasma Membranes on Sperm Function Assessed by a Novel Flow Cytometric Approach Biol Reprod, October 1, 2002; 67(4): 1125 - 1132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Talevi and R. Gualtieri Sulfated Glycoconjugates Are Powerful Modulators of Bovine Sperm Adhesion and Release from the Oviductal Epithelium In Vitro Biol Reprod, February 1, 2001; 64(2): 491 - 498. [Abstract] [Full Text]
|
|
|
|
|
|
R. Gualtieri and R. Talevi In Vitro-Cultured Bovine Oviductal Cells Bind Acrosome-Intact Sperm and Retain This Ability upon Sperm Release Biol Reprod, June 1, 2000; 62(6): 1754 - 1762. [Abstract] [Full Text]
|
|
|
|
|
|
I. Revah, B. M. Gadella, F. M. Flesch, B. Colenbrander, and S. S. Suárez Physiological State of Bull Sperm Affects Fucose- and Mannose-Binding Properties Biol Reprod, April 1, 2000; 62(4): 1010 - 1015. [Abstract] [Full Text]
|
|
|
|
|
|
P. A. Orihuela, M. E. Ortiz, and H. B. Croxatto Sperm Migration into and through the Oviduct Following Artificial Insemination at Different Stages of the Estrous Cycle in the Rat Biol Reprod, April 1, 1999; 60(4): 908 - 913. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
