| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
Analysis of the biochemical events and the genes expressed at various postnatal developmental stages in the testis of mammals is of great importance for understanding spermatogenesis in general and meiosis in particular. A prerequisite for such an analysis is the characterization of a detailed developmental schedule of the postnatal testis. In this study we used four-parameter flow cytometry analysis to determine a detailed testicular developmental schedule in rats as compared to mice. A dot plot of forward-scatter/side-scatter of testicular cell suspensions from mature animals revealed 7 distinct subpopulations within the testis. These, when analyzed by fluorescence parameters, were divided into 4 levels of fluorescence: cells containing 4d DNA, 2d DNA, and 2 levels of haploid cells. Observing the acquisition pattern of these subpopulations during postnatal development, we were able to suggest the following developmental schedule for the rat. At postnatal Days 6–7, the testis contains somatic cells and spermatogonia cells only. By Days 13–14, leptotene spermatocytes appear; by Days 17–18, zygotene spermatocytes are present; by Days 19–20 and Days 22–23, early and late pachytene spermatocytes, respectively, are seen. Haploid round spermatids first appear at Days 24–25 and elongating spermatids by Days 30–31; by Day 36, elongated spermatozoa can be found.
2 Correspondence. FAX: 972-3-5351824; don{at}ashur.cc.biu.ac.il
This article has been cited by other articles:
|
|
 |
|
  R. Lagos-Cabre and R. D Moreno Mitotic, but not meiotic, oriented cell divisions in rat spermatogenesis Reproduction, April 1, 2008; 135(4): 471 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Silandre, C. Delalande, P. Durand, and S. Carreau Three promoters PII, PI.f, and PI.tr direct the expression of aromatase (cyp19) gene in male rat germ cells J. Mol. Endocrinol., August 1, 2007; 39(2): 169 - 181. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. K. Hamra, K. M. Chapman, D. Nguyen, and D. L. Garbers Identification of Neuregulin as a Factor Required for Formation of Aligned Spermatogonia J. Biol. Chem., January 5, 2007; 282(1): 721 - 730. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Wang, B. L. Sartini, C. F. Millette, and D. L. Kilpatrick A Developmental Switch in Transcription Factor Isoforms During Spermatogenesis Controlled by Alternative Messenger RNA 3'-End Formation Biol Reprod, September 1, 2006; 75(3): 318 - 323. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Thakur, V. Kumar, I. Ghosh, A. Bharadwaj, and K. Datta Appearance of Hyaluronan Binding Protein 1 Proprotein in Pachytene Spermatocytes and Round Spermatids Correlates With Spermatogenesis J Androl, July 1, 2006; 27(4): 604 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Obermann, I. Raabe, M. Balvers, B. Brunswig, W. Schulze, and C. Kirchhoff Novel testis-expressed profilin IV associated with acrosome biogenesis and spermatid elongation Mol. Hum. Reprod., January 1, 2005; 11(1): 53 - 64. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Khetchoumian, M. Teletin, M. Mark, T. Lerouge, M. Cervino, M. Oulad-Abdelghani, P. Chambon, and R. Losson TIF1{delta}, a Novel HP1-interacting Member of the Transcriptional Intermediary Factor 1 (TIF1) Family Expressed by Elongating Spermatids J. Biol. Chem., November 12, 2004; 279(46): 48329 - 48341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Kwon, Y.-L. Wang, R. Setsuie, S. Sekiguchi, M. Sakurai, Y. Sato, W.-W. Lee, Y. Ishii, S. Kyuwa, M. Noda, et al. Developmental Regulation of Ubiquitin C-Terminal Hydrolase Isozyme Expression During Spermatogenesis in Mice Biol Reprod, August 1, 2004; 71(2): 515 - 521. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. E. Szabo, G. P. Pfeifer, and J. R. Mann Parent-of-Origin-Specific Binding of Nuclear Hormone Receptor Complexes in the H19-Igf2 Imprinting Control Region Mol. Cell. Biol., June 1, 2004; 24(11): 4858 - 4868. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-F. Lee, W. S.B. Yeung, J. F.C. Chow, C. K. Shum, and J. M. Luk Different Testicular Gene Expression Patterns in the First Spermatogenic Cycle of Postnatal and Vitamin A-Deficient Rat Testis Biol Reprod, April 1, 2004; 70(4): 1010 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. H. Modarressi, B. Behnam, M. Cheng, K. E. Taylor, J. Wolfe, and F. A. van der Hoorn Tsga10 Encodes a 65-Kilodalton Protein That Is Processed to the 27-Kilodalton Fibrous Sheath Protein Biol Reprod, March 1, 2004; 70(3): 608 - 615. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Xu, A. R. Beyer, W. H. Walker, and E. A. McGee Developmental and Stage-Specific Expression of Smad2 and Smad3 in Rat Testis J Androl, March 1, 2003; 24(2): 192 - 200. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. B. Guo, L. G. Boros, K. C. Chan, A. P. S. Hikim, A. P. Hudson, R. S. Swerdloff, A. P. Mitchell, and W. A. Salameh Spermatogenetic Expression of RNA-Binding Motif Protein 7, a Protein That Interacts With Splicing Factors J Androl, March 1, 2003; 24(2): 204 - 214. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Stelzer and J. Don Atce1: A Novel Mouse Cyclic Adenosine 3',5'-Monophosphate-Responsive Element-Binding Protein-Like Gene Exclusively Expressed in Postmeiotic Spermatids Endocrinology, May 1, 2002; 143(5): 1578 - 1588. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Osada, G. Watanabe, S. Kondo, M. Toyoda, Y. Sakaki, and T. Takeuchi Male Reproductive Defects Caused by Puromycin-Sensitive Aminopeptidase Deficiency in Mice Mol. Endocrinol., June 1, 2001; 15(6): 960 - 971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Matt Kim, J. T. McGaughy, R. Kent Bogle, and S. E. Ravnik Meiotic Expression of the Cyclin H/Cdk7 Complex in Male Germ Cells of the Mouse Biol Reprod, May 1, 2001; 64(5): 1400 - 1408. [Abstract] [Full Text]
|
|
|
|
 |
|
 T. Shinohara, K. E. Orwig, M. R. Avarbock, and R. L. Brinster From the Cover: Spermatogonial stem cell enrichment by multiparameter selection of mouse testis cells PNAS, July 18, 2000; 97(15): 8346 - 8351. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Pei Pituitary Tumor-transforming Gene Protein Associates with Ribosomal Protein S10 and a Novel Human Homologue of DnaJ in Testicular Cells J. Biol. Chem., January 29, 1999; 274(5): 3151 - 3158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ever, R. Steiner, S. Shalom, and J. Don Two Alternatively Spliced Meig1 Messenger RNA Species Are Differentially Expressed in the Somatic and in the Germ-Cell Compartments of the Testis Cell Growth Differ., January 1, 1999; 10(1): 19 - 26. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
