Complex Induction of Bovine Uterine Proteins by Interferon-Tau¹

^f Department of Animal Science and ^g School of Pharmacy, University of Wyoming, Laramie, Wyoming 82071

Interferon-tau (IFN-) is released by the conceptus and induces two uterine proteins during early pregnancy: ubiquitin cross-reactive protein (UCRP) and granulocyte chemotactic protein-2 (GCP-2). The present experiments were designed to determine whether detection (Western blot) of cytosolic UCRP and release of GCP-2 could be used to examine IFN- signal transduction in cultured endometrial explants and primary epithelial cells. Recombinant (r) type 1 IFNs (rboIFN- and rboIFN-; 5, 25, 100 nM) induced UCRP, but only rboIFN- induced GCP-2 in explant culture. Recombinant boIFN- and conceptus secretory proteins containing native IFN- induced UCRP and GCP-2 in cultured primary epithelial cells. All concentrations of rboIFN- (25, 50, 100 nM) induced UCRP, but only the highest concentration induced GCP-2 in cultured primary epithelial cells. Interestingly, phorbol ester (100, 500, 1000 ng/ml) induced GCP-2, but it had no effect on UCRP. Because type 1 IFNs induce UCRP, IFN- probably interacts with the janus kinase (Jak)-associated IFN- receptor to phosphorylate signal transducers and activators of transcription (STAT) and/or interferon regulatory factor-1 (IRF-1). However, IFN--specific induction of GCP-2 may involve a variant type 1 receptor subunit or activators of transcription that are associated with protein kinase C and the Jak/STAT/IRF-1 pathway.

¹ Supported in part by NIH (HD32475) and USDA (97-35203-4808) grants to T.R.H., and Equine Science Program, Northwest College, Powell, WY to V.A.D.

² Correspondence: Thomas R. Hansen, University of Wyoming, Reproductive Biology Program, Department of Animal Science, P.O. Box 3684, Laramie, WY 82071-3684. FAX: (307) 766-2355; thansen{at}uwyo.edu

³ Current address: Department of Animal Science, University of Missouri, Columbia, MO 65211.

⁴ Current address: Department of Animal Science, Texas A&; University, College Station, TX 77843.

⁵ Current address: Equine Science Program, Northwest College, Powell, WY 82435.

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