Biol Reprod Track the topics, authors and articles important to you
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Samathanam, C.A.
Right arrow Articles by Bondy, C.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Samathanam, C.A.
Right arrow Articles by Bondy, C.A.
Agricola
Right arrow Articles by Samathanam, C.A.
Right arrow Articles by Bondy, C.A.
Biology of Reproduction 59, 491-496 (1998)
©Copyright 1998 Society for the Study of Reproduction, Inc.

Fibroblast Growth Factors 1 and 2 in the Primate Uterus

C.A. Samathanama, O.O. Adesanyaa, J. Zhoua, J. Wanga, , and C.A. Bondya

a Section on Women's Health, Developmental Endocrinology Branch, NICHD, NIH, Bethesda, Maryland 20892

Fibroblast growth factors (FGF) 1 and 2 are paracrine effectors of proliferation and angiogenesis in many tissues. To elucidate potential roles for these growth factors in uterine plasticity, we used in situ hybridization histochemistry to identify the cellular sources of FGF-1 and -2 production, and immunohistochemistry to identify the cellular and extracellular deposition sites of the peptides in the primate uterus. To evaluate the effects of estradiol on uterine FGFs, uteri from ovariectomized rhesus monkeys treated with estradiol- or vehicle-containing pellets were investigated. FGF-1 and -2 mRNAs were both expressed in uterine epithelial and myometrial cells. Quantitative comparison of their mRNA levels using computerized grain counting showed no significant difference between estradiol- and vehicle-treated animals.

FGF-1 immunoreactivity was detected in scattered epithelial, vascular, and myometrial cells in the vehicle-treated animals but found to be significantly more intense and widespread in estradiol-treated animals. In both conditions, FGF-1 immunostaining was predominantly nuclear. FGF-2 immunoreactivity was concentrated extracellularly in the basal lamina of both glandular and surface epithelium and was abundant and diffusely distributed within myometrial and vascular cells in both cytoplasm and nucleus. There was no apparent difference in the pattern or intensity of FGF-2 immunostaining related to estradiol treatment. These data demonstrate that major uterine cell types synthesize both FGF-1 and -2, and that the two peptides are differentially localized in uterine cellular and extracellular compartments and differentially sensitive to regulation by estradiol.

1 Correspondence: Carolyn Bondy, Bldg. 10/10N262, 10 Center Dr., NIH, Bethesda, MD 20892. FAX: (301) 402-2922; bondyc{at}cc1.nichd.nih.gov




This article has been cited by other articles:


Home page
 J. Cell Sci.Home page
L. Cotton, G. M. Gibbs, L. G. Sanchez-Partida, J. R. Morrison, D. M. de Kretser, and M. K. O'Bryan
FGFR-1 signaling is involved in spermiogenesis and sperm capacitation
J. Cell Sci., January 1, 2006; 119(1): 75 - 84.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
S. M. Hyder and G. M. Stancel
Regulation of Angiogenic Growth Factors in the Female Reproductive Tract by Estrogens and Progestins
Mol. Endocrinol., June 1, 1999; 13(6): 806 - 811.
[Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Society for the Study of Reproduction.