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Biology of Reproduction 59, 621-625 (1998)
©Copyright 1998 Society for the Study of Reproduction, Inc.

Differential Expression of a Tumor Necrosis Factor Receptor-Related Transcript in Gestational Trophoblastic Diseases in Women1

Catherine I. Dumura, Nicolás P. Koritschonera, Alfredo Flurya, Graciela Panzetta-Dutaria, José L. Boccoa, , and Luis C. Patrito2,a

a Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Córdoba, Argentina

Gestational trophoblastic diseases comprise a group of interrelated neoplasms, including complete hydatidiform mole (CHM), persistent gestational trophoblastic tumor (GTT), and choriocarcinoma. To better define the molecular features of these diseases, a CHM cDNA library was constructed and a novel cDNA sequence, named CHMS-1, was isolated by differential screening. The CHMS-1 sequence showed a 62% homology with the tumor necrosis factor receptor (TNF-R2) cDNA, and its amino acid deduced sequence shared a high level of homology with the "death domain" region found in various proteins, including two members of the TNF receptor superfamily, the TNF-R1 and Fas.

We also determined the CHMS-1, TNF-R1, and TNF-R2 expression patterns among different CHM tissues and cell lines of trophoblastic (JEG-3) and nontrophoblastic (HeLa and COS-7) origin. Our results indicated that the CHMS-1 transcript is highly expressed in CHM in comparison with both normal early and term placenta and that it exhibits an expression profile identical to that of TNF-R1. Furthermore, the CHMS-1 transcript was undetectable in CHM-derived GTT and in the human choriocarcinoma-derived JEG-3 cells, suggesting that its expression is down-regulated in the malignant transformation of trophoblast. The presence of a potential "death domain" in CHMS-1, together with its high expression level in CHM, strongly suggests that the CHMS-1 gene encodes a protein that might be involved in tumor regression processes occurring at later stages of molar development.

1 This work was supported by grants from the Consejo Nacional de Investigaciones Científicas y Tecnológicas de Argentina (CONICET), the Consejo de Investigaciones Científicas y Tecnológicas de la Provincia de Córdoba (CONICOR) and the Secretaría de Ciencia y Técnica de la Universidad Nacional de Córdoba (SECyT). The nucleotide sequence reported in this work has been submitted to the GenBank Data Bank under the accession number: AF040257.

2 Correspondence: Luis C. Patrito, Departamento de Bioquímica Clínica, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, Agencia Postal N° 4. C.C. 61 (5000) Córdoba Argentina. FAX: 54 51–334174; lpatrito{at}fcq.uncor.edu






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Copyright © 1998 by the Society for the Study of Reproduction.