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a Department of Veterinary Anatomy and Public Health,
b Department of Animal Science, and
c Center for Animal Biotechnology, Institute of Biosciences and Technology, Texas A&M University, College Station, Texas 77843–4458
In porcine conceptuses, TGFßs 1, 2, and 3 mRNA expression was detected in trophectoderm, endoderm, embryonic ectoderm, and mesoderm. For the three TGFß isoforms examined, mRNA expression increased 2- to 4-fold in trophectoderm and endoderm between Days 10 and 14 of gestation. TGFß1 mRNA levels increased significantly in embryonic ectoderm, but not mesoderm, between Days 12 and 14 of gestation; during that same time, TGFß2 mRNA levels increased, but no change was detected in TGFß3 mRNA levels, in embryonic ectoderm and mesoderm.
Progressive increases in TGFß mRNA expression in conceptus trophectoderm, endoderm, ULE, UGs, and SS suggest important roles for these growth factors in porcine conceptus development during the peri-implantation period.
Spatial and temporal mRNA expression of beta transforming growth factors (TGFß1, TGFß2, and TGFß3) in porcine uterus and conceptuses was determined during the peri-implantation period (Days 10–14 of gestation). Northern blotting identified a major 3.5-kilobase (kb) and a minor 2.5-kb transcript for TGFß1 mRNA. TGFß2 transcripts were 6.2 kb, 5.4 kb, and 2.7 kb, and a single 3.5-kb transcript was detected for TGFß3. With semiquantitative in situ hybridization analysis, progressive increases were detected in TGFßs 1, 2, and 3 mRNA expression in uterine luminal epithelium (ULE), uterine glands (UGs), and underlying stroma (stroma spongiosum, SS) from Days 10 through 14 of gestation (p < 0.05). In myometrium, TGFß mRNA expression did not differ between Days 10 through 14 of gestation.
2 Correspondence. FAX: 409 847 8981; ljaeger{at}cvm.tamu.edu
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