| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Department of Veterinary Anatomy and Public Health,
b Department of Animal Science, and
c Center for Animal Biotechnology, Institute of Biosciences and Technology, Texas A&; University, College Station, Texas 77843–4458
Western blotting detected the presence of all three TGFß isoforms in uterine luminal flushings. The CCL64 cell TGFß bioassay detected bioactive TGFßs in uterine luminal flushings on Days 12, 13, and 14 of gestation. These results strongly indicate that uterine expression of TGFßs and their receptors is pregnancy specific and that bioactive TGFßs are present at the conceptus-maternal interface in the peri-implantation period in pigs. Thus TGFßs are likely to be involved in autocrine-paracrine interactions between the maternal uterus and the conceptus.
Porcine uterine tissues were collected from Days 10 to 14 of gestation (peri-implantation period) or corresponding days of the estrous cycle. Results indicated a marked increase in beta transforming growth factors (TGFß1, TGFß2, and TGFß3) and TGFß receptor (type I and type II) immunostaining in uterine luminal epithelium (ULE) between Days 10 and 14 of gestation, but there was no increase in ULE immunostaining on the corresponding days of the estrous cycle. Uterine glands and stroma were intensely immunopositive in pregnant gilts for TGFß isoforms and their receptors, but immunostaining was weak to undetectable in cycling gilts. No differences were detected in myometrium, in which immunostaining was moderate in both cycling and pregnant gilts. Additionally, TGFß2 and TGFß receptor (type I and type II) immunostaining was detected in uterine monocyte/macrophage-like cells.
2 Correspondence. FAX: 409 847 8981; ljaeger{at}cvm.tamu.edu
This article has been cited by other articles:
|
|
 |
|
  L. A. Jaeger, A. K. Spiegel, N. H. Ing, G. A. Johnson, F. W. Bazer, and R. C. Burghardt Functional Effects of Transforming Growth Factor {beta} on Adhesive Properties of Porcine Trophectoderm Endocrinology, September 1, 2005; 146(9): 3933 - 3942. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E E-D A Moussad, M A E Rageh, A K Wilson, R D Geisert, and D R Brigstock Temporal and spatial expression of connective tissue growth factor (CCN2; CTGF) and transforming growth factor {beta} type 1 (TGF-{beta}1) at the utero-placental interface during early pregnancy in the pig Mol. Pathol., June 1, 2002; 55(3): 186 - 192. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. F.C. Chow, K.-F. Lee, S. T.H. Chan, and W. S.B. Yeung Quantification of transforming growth factor {beta}1 (TGF{beta}1) mRNA expression in mouse preimplantation embryos and determination of TGF{beta} receptor (type I and type II) expression in mouse embryos and reproductive tract Mol. Hum. Reprod., November 1, 2001; 7(11): 1047 - 1056. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Ka, L. A. Jaeger, G. A. Johnson, T. E. Spencer, and F. W. Bazer Keratinocyte Growth Factor Is Up-Regulated by Estrogen in the Porcine Uterine Endometrium and Functions in Trophectoderm Cell Proliferation and Differentiation Endocrinology, June 1, 2001; 142(6): 2303 - 2310. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. R. Brigstock The Connective Tissue Growth Factor/Cysteine- Rich 61/Nephroblastoma Overexpressed (CCN) Family Endocr. Rev., April 1, 1999; 20(2): 189 - 206. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Gupta,, N. H. Ing,, F. W. Bazer,, L. S. Bustamante,, and L. A. Jaeger Beta Transforming Growth Factors (TGFß) at the Porcine Conceptus-Maternal Interface. Part I: Expression of TGFß1, TGFß2, and TGFß3 Messenger Ribonucleic Acids Biol Reprod, October 1, 1998; 59(4): 905 - 910. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
