Biol Reprod Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Aitken, R. J.
Right arrow Articles by Irvine, D. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Aitken, R. J.
Right arrow Articles by Irvine, D. S.
Agricola
Right arrow Articles by Aitken, R. J.
Right arrow Articles by Irvine, D. S.
Biology of Reproduction 59, 1037-1046 (1998)
©Copyright 1998 Society for the Study of Reproduction, Inc.

Relative Impact of Oxidative Stress on the Functional Competence and Genomic Integrity of Human Spermatozoa1

R. John Aitken2,a, Emma Gordona, Diana Harkissa, Jeremy P. Twigga, Philip Milnea, Zoë Jenningsa, , and D. Stewart Irvinea

a MRC Reproductive Biology Unit, Edinburgh EH3 9EW, United Kingdom

Reactive oxygen metabolites are known to disrupt sperm-oocyte fusion, sperm movement, and DNA integrity; however, the relative sensitivities of these elements to oxidative stress are unknown. In this study these factors were assessed in human spermatozoa exposed to increasing levels of oxidative stress achieved through the stimulation of endogenous oxidant generation with NADPH or direct exposure to hydrogen peroxide. At low levels of oxidative stress, DNA fragmentation was significantly reduced while the rates of sperm-oocyte fusion were significantly enhanced. As the level of oxidative stress increased, the spermatozoa exhibited significantly elevated levels of DNA damage (p < 0.001) and yet continued to express an enhanced capacity for sperm-oocyte fusion. At the highest levels of oxidative stress, extremely high rates of DNA fragmentation were observed but the spermatozoa exhibited a parallel loss in their capacities for movement and oocyte fusion. These studies emphasize how redox mechanisms can either enhance or disrupt the functional and genomic integrity of human spermatozoa depending on the intensity of the oxidative stimulus. Because these qualities are affected at different rates, spermatozoa exhibiting significant DNA damage are still capable of fertilizing the oocyte. These results may have long-term implications for the safety of assisted conception procedures in cases associated with oxidative stress.

1 This study was conducted under the auspices of a license from the Human Fertilization and Embryology Society of the United Kingdom (R0056).

2 Correspondence: R. John Aitken, MRC Reproductive Biology Unit, 37 Chalmers Street, Edinburgh EH3 9EW, UK. FAX: 00 44 131 228 5571; r.j.aitken{at}ed-rbu.mrc.ac.uk




This article has been cited by other articles:


Home page
 Hum Reprod UpdateHome page
K. Tremellen
Oxidative stress and male infertility--a clinical perspective
Hum. Reprod. Update, May 1, 2008; 14(3): 243 - 258.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. Tateno and Y. Kamiguchi
Evaluation of Chromosomal Risk Following Intracytoplasmic Sperm Injection in the Mouse
Biol Reprod, August 1, 2007; 77(2): 336 - 342.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
S. E M Lewis
Is sperm evaluation useful in predicting human fertility?
Reproduction, July 1, 2007; 134(1): 31 - 40.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
I.M. Agbaje, D.A. Rogers, C.M. McVicar, N. McClure, A.B. Atkinson, C. Mallidis, and S.E.M. Lewis
Insulin dependant diabetes mellitus: implications for male reproductive function
Hum. Reprod., July 1, 2007; 22(7): 1871 - 1877.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
N. H. Aneck-Hahn, G. W. Schulenburg, M. S. Bornman, P. Farias, and C. De Jager
Impaired Semen Quality Associated With Environmental DDT Exposure in Young Men Living in a Malaria Area in the Limpopo Province, South Africa
J Androl, May 1, 2007; 28(3): 423 - 434.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
S. Wang, G. Wang, B. E. Barton, T. F. Murphy, and H. F. S. Huang
Beneficial Effects of Vitamin E in Sperm Functions in the Rat After Spinal Cord Injury
J Androl, March 1, 2007; 28(2): 334 - 341.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
L. Muriel, V. Goyanes, E. Segrelles, J. Gosalvez, J. G. Alvarez, and J. L. Fernandez
Increased Aneuploidy Rate in Sperm With Fragmented DNA as Determined by the Sperm Chromatin Dispersion (SCD) Test and FISH Analysis
J Androl, January 1, 2007; 28(1): 38 - 49.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
T. R. Kumar and Muralidhara
Induction of Oxidative Stress by Organic Hydroperoxides in Testis and Epididymal Sperm of Rats In Vivo
J Androl, January 1, 2007; 28(1): 77 - 85.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
N. Torregrosa, D. Dominguez-Fandos, M. I. Camejo, C. R. Shirley, M. L. Meistrich, J. L. Ballesca, and R. Oliva
Protamine 2 precursors, protamine 1/protamine 2 ratio, DNA integrity and other sperm parameters in infertile patients
Hum. Reprod., August 1, 2006; 21(8): 2084 - 2089.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Liu, H. Lin, S. Ye, Q.-y. Liu, Z. Meng, C.-m. Zhang, Y. Xia, E. Margoliash, Z. Rao, and X.-j. Liu
Remarkably high activities of testicular cytochrome c in destroying reactive oxygen species and in triggering apoptosis
PNAS, June 13, 2006; 103(24): 8965 - 8970.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Ambrosini, G. Zolese, S. Ambrosi, L. Ragni, L. Tiano, G. Littarru, E. Bertoli, F. Mantero, M. Boscaro, and G. Balercia
Oleoylethanolamide Protects Human Sperm Cells from Oxidation Stress: Studies on Cases of Idiopathic Infertility
Biol Reprod, April 1, 2006; 74(4): 659 - 665.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
C. De Jager, P. Farias, A. Barraza-Villarreal, M. H. Avila, P. Ayotte, E. Dewailly, C. Dombrowski, F. Rousseau, V. D. Sanchez, and J. L. Bailey
Reduced Seminal Parameters Associated With Environmental DDT Exposure and p,p'-DDE Concentrations in Men in Chiapas, Mexico: A Cross-Sectional Study
J Androl, January 1, 2006; 27(1): 16 - 27.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
K. R. Chohan, J. T. Griffin, M. Lafromboise, C. J. De Jonge, and D. T. Carrell
Comparison of Chromatin Assays for DNA Fragmentation Evaluation in Human Sperm
J Androl, January 1, 2006; 27(1): 53 - 59.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
J. R. Starr, C. Chen, D. R. Doody, L. Hsu, S. Ricks, N. S. Weiss, and S. M. Schwartz
Risk of Testicular Germ Cell Cancer in Relation to Variation in Maternal and Offspring Cytochrome P450 Genes Involved in Catechol Estrogen Metabolism
Cancer Epidemiol. Biomarkers Prev., September 1, 2005; 14(9): 2183 - 2190.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. G. Alvarez
'Efficient treatment of infertility due to sperm DNA damage by ICSI with testicular sperm'
Hum. Reprod., July 1, 2005; 20(7): 2031 - 2032.
[Full Text] [PDF]

Home page
 ReproductionHome page
S. Banks, S. A King, D S. Irvine, and P. T K Saunders
Impact of a mild scrotal heat stress on DNA integrity in murine spermatozoa
Reproduction, April 1, 2005; 129(4): 505 - 514.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S.L. Taylor, S.L. Weng, P. Fox, E.H. Duran, M.S. Morshedi, S. Oehninger, and S.J. Beebe
Somatic cell apoptosis markers and pathways in human ejaculated sperm: potential utility as indicators of sperm quality
Mol. Hum. Reprod., November 1, 2004; 10(11): 825 - 834.
[Abstract] [Full Text] [PDF]

Home page
 Hum Reprod UpdateHome page
W.C.L. Ford
Regulation of sperm function by reactive oxygen species
Hum. Reprod. Update, September 1, 2004; 10(5): 387 - 399.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
R. J. Aitken, M. A. Baker, and M. O'Bryan
Shedding Light on Chemiluminescence: The Application of Chemiluminescence in Diagnostic Andrology
J Androl, July 1, 2004; 25(4): 455 - 465.
[Full Text] [PDF]

Home page
 Hum ReprodHome page
S.E.M. Lewis, M. O'Connell, M. Stevenson, L. Thompson-Cree, and N. McClure
An algorithm to predict pregnancy in assisted reproduction
Hum. Reprod., June 1, 2004; 19(6): 1385 - 1394.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. B. DuTeaux, T. Berger, R. A. Hess, B. L. Sartini, and M. G. Miller
Male Reproductive Toxicity of Trichloroethylene: Sperm Protein Oxidation and Decreased Fertilizing Ability
Biol Reprod, May 1, 2004; 70(5): 1518 - 1526.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. Marcon and G. Boissonneault
Transient DNA Strand Breaks During Mouse and Human Spermiogenesis:New Insights in Stage Specificity and Link to Chromatin Remodeling
Biol Reprod, April 1, 2004; 70(4): 910 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. H. Moustafa, R. K. Sharma, J. Thornton, E. Mascha, M. A. Abdel-Hafez, A. J. Thomas, and A. Agarwal
Relationship between ROS production, apoptosis and DNA denaturation in spermatozoa from patients examined for infertility
Hum. Reprod., January 1, 2004; 19(1): 129 - 138.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
J. Baumber, B. A. Ball, J. J. Linfor, and S. A. Meyers
Reactive Oxygen Species and Cryopreservation Promote DNA Fragmentation in Equine Spermatozoa
J Androl, July 1, 2003; 24(4): 621 - 628.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
M. Muratori, M. Maggi, S. Spinelli, E. Filimberti, G. Forti, and E. Baldi
Spontaneous DNA Fragmentation in Swim-Up Selected Human Spermatozoa During Long Term Incubation
J Androl, March 1, 2003; 24(2): 253 - 262.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S.-L. Weng, S. L. Taylor, M. Morshedi, A. Schuffner, E.H. Duran, S. Beebe, and S. Oehninger
Caspase activity and apoptotic markers in ejaculated human sperm
Mol. Hum. Reprod., November 1, 2002; 8(11): 984 - 991.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Oehninger and R. G. Gosden
Should ICSI be the treatment of choice for all cases of in-vitro conception?: No, not in light of the scientific data
Hum. Reprod., September 1, 2002; 17(9): 2237 - 2242.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
D. Perera, A. Pizzey, A. Campbell, M. Katz, J. Porter, M. Petrou, D.S. Irvine, and R. Chatterjee
Sperm DNA damage in potentially fertile homozygous {beta}-thalassaemia patients with iron overload
Hum. Reprod., July 1, 2002; 17(7): 1820 - 1825.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
I.D. Morris, S. Ilott, L. Dixon, and D.R. Brison
The spectrum of DNA damage in human sperm assessed by single cell gel electrophoresis (Comet assay) and its relationship to fertilization and embryo development
Hum. Reprod., April 1, 2002; 17(4): 990 - 998.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Aguilar-Mahecha, B. F. Hales, and B. Robaire
Chronic Cyclophosphamide Treatment Alters the Expression of Stress Response Genes in Rat Male Germ Cells
Biol Reprod, April 1, 2002; 66(4): 1024 - 1032.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Anzar, L. He, M. M. Buhr, T. G. Kroetsch, and K. P. Pauls
Sperm Apoptosis in Fresh and Cryopreserved Bull Semen Detected by Flow Cytometry and Its Relationship with Fertility
Biol Reprod, February 1, 2002; 66(2): 354 - 360.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
J. Tesarik, E. Greco, and C. Mendoza
Assisted reproduction with in-vitro-cultured testicular spermatozoa in cases of severe germ cell apoptosis: a pilot study
Hum. Reprod., December 1, 2001; 16(12): 2640 - 2645.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Ollero, E. Gil-Guzman, M. C. Lopez, R. K. Sharma, A. Agarwal, K. Larson, D. Evenson, A. J. Thomas Jr, and J. G. Alvarez
Characterization of subsets of human spermatozoa at different stages of maturation: implications in the diagnosis and treatment of male infertility
Hum. Reprod., September 1, 2001; 16(9): 1912 - 1921.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
E.V. Younglai, D. Holt, P. Brown, A. Jurisicova, and R.F. Casper
Sperm swim-up techniques and DNA fragmentation
Hum. Reprod., September 1, 2001; 16(9): 1950 - 1953.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Emiliani, M. Van den Bergh, A.-S. Vannin, J. Biramane, M. Verdoodt, and Y. Englert
Evidence of reduced single-stranded testicular sperm DNA from obstructive azoospermic men after 3 days of in-vitro culture
Hum. Reprod., June 1, 2001; 16(6): 1200 - 1203.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
S. C. Richer and W.C.L. Ford
A critical investigation of NADPH oxidase activity in human spermatozoa
Mol. Hum. Reprod., March 1, 2001; 7(3): 237 - 244.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
S. Banerjee, S. Lamond, A. McMahon, S. Campbell, and G. Nargund
Does blastocyst culture eliminate paternal chromosomal defects and select good embryos?: Inheritance of an abnormal paternal genome following ICSI
Hum. Reprod., December 1, 2000; 15(12): 2455 - 2459.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
M. Sergerie, S. Ouhilal, F. Bissonnette, J. Brodeur, and G. Bleau
Lack of association between smoking and DNA fragmentation in the spermatozoa of normal men
Hum. Reprod., June 1, 2000; 15(6): 1314 - 1321.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
G. Barroso, M. Morshedi, and S. Oehninger
Analysis of DNA fragmentation, plasma membrane translocation of phosphatidylserine and oxidative stress in human spermatozoa
Hum. Reprod., June 1, 2000; 15(6): 1338 - 1344.
[Abstract] [Full Text] [PDF]

Home page
 Hum ReprodHome page
R. Chatterjee, G.A. Haines, D.M.D. Perera, A. Goldstone, and I.D. Morris
Testicular and sperm DNA damage after treatment with fludarabine for chronic lymphocytic leukaemia
Hum. Reprod., April 1, 2000; 15(4): 762 - 766.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
D. M. de Kretser and H. W. G. Baker
Infertility in Men: Recent Advances and Continuing Controversies
J. Clin. Endocrinol. Metab., October 1, 1999; 84(10): 3443 - 3450.
[Full Text] [PDF]

Home page
 IOVSHome page
H. Chen, J. Ray, V. Scarpino, G. M. Acland, G. D. Aguirre, and R. E. Anderson
Synthesis and Release of Docosahexaenoic Acid by the RPE Cells of prcd-Affected Dogs
Invest. Ophthalmol. Vis. Sci., September 1, 1999; 40(10): 2418 - 2422.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Society for the Study of Reproduction.