| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Ottawa Regional Cancer Centre, and Departments of Medicine, Cellular and Molecular Medicine, and Obstetrics&Gynecology, University of Ottawa, Ottawa, Ontario, Canada K1H 8L6
Mouse oocytes secrete a factor(s) that inhibits progesterone and enhances estradiol production by granulosa cells. This study determined the ability of mouse oocytes to secrete this steroid-regulating factor during oocyte growth and the ability of granulosa cells to respond to the factor during follicular development. Oocyte-granulosa cell complexes from preantral and antral follicles were oocytectomized (OOX; oocytes microsurgically removed) and cultured for up to 48 h with FSH (150 ng/ml) and testosterone (500 nM). At all stages of development examined, OOX complexes produced more progesterone than did intact complexes, from 1.45-fold for early growing follicles to 23-fold for complexes from antral follicles. Significant estradiol production was restricted to intact complexes from late antral follicles. Progesterone accumulation by OOX complexes cocultured with oocytes was inhibited by all stages of oocytes examined, with maximal inhibition by fully grown oocytes. Ovulated complexes produced large quantities of progesterone, even though oocytes secreted progesterone-inhibitory factor, because of a desensitization of cumulus cells to the factor during their terminal differentiation. Even in the presence of abundant pregnenolone, OOX complexes showed reduced ability to produce and/or accumulate progesterone in the presence of oocytes, suggesting that the oocyte-secreted factor, either directly or indirectly, regulates the activity of 3ß-hydroxysteroid dehydrogenase and/or progesterone metabolism. These results demonstrate that oocytes secrete a factor with steroid-regulating activity in increasing amounts and/or potency during follicular development, but responsiveness of cumulus cells to this factor declines during luteinization.
This article has been cited by other articles:
|
|
 |
|
  J. E. Swain and T. B. Pool ART failure: oocyte contributions to unsuccessful fertilization Hum. Reprod. Update, September 1, 2008; 14(5): 431 - 446. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. B. Gilchrist, M. Lane, and J. G. Thompson Oocyte-secreted factors: regulators of cumulus cell function and oocyte quality Hum. Reprod. Update, March 1, 2008; 14(2): 159 - 177. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. C. Teilmann, C. A. Clement, J. Thorup, A. G. Byskov, and S. T. Christensen Expression and localization of the progesterone receptor in mouse and human reproductive organs J. Endocrinol., December 1, 2006; 191(3): 525 - 535. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-Q. Su, M. Nyegaard, M. T. Overgaard, J. Qiao, and L. C. Giudice Participation of Mitogen-Activated Protein Kinase in Luteinizing Hormone-Induced Differential Regulation of Steroidogenesis and Steroidogenic Gene Expression in Mural and Cumulus Granulosa Cells of Mouse Preovulatory Follicles Biol Reprod, December 1, 2006; 75(6): 859 - 867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Guigon and S. Magre Contribution of Germ Cells to the Differentiation and Maturation of the Ovary: Insights from Models of Germ Cell Depletion Biol Reprod, March 1, 2006; 74(3): 450 - 458. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Roberts, J. Stark, A. Iatropoulou, D. L. Becker, S. Franks, and K. Hardy Energy Substrate Metabolism of Mouse Cumulus-Oocyte Complexes: Response to Follicle-Stimulating Hormone Is Mediated by the Phosphatidylinositol 3-Kinase Pathway and Is Associated with Oocyte Maturation Biol Reprod, July 1, 2004; 71(1): 199 - 209. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Glister, N. P. Groome, and P. G. Knight Oocyte-Mediated Suppression of Follicle-Stimulating Hormone- and Insulin-Like Growth Factor-Induced Secretion of Steroids and Inhibin-Related Proteins by Bovine Granulosa Cells In Vitro: Possible Role of Transforming Growth Factor {alpha} Biol Reprod, March 1, 2003; 68(3): 758 - 765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rothchild The Yolkless Egg and the Evolution of Eutherian Viviparity Biol Reprod, February 1, 2003; 68(2): 337 - 357. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.L. Luborsky, P. Thiruppathi, B. Rivnay, R. Roussev, C. Coulam, and E. Radwanska Evidence for different aetiologies of low estradiol response to FSH: age-related accelerated luteinization of follicles or presence of ovarian autoantibodies Hum. Reprod., October 1, 2002; 17(10): 2641 - 2649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D'Alessandris, R. Canipari, M. Di Giacomo, O. Epifano, A. Camaioni, G. Siracusa, and A. Salustri Control of Mouse Cumulus Cell-Oocyte Complex Integrity before and after Ovulation: Plasminogen Activator Synthesis and Matrix Degradation Endocrinology, July 1, 2001; 142(7): 3033 - 3040. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. D. Murphy Models of Luteinization Biol Reprod, July 1, 2000; 63(1): 2 - 11. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. A. McGee and A. J. W. Hsueh Initial and Cyclic Recruitment of Ovarian Follicles Endocr. Rev., April 1, 2000; 21(2): 200 - 214. [Abstract] [Full Text]
|
|
|
|
|
|
A. Dhawan, M.-C. Leveille, and B.C. Vanderhyden Inhibition by human embryos of mouse granulosa cell progesterone production: development of a sensitive bioassay Hum. Reprod., April 1, 2000; 15(4): 917 - 924. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. J. Eppig and K. Wigglesworth Development of Mouse and Rat Oocytes in Chimeric Reaggregated Ovaries after Interspecific Exchange of Somatic and Germ Cell Components Biol Reprod, April 1, 2000; 63(4): 1014 - 1023. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. Farioli–Vecchioli, S. Raes, M. Espeel, F. Roels, and K. D'Herde Inverse Expression of Peroxisomes and Connexin-43 in the Granulosa Cells of the Quail Follicle J. Histochem. Cytochem., February 1, 2000; 48(2): 167 - 178. [Abstract] [Full Text]
|
|
|
|
|
|
J. F. Couse, D. O. Bunch, J. Lindzey, D. W. Schomberg, and K. S. Korach Prevention of the Polycystic Ovarian Phenotype and Characterization of Ovulatory Capacity in the Estrogen Receptor-{alpha} Knockout Mouse Endocrinology, December 1, 1999; 140(12): 5855 - 5865. [Abstract] [Full Text]
|
|
|
|
|
|
J. F. Couse and K. S. Korach Estrogen Receptor Null Mice: What Have We Learned and Where Will They Lead Us? Endocr. Rev., June 1, 1999; 20(3): 358 - 417. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
