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a Department of Physiology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901-6512
b School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104
c Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil 31270-901 CP 486
Development of spermatogonial transplants was studied by using 5- to 6-wk-old histocompatible mice as cell donors and sterile (W-locus) mice as recipients. Groups of animals transplanted with germ cell suspensions were killed at 10 min, 9 h, 24 h, 1 wk, 1 mo, 2 mo, and 3 mo along with age-matched "start" and "end" W-locus controls. Weight of testes increased significantly at 24 h through 3 mo after germ cell transplantation, suggesting that the infused cells quickly stimulated organ function. Small clones of young spermatocytes were evident at 1 mo and sperm at 2 mo. The percentage of tubular profiles containing active spermatogenesis originating from spermatogonia increased with time (0.8% at 1 mo, 8.9% at 2 mo, and 28.2% at 3 mo). Most transplanted germ cells were eliminated from the seminiferous epithelium through phagocytosis by Sertoli cells that occurred primarily before 1 wk, although some pachytene cells were able to proceed through meiosis by 1 wk. A variety of abnormal features are described that characterize developing spermatogenesis in the transplanted testis. Spermatogenesis improved quantitatively and qualitatively with time although released sperm were frequently engulfed by intratubular macrophages and Sertoli cells. A quantitative analysis of spermatogenesis from transplanted germ cells will serve as a basis for improving spermatogonial transplant efficiency.
Financial support from the National Institutes of Health (HD 36504 and HD35494), USDA/NRI Competitive Grants Program, Commonwealth and General Assembly of Pennsylvania, and the Robert J. Kleberg Jr. and Helen C. Kleberg Foundation as well as support of the Brazilian Research Foundation, CNPq, for Ms. Gleydes Parreira and FAPEMIG for Luiz França is gratefully acknowledged.
2 Correspondence. FAX: 618 453 1517; lrussell{at}som.siu.edu
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