Biol Reprod
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by França, L. R.
Right arrow Articles by Russell, L. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by França, L. R.
Right arrow Articles by Russell, L. D.
Agricola
Right arrow Articles by França, L. R.
Right arrow Articles by Russell, L. D.
Biology of Reproduction 59, 1371-1377 (1998)
©Copyright 1998 Society for the Study of Reproduction, Inc.

Germ Cell Genotype Controls Cell Cycle during Spermatogenesis in the Rat1

Luiz R. Françaa, Takehiko Ogawab, Mary R. Avarbockb, Ralph L. Brinsterb, and Lonnie D. Russell2,c

a Department of Morphology, Institute of Biological Sciences, Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, Brazil 31270-901 CP 486 b School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104 c Department of Physiology, Southern Illinois University School of Medicine, Carbondale, Illinois 62901-6512

Spermatogenesis is one of the most productive self-renewing systems in the body: on the order of 107 spermatozoa are produced daily per gram of testis tissue. In each mammalian species, the time required for completion of the process is unique and unalterable. Because the process is supported by somatic Sertoli cells, it has generally been thought that cell-cell interaction between germ and Sertoli cells controls the duration of cell cycles and cellular organization. We have used the newly developed technique of spermatogonial transplantation to examine which cell type(s) determines the rate at which germ cells proceed through spermatogenesis. Rat germ cells were transplanted into a mouse testis, and the mouse was killed 12.9–13 days after administration of a single dose of [3H]thymidine. The most advanced rat cell type labeled was the pachytene spermatocyte at stages VI–VIII of the spermatogenic cycle. In animals given only rat cells, some endogenous spermatogenesis of the mouse recovered. The most advanced labeled mouse cell types in recipients killed 12.9–13 days after administration of a single dose of [3H]thymidine were meiotic cells or young spermatids, which is consistent with a spermatogenic cycle length comparable to the 8.6 days reported for the mouse. The same results were obtained if a mixture of rat and mouse cells were transplanted. There existed two separate timing regimens for germ cell development in the recipient mouse testis; one of rat and one of mouse duration. Rat germ cells that were supported by mouse Sertoli cells always differentiated with cell cycle timing characteristic of the rat and generated the spermatogenic structural pattern of the rat, demonstrating that the cell differentiation process of spermatogenesis is regulated by germ cells alone.

1 Editor's Note: The research results described in this paper and its companion (Biol Reprod 1998; 59:1360–1370) were presented, in part, as a State-of-the-Art Lecture at the 31st Annual Meeting of the Society for the Study of Reproduction held at Texas A&M University, College Station, Texas, August 8–11, 1998. Both papers represent original research and have undergone stringent peer review.Support of the Brazilian Research Foundation (CNPq) and the State Research Foundation of Minas Gerais (FAPEMIG) are gratefully acknowledged. Financial support was also received from the National Institutes of Health (HD 36504 and HD35494), USDA/NRI Competitive Grants Program, Commonwealth and General Assembly of Pennsylvania, and the Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation.

2 Correspondence. FAX: 618 453 1517; lrussell{at}som.siu.edu




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
F. F.L Almeida, C. Kristoffersen, G. L. Taranger, and R. W Schulz
Spermatogenesis in Atlantic Cod (Gadus morhua): A Novel Model of Cystic Germ Cell Development
Biol Reprod, January 1, 2008; 78(1): 27 - 34.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
R. Parapanov, S. Nussle, and P. Vogel
Cycle Length of Spermatogenesis in Shrews (Mammalia: Soricidae) with High and Low Metabolic Rates and Different Mating Systems
Biol Reprod, May 1, 2007; 76(5): 833 - 840.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
F. F.L. Almeida, M. C. Leal, and L. R. Franca
Testis Morphometry, Duration of Spermatogenesis, and Spermatogenic Efficiency in the Wild Boar (Sus scrofa scrofa)
Biol Reprod, November 1, 2006; 75(5): 792 - 799.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Shinohara, M. Kato, M. Takehashi, J. Lee, S. Chuma, N. Nakatsuji, M. Kanatsu-Shinohara, and M. Hirabayashi
Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination
PNAS, September 12, 2006; 103(37): 13624 - 13628.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
W. Zeng, G. F. Avelar, R. Rathi, L. R. Franca, and I. Dobrinski
The Length of the Spermatogenic Cycle Is Conserved in Porcine and Ovine Testis Xenografts
J Androl, July 1, 2006; 27(4): 527 - 533.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. C. Leal and L. R. Franca
The Seminiferous Epithelium Cycle Length in the Black Tufted-Ear Marmoset (Callithrix penicillata) Is Similar to Humans
Biol Reprod, April 1, 2006; 74(4): 616 - 624.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. Meng, R. W. Holdcraft, J. E. Shima, M. D. Griswold, and R. E. Braun
Androgens regulate the permeability of the blood-testis barrier
PNAS, November 15, 2005; 102(46): 16696 - 16700.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
H. Khaira, D. McLean, D. A. Ohl, and G. D. Smith
Spermatogonial Stem Cell Isolation, Storage, and Transplantation
J Androl, July 1, 2005; 26(4): 442 - 450.
[Full Text] [PDF]

Home page
 J AndrolHome page
T. M. Segatelli, L. R. Franca, P. F. F. Pinheiro, C. C. D. Alemida, M. Martinez, and F. E. Martinez
Spermatogenic Cycle Length and Spermatogenic Efficiency in the Gerbil (Meriones unguiculatus)
J Androl, November 1, 2004; 25(6): 872 - 880.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
K. Neubauer, K. Jewgenow, S. Blottner, D. E. Wildt, and B. S. Pukazhenthi
Quantity Rather Than Quality in Teratospermic Males: A Histomorphometric and Flow Cytometric Evaluation of Spermatogenesis in the Domestic Cat (Felis catus)
Biol Reprod, November 1, 2004; 71(5): 1517 - 1524.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. C. Nagano
Homing Efficiency and Proliferation Kinetics of Male Germ Line Stem Cells Following Transplantation in Mice
Biol Reprod, August 1, 2003; 69(2): 701 - 707.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
L. R. Franca and C. L. Godinho
Testis Morphometry, Seminiferous Epithelium Cycle Length, and Daily Sperm Production in Domestic Cats (Felis catus)
Biol Reprod, May 1, 2003; 68(5): 1554 - 1561.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
Z. Zhang, M. B. Renfree, and R. V. Short
Successful Intra- and Interspecific Male Germ Cell Transplantation in the Rat
Biol Reprod, March 1, 2003; 68(3): 961 - 967.
[Abstract] [Full Text] [PDF]

Home page
 J AndrolHome page
J. Blanco-Rodriguez
DNA Replication and Germ Cell Apoptosis During Spermatogenesis in the Cat
J Androl, July 1, 2002; 23(4): 484 - 490.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
E. S. Neves, H. Chiarini-Garcia, and L. R. Franca
Comparative Testis Morphometry and Seminiferous Epithelium Cycle Length in Donkeys and Mules
Biol Reprod, July 1, 2002; 67(1): 247 - 255.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
R. L. Brinster
Germline Stem Cell Transplantation and Transgenesis
Science, June 21, 2002; 296(5576): 2174 - 2176.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
D. J. McLean, L. D. Russell, and M. D. Griswold
Biological Activity and Enrichment of Spermatogonial Stem Cells in Vitamin A-Deficient and Hyperthermia-Exposed Testes from Mice Based on Colonization Following Germ Cell Transplantation
Biol Reprod, May 1, 2002; 66(5): 1374 - 1379.
[Abstract] [Full Text]

Home page
 Recent Prog Horm ResHome page
E. M. Eddy
Male Germ Cell Gene Expression
Recent Prog. Horm. Res., January 1, 2002; 57(1): 103 - 128.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. Honaramooz, S. O. Megee, and I. Dobrinski
Germ Cell Transplantation in Pigs
Biol Reprod, January 1, 2002; 66(1): 21 - 28.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
T. M. Plant and G. R. Marshall
The Functional Significance of FSH in Spermatogenesis and the Control of Its Secretion in Male Primates
Endocr. Rev., December 1, 2001; 22(6): 764 - 786.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
L. O'Donnell, K. M. Robertson, M. E. Jones, and E. R. Simpson
Estrogen and Spermatogenesis
Endocr. Rev., June 1, 2001; 22(3): 289 - 318.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
I. Huhtaniemi and A. Bartke
Perspective: Male Reproduction
Endocrinology, June 1, 2001; 142(6): 2178 - 2183.
[Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
K. Boekelheide, S. L. Fleming, K. J. Johnson, S. R. Patel, and H. A. Schoenfeld
Role of Sertoli Cells in Injury-Associated Testicular Germ Cell Apoptosis
Experimental Biology and Medicine, November 1, 2000; 225(2): 105 - 115.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. C. Weglarz and E. P. Sandgren
Timing of hepatocyte entry into DNA synthesis after partial hepatectomy is cell autonomous
PNAS, October 23, 2000; (2000) 220430497.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
J. J. Eppig and K. Wigglesworth
Development of Mouse and Rat Oocytes in Chimeric Reaggregated Ovaries after Interspecific Exchange of Somatic and Germ Cell Components
Biol Reprod, April 1, 2000; 63(4): 1014 - 1023.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
M. D. Griswold
Editorial: What Can Spermatogonial Transplants Teach Us about Male Reproductive Biology?
Endocrinology, March 1, 2000; 141(3): 857 - 858.
[Full Text] [PDF]

Home page
 Biol. Reprod.Home page
I. Dobrinski, M. R. Avarbock, and R. L. Brinster
Transplantation of Germ Cells from Rabbits and Dogs Into Mouse Testes
Biol Reprod, November 1, 1999; 61(5): 1331 - 1339.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
T. Ogawa, I. Dobrinski, M. R. Avarbock, and R. L. Brinster
Xenogeneic Spermatogenesis Following Transplantation of Hamster Germ Cells to Mouse Testes
Biol Reprod, February 1, 1999; 60(2): 515 - 521.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
G. G. Parreira, T. Ogawa, M. R. Avarbock, L. R. França, R. L. Brinster, and L. D. Russell
Development of Germ Cell Transplants in Mice
Biol Reprod, December 1, 1998; 59(6): 1360 - 1370.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
T. C. Weglarz and E. P. Sandgren
Timing of hepatocyte entry into DNA synthesis after partial hepatectomy is cell autonomous
PNAS, November 7, 2000; 97(23): 12595 - 12600.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Society for the Study of Reproduction.