Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow My Folders
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by STRAUSS, J. F.
Right arrow Articles by STAMBAUGH, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by STRAUSS, J. F., III
Right arrow Articles by STAMBAUGH, R.
Agricola
Right arrow Articles by STRAUSS, J. F.
Right arrow Articles by STAMBAUGH, R.

Biology of Reproduction, Vol 6, 78-86, Copyright © 1972 by Society for the Study of Reproduction

20agr-Hydroxysteroid Dehydrogenase Activity in the Rabbit Ovary

J. F. STRAUSS III 1, B. FOLEY 1, , and R. STAMBAUGH 1

1 Division of Reproductive Biology, Department of Obstetrics and Gynecology, University of Pennsylvania School of Medicine, Philadelphia, Pennsylvania 19104


A spectrophotometric assay for rabbit ovarian 20agr-hydroxysteroid dehydrogenase (20agr-OH-SDH) is described. In whole ovaries 20agr-OH-SDH activity was found to be high during estrus. Following a 100 IU dose of HCG, 20agr-OH-SDH activity in whole ovaries declined to frac16 estrous levels by Day 3 of pseudopregnancy, but increased progressively, approaching estrous levels between Days 15 and 21 of pseudopregnancy. 20agr-OH-SDH activity in separated interstitial tissue increased 3.8-fold from Days 4-20, while the activity in corpora lutea (CL) was low and constant between Days 4 and 11, but rose 4-fold between Days 15 and 20. Direct addition of HCG to the extracts had no effect on 20agr-OH-SDH activity, but estriol was found to inhibit the enzyme. In contrast, glucose-6-phosphate dehydrogenase (G-6-PDH) activity in CL declined approximately 40% between Days 4 and 20 of pseudopregnancy. The results indicate that increasing 20agr-OH-SDH activity is a specific and sensitive index of luteolysis in the rabbit.

Submitted on April 6, 1971




This article has been cited by other articles:


Home page
 J. Histochem. Cytochem.Home page
G. Pelletier, V. Luu-The, S. Li, L. Ren, and F. Labrie
Sex-related Expression of 20{alpha}-hydroxysteroid Dehydrogenase mRNA in the Adult Mouse
J. Histochem. Cytochem., November 1, 2003; 51(11): 1425 - 1436.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1972 by the Society for the Study of Reproduction.