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Biology of Reproduction, Vol 6, 300-309, Copyright © 1972 by Society for the Study of Reproduction
1 Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts 01545 Recently ovulated eggs of mice, rats, and hamsters were treated with hyaluronidase
and subsequently with trypsin or pronase to disperse the corona radiata and zona pellucida.
When these zona-free eggs were inseminated with epididymal or uterine sperm of rats,
epididymal sperm of hamsters or mice in the presence of either heated bovine follicular
fluid or heated blood serum and incubated for 1-12 hr, low proportions of mouse eggs
were penetrated by rat or hamster sperm (1.3-5%), rat eggs by hamster sperm (9%) and
hamster eggs by rat sperm (8.7-26%). Although enlargement of the sperm head was observed in these cases, the transformation of the sperm head into a male pronucleus did not
occur, the activation of eggs to resume the second maturation division was rare, and the
formation of the female pronucleus never occurred. Very high proportions of rat eggs
(95%) or hamster eggs (73%) were penetrated by mouse sperm and polyspermy occurred
as incubation time increased. In these cases, the transformation of many sperm heads
into male pronuclei, the initiation of the second maturation division, and the formation
of a female pronucleus occurred normally. The transformation of mouse sperm heads
into male pronuclei in the rat eggs appeared to be earlier than that of mouse sperm in the
hamster eggs. Although the incorporation or fusion of eggs with foreign sperm may depend on the possibility of sperm capacitation, physiological affinity between the sperm of
one species and the vitellus of another is also an important controlling factor, but the zona
pellucida appears to be the major block for interspecific fertilization.
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