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Biology of Reproduction, Vol 6, 310-318, Copyright © 1972 by Society for the Study of Reproduction

Antigens of the Hamster Ovary and Effects of Anti-Ovary Serum on Eggs

CHARLOTTE L. OWNBY 1, and C. ALEX SHIVERS 1

1 Department of Zoology and Entomology, University of Tennessee, Knoxville, Tennessee 37916


Antisera produced in rabbits against golden hamster ovary were tested on agar-gel diffusion plates against extracts of ovary and 11 other hamster tissues. Seven antigen-antibody systems were observed and, among these, antigens specific to ovary were identified by absorption of the antisera with lyophilized tissues. Tests on antisera absorbed with plasma, small intestine, and both small intestine and lung indicated that several antigens were common to ovary and all additional tissues tested, while others were restricted in their distribution. A minimum of one antigen was specific to the ovary.

When superovulated hamster eggs were treated with unabsorbed, plasma-absorbed, or small intestine-and lung-absorbed antisera, a precipitate formed in the zona pellucida. Treatment of eggs with control sera or antisera absorbed with the immunizing antigen produced no precipitate, suggesting that the precipitate was of immunochemical origin. Formation of the precipitate upon treatment of eggs with small intestine-and lung-absorbed antisera indicated that the antigen was specific to the ovary and may be specific to the zona pellucida.

Trypsin, which usually removes the zona pellucida, failed to do so when eggs were pretreated with unabsorbed or small intestine- and lung-absorbed antisera. However, trypsin was effective in removing the zona from eggs pretreated with ovary-absorbed antisera indicating that an antigen-antibody reaction in the zona pellucida formed a block to the action of trypsin on the zona. The antibody involved was directed against ovary and the antigen was located in the zona pellucida. The possibility that the zona antigen may play a role in penetration of the zona by sperm is discussed.

 Submitted on November 17, 1971




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C. Shivers and B. Dunbar
Autoantibodies to zona pellucida: a possible cause for infertility in women
Science, September 9, 1977; 197(4308): 1082 - 1084.
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C. A. Shivers, A. B. Dudkiewicz, L. E. Franklin, and E. N. Fussell
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Copyright © 1972 by the Society for the Study of Reproduction.