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Biology of Reproduction, Vol 6, 325-332, Copyright © 1972 by Society for the Study of Reproduction

Serum Follicle-Stimulating Hormone in Sheep as Measured by Radioimmunoassay

M. L’HERMITE 1, G. D. NISWENDER 1, L. E. REICHERT Jr. 1, , and A. R. MIDGLEY Jr. 1

1 Department of Pathology, The University of Michigan, Ann Arbor, Michigan 48104 and Department of Biochemistry, Division of Basic Health Sciences, Emory University, Atlanta, Georgia (LER)


Antiovine FSH sera, obtained from 32 different rabbits, were tested with radioiodinated ovine FSH and found to be unsuitable for the development of a specific ovine FSH radioimmunoassay. In contrast, when utilized with labeled human FSH, two antisera appeared to be suitable. Inhibition curves with ovine serum, crude pituitary extract, and other preparations containing FSH were parallel to the standard when analyzed with either antiserum. With the exception of two preparations containing very large amounts of LH, estimates of FSH activity obtained by either radioimmunoassay in preparations containing widely varying amounts of FSH, LH, TSH, GH, and prolactin, were in good agreement with the values obtained by bioassay or predicted on the basis of the extraction procedures employed. When a pituitary homogenate and one of the LH-rich preparations were subjected to polyacrylamide gel electrophoresis, single peaks of radioimmunoassayable FSH activity, completely separated from LH and possessing a mobility comparable to that of highly purified and biologically active FSH, were found for each preparation. Complete recovery of exogenous FSH from serum demonstrated that serum components did not interfere with either assay. Serum concentrations of FSH, expressed in equivalents of NIH-FSH-S4 as ng/ml were: normal males, 60-119; castrated males, 280-975; castrated females, 205-690; females during the cycle except on estrus, 100-160; females on the day of estrus, 180-220. These results suggest that the two antiovine FSH sera can each be used in a heterologous radioimmunoassay system for the specific quantitation of ovine FSH.

Submitted on December 29, 1971




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Copyright © 1972 by the Society for the Study of Reproduction.