| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Articles |
a Department of Biomedical Sciences, Ontario Veterinary College, University of Guelph, Guelph, Ontario,Canada N1G 2W1
Matrix metalloproteinases (MMPs) and tissue inhibitors of MMPs (TIMPs) play very important roles in extracellular matrix (ECM) remodeling in ovarian follicle growth and ovulation. Equine follicles are embedded in cortex that is at the center of the ovary, and they must expand/emigrate to the fossa, the only site in the ovary for ovulation. Therefore, equine ovarian stromal cells (EOSC) are probably involved in ECM remodeling during follicle growth. This study examined whether cultured EOSC synthesize gelatinases and TIMPs, molecules essential for ECM remodeling in other systems. Results showed that cultured EOSC (passage 3–8) had a fibroblast-like morphology and were positive for 
-smooth muscle actin and type I procollagen by immunostaining. Gelatinase A (MMP-2), gelatinase B (MMP-9), TIMP-1, and TIMP-2 were present in EOSC-conditioned medium, and TIMP-3 in ECM of EOSC. Transforming growth factor ß significantly stimulated the activity of gelatinases A and B and TIMP-1 in conditioned medium from EOSC (p < 0.05). Phorbol 12-myristate 13-acetate also significantly stimulated the activity of gelatinases A and B and TIMP-1 in conditioned medium and of TIMP-3 in ECM (p < 0.05). Our results suggest that EOSC produce important components of the ECM remodeling machinery and, therefore, may play a role in the ECM remodeling during follicle growth in this species.
2 Correspondence. FAX: 519 767 1450; bcoomber{at}uoguelph.ca
This article has been cited by other articles:
|
|
 |
|
  D. Rosairo, I. Kuyznierewicz, J. Findlay, and A. Drummond Transforming growth factor-{beta}: its role in ovarian follicle development Reproduction, December 1, 2008; 136(6): 799 - 809. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Starr, T. Dan, K. Minhas, P. E. Shewen, and B. L. Coomber Potential Involvement of Gelatinases and Their Inhibitors in Mannheimia haemolytica Pneumonia in Cattle Infect. Immun., August 1, 2004; 72(8): 4393 - 4400. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-C. Ke, L.-C. Chuang, M.-T. Lee, Y. J. Chen, S.-W. Lin, P. S. Wang, D. M. Stocco, and J.-J. Hwang The Modulatory Role of Transforming Growth Factor {beta}1 and Androstenedione on Follicle-Stimulating Hormone-Induced Gelatinase Secretion and Steroidogenesis in Rat Granulosa Cells Biol Reprod, May 1, 2004; 70(5): 1292 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. E. Curry Jr. and K. G. Osteen The Matrix Metalloproteinase System: Changes, Regulation, and Impact throughout the Ovarian and Uterine Reproductive Cycle Endocr. Rev., August 1, 2003; 24(4): 428 - 465. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Curry Jr., L. Song, and S. E. Wheeler Cellular Localization of Gelatinases and Tissue Inhibitors of Metalloproteinases During Follicular Growth, Ovulation, and Early Luteal Formation in the Rat Biol Reprod, September 1, 2001; 65(3): 855 - 865. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. E. Curry Jr and K. G. Osteen Cyclic Changes in the Matrix Metalloproteinase System in the Ovary and Uterus Biol Reprod, May 1, 2001; 64(5): 1285 - 1296. [Abstract] [Full Text]
|
|
|
|
 |
|
 G Manandhar, R. Moreno, C Simerly, K Toshimori, and G Schatten Contractile apparatus of the normal and abortive cytokinetic cells during mouse male meiosis J. Cell Sci., January 12, 2000; 113(23): 4275 - 4286. [Abstract] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
