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Messenger Ribonucleic Acid in Sheep Endometrium by Increasing Its Stability1
a Department of Animal Science and Center for Animal Biotechnology,
b Institute of Biosciences and Technology, and Department of Veterinary Anatomy and Public Health, Texas A&M University, College Station, Texas 77843-2471
During the preovulatory period, estrogen up-regulates estrogen receptor- (ER) gene expression in endometrium in female mammals of all species examined. The purpose of this study was to determine directly whether estradiol up-regulates ER mRNA by increasing the stability of the message. Endometrial tissue was collected from ovariectomized ewes 18 h after the ewes were injected with 50 µg estradiol. Previous work indicated rapid accumulation of ER mRNA at this time. Estradiol increased uterine weights (to 157 ± 15%) as well as steady-state concentrations of ER (to 309 ± 37%), progesterone receptor (PR; to 165 ± 19%), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH; to 374 ± 32%) mRNAs in endometrium, compared to control levels of 100%. The effects of estradiol on ER mRNA stability in endometrium were measured in explants cultured with the transcription inhibitor 5,6-dichloro-1-ß-D-ribofuranosylbenzimidazole, as well as by labeling RNA in vivo with 4-thiouridine. Both assays indicated that estradiol enhanced ER mRNA stability (half-life increased from 9 h to 
24 h). The estradiol effect was specific, because the stabilities of PR, GAPDH, and c-fos mRNAs were unaffected by treatment. Thus, estradiol up-regulates steady-state concentrations of ER mRNA in endometrium by a novel posttranscriptional mechanism.
2 Correspondence. FAX: 409 862 3399; ning{at}cvm.tamu.edu
3 Current address: Department of Animal and Veterinary Science, 216 Agricultural Science Building, University of Idaho, Moscow, ID 83844-2330.
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