| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Zoology Department, Oxford University, Oxford OX1 3PS, United Kingdom
The purpose of this study was to measure the effect of insulin-like growth factor-2 deficiency on the growth of the mouse conceptus. Initial observations on normal development in the 129J/Sv strain established that wet and dry weights were reduced by 35% when the insulin-like growth factor-2 gene was inactive. The DNA contents were reduced by only 15%. We exchanged the inner cell mass and trophectoderm between mouse blastocysts that had or lacked an active insulin-like growth factor-2 gene. At embryonic Day 16.5, lack of this gene's activity in the derivatives of either tissue decreased the fluid volumes of the exocelomic and amniotic cavities. The wet weights of the "fetal placentas," the yolk sacs, and the fetuses were also decreased. However, the tissue wet weight decrease could not be accounted for by the change in DNA content, indicating that cell-associated biomass had changed. The conclusions are 1) that insulin-like growth factor-2 levels regulate the composition of the fetus and extra-embryonic tissues and 2) that trophectoderm and inner cell mass derivatives cooperate to control extra-cellular fluid volume in the conceptus.
2 Correspondence: C.F. Graham, Zoology Department, South Parks Road, Oxford OX1 3PS, UK. FAX: 01865 271228; chris.graham{at}zoo.ox.ac.uk
This article has been cited by other articles:
|
|
 |
|
  C. Palmieri, P. Loi, G. Ptak, and L.D. Salda REVIEW PAPER: A Review of the Pathology of Abnormal Placentae of Somatic Cell Nuclear Transfer Clone Pregnancies in Cattle, Sheep, and Mice Vet. Pathol., November 1, 2008; 45(6): 865 - 880. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Constancia, E. Angiolini, I. Sandovici, P. Smith, R. Smith, G. Kelsey, W. Dean, A. Ferguson-Smith, C. P. Sibley, W. Reik, et al. Adaptation of nutrient supply to fetal demand in the mouse involves interaction between the Igf2 gene and placental transporter systems PNAS, December 27, 2005; 102(52): 19219 - 19224. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Ravelich, B. H. Breier, S. Reddy, J. A. Keelan, D. N. Wells, A. J. Peterson, and R. S.F. Lee Insulin-Like Growth Factor-I and Binding Proteins 1, 2, and 3 in Bovine Nuclear Transfer Pregnancies Biol Reprod, February 1, 2004; 70(2): 430 - 438. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Reik, M. Constancia, A. Fowden, N. Anderson, W. Dean, A. Ferguson-Smith, B. Tycko, and C. Sibley Regulation of supply and demand for maternal nutrients in mammals by imprinted genes J. Physiol., February 15, 2003; 547(1): 35 - 44. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. L. Burns and A. B. Hassan Cell survival and proliferation are modified by insulin-like growth factor 2 between days 9 and 10 of mouse gestation Development, October 1, 2001; 128(19): 3819 - 3830. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. R. Hill, R. C. Burghardt, K. Jones, C. R. Long, C. R. Looney, T. Shin, T. E. Spencer, J. A. Thompson, Q. A. Winger, and M. E. Westhusin Evidence for Placental Abnormality as the Major Cause of Mortality in First-Trimester Somatic Cell Cloned Bovine Fetuses Biol Reprod, December 1, 2000; 63(6): 1787 - 1794. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
