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Biology of Reproduction 60, 241-250 (1999)
©Copyright 1999 Society for the Study of Reproduction, Inc.

Identification and Characterization of Glycosylation-Dependent Cell Adhesion Molecule 1-Like Protein Expression in the Ovine Uterus

Thomas E. Spencer1,a, Frank F. Bartolb, Fuller W. Bazera, Gregory A. Johnsona, and Margaret M. Joycea

a Center for Animal Biotechnology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University, College Station, Texas 77843-2471 b Department of Animal Science, Auburn University, Auburn, Alabama 36849

Glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) is an endothelial glycoprotein secreted in lymph nodes that serves as a ligand for leukocyte cell surface selectin and mediates lymphocyte extravasation. In the present studies, rabbit anti-rat GlyCAM-1 IgG was used in immunochemical analyses of GlyCAM-1-like protein in the ovine uterus. In cyclic ewes, GlyCAM-1 expression increased in the endometrial luminal epithelium (LE) and shallow glandular epithelium (cGE) between Days 1 and 5 and then decreased between Days 11 and 15. In pregnant ewes, GlyCAM-1 in the LE and cGE was low on Days 11 and 13, increased on Day 15, and was abundant on Days 17 and 19. Immunoreactive GlyCAM-1 was also detected in the conceptus trophectoderm on Days 13–19. Staining for GlyCAM-1 in the smooth muscle of the vasculature and myometrium was constitutive, and no staining was detected in the stroma. An immunoreactive protein of ~45 kDa was identified in endometrial extracts and uterine flushings from cyclic and pregnant ewes. In pregnant ewes, the relative amount of immunoreactive GlyCAM-1 in uterine flushings was low on Days 11 and 13 but high on Days 15 and 17. Results suggest that a GlyCAM-1-like protein may be a secretory product of the endometrial epithelium and/or conceptus trophectoderm. Patterns of distribution observed for immunoreactive GlyCAM-1-like protein in the endometrial epithelium, combined with proposed functions for lymphoid GlyCAM-1, suggest that this mucin glycoprotein may be involved in conceptus-maternal interactions during the periimplantation period of pregnancy in sheep.

1 Correspondence: Thomas E. Spencer, Center for Animal Biotechnology, Albert B. Alkek Institute of Biosciences and Technology, 444 Kleberg Center, Texas A&M University, College Station, TX 77843–2471. FAX: 409 862 2662; tspencer{at}ansc.tamu.edu




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Copyright © 1999 by the Society for the Study of Reproduction.