Biol Reprod Lalor Postdoctoral Fellowships -- Application Deadline January 15, 2009
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Spencer, T. E.
Right arrow Articles by Joyce, M. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Spencer, T. E.
Right arrow Articles by Joyce, M. M.
Agricola
Right arrow Articles by Spencer, T. E.
Right arrow Articles by Joyce, M. M.
Biology of Reproduction 60, 241-250 (1999)
©Copyright 1999 Society for the Study of Reproduction, Inc.

Identification and Characterization of Glycosylation-Dependent Cell Adhesion Molecule 1-Like Protein Expression in the Ovine Uterus

Thomas E. Spencer1,a, Frank F. Bartolb, Fuller W. Bazera, Gregory A. Johnsona, and Margaret M. Joycea

a Center for Animal Biotechnology, Albert B. Alkek Institute of Biosciences and Technology, Texas A&M University, College Station, Texas 77843-2471 b Department of Animal Science, Auburn University, Auburn, Alabama 36849

Glycosylation-dependent cell adhesion molecule 1 (GlyCAM-1) is an endothelial glycoprotein secreted in lymph nodes that serves as a ligand for leukocyte cell surface selectin and mediates lymphocyte extravasation. In the present studies, rabbit anti-rat GlyCAM-1 IgG was used in immunochemical analyses of GlyCAM-1-like protein in the ovine uterus. In cyclic ewes, GlyCAM-1 expression increased in the endometrial luminal epithelium (LE) and shallow glandular epithelium (cGE) between Days 1 and 5 and then decreased between Days 11 and 15. In pregnant ewes, GlyCAM-1 in the LE and cGE was low on Days 11 and 13, increased on Day 15, and was abundant on Days 17 and 19. Immunoreactive GlyCAM-1 was also detected in the conceptus trophectoderm on Days 13–19. Staining for GlyCAM-1 in the smooth muscle of the vasculature and myometrium was constitutive, and no staining was detected in the stroma. An immunoreactive protein of ~45 kDa was identified in endometrial extracts and uterine flushings from cyclic and pregnant ewes. In pregnant ewes, the relative amount of immunoreactive GlyCAM-1 in uterine flushings was low on Days 11 and 13 but high on Days 15 and 17. Results suggest that a GlyCAM-1-like protein may be a secretory product of the endometrial epithelium and/or conceptus trophectoderm. Patterns of distribution observed for immunoreactive GlyCAM-1-like protein in the endometrial epithelium, combined with proposed functions for lymphoid GlyCAM-1, suggest that this mucin glycoprotein may be involved in conceptus-maternal interactions during the periimplantation period of pregnancy in sheep.

1 Correspondence: Thomas E. Spencer, Center for Animal Biotechnology, Albert B. Alkek Institute of Biosciences and Technology, 444 Kleberg Center, Texas A&M University, College Station, TX 77843–2471. FAX: 409 862 2662; tspencer{at}ansc.tamu.edu




This article has been cited by other articles:


Home page
 Biol. Reprod.Home page
G. Song, M. C. Satterfield, J. Kim, F. W. Bazer, and T. E. Spencer
Gastrin-Releasing Peptide (GRP) in the Ovine Uterus: Regulation by Interferon Tau and Progesterone
Biol Reprod, August 1, 2008; 79(2): 376 - 386.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. Kim, G. Song, H. Gao, J. L. Farmer, M. C. Satterfield, R. C. Burghardt, G. Wu, G. A. Johnson, T. E. Spencer, and F. W. Bazer
Insulin-Like Growth Factor II Activates Phosphatidylinositol 3-Kinase-Protooncogenic Protein Kinase 1 and Mitogen-Activated Protein Kinase Cell Signaling Pathways, and Stimulates Migration of Ovine Trophectoderm Cells
Endocrinology, June 1, 2008; 149(6): 3085 - 3094.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Song, J. Kim, F. W. Bazer, and T. E. Spencer
Progesterone and Interferon Tau Regulate Hypoxia-Inducible Factors in the Endometrium of the Ovine Uterus
Endocrinology, April 1, 2008; 149(4): 1926 - 1934.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. C. Satterfield, K. A. Dunlap, K. Hayashi, R. C. Burghardt, T. E. Spencer, and F. W. Bazer
Tight and Adherens Junctions in the Ovine Uterus: Differential Regulation by Pregnancy and Progesterone
Endocrinology, August 1, 2007; 148(8): 3922 - 3931.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
G. Song, F. W Bazer, and T. E Spencer
Pregnancy and interferon tau regulate RSAD2 and IFIH1 expression in the ovine uterus
Reproduction, January 1, 2007; 133(1): 285 - 295.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. C. Satterfield, F. W. Bazer, and T. E. Spencer
Progesterone Regulation of Preimplantation Conceptus Growth and Galectin 15 (LGALS15) in the Ovine Uterus
Biol Reprod, August 1, 2006; 75(2): 289 - 296.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Song, T. E. Spencer, and F. W. Bazer
Progesterone and Interferon-{tau} Regulate Cystatin C in the Endometrium
Endocrinology, July 1, 2006; 147(7): 3478 - 3483.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
G. Song, F. W. Bazer, G. F. Wagner, and T. E. Spencer
Stanniocalcin (STC) in the Endometrial Glands of the Ovine Uterus: Regulation by Progesterone and Placental Hormones
Biol Reprod, May 1, 2006; 74(5): 913 - 922.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
J. J Muniz, M. M Joyce, J. D Taylor II, J. R Burghardt, R. C Burghardt, and G. A Johnson
Glycosylation dependent cell adhesion molecule 1-like protein and L-selectin expression in sheep interplacentomal and placentomal endometrium.
Reproduction, April 1, 2006; 131(4): 751 - 761.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. G. W. Fleming, T. E. Spencer, S. H. Safe, and F. W. Bazer
Estrogen Regulates Transcription of the Ovine Oxytocin Receptor Gene through GC-Rich SP1 Promoter Elements
Endocrinology, February 1, 2006; 147(2): 899 - 911.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
G. Song, T. E. Spencer, and F. W. Bazer
Cathepsins in the Ovine Uterus: Regulation by Pregnancy, Progesterone, and Interferon Tau
Endocrinology, November 1, 2005; 146(11): 4825 - 4833.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
C A. Gray, K. A Dunlap, R. C Burghardt, and T. E Spencer
Galectin-15 in ovine uteroplacental tissues
Reproduction, August 1, 2005; 130(2): 231 - 240.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. M. Joyce, F. J. White, R. C. Burghardt, J. J. Muniz, T. E. Spencer, F. W. Bazer, and G. A. Johnson
Interferon Stimulated Gene 15 Conjugates to Endometrial Cytosolic Proteins and Is Expressed at the Uterine-Placental Interface throughout Pregnancy in Sheep
Endocrinology, February 1, 2005; 146(2): 675 - 684.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
T. E Spencer, G. A Johnson, F. W Bazer, and R. C Burghardt
Implantation mechanisms: insights from the sheep
Reproduction, December 1, 2004; 128(6): 657 - 668.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
J. Hu, X. Zhang, W. B. Nothnick, and T. E. Spencer
Matrix Metalloproteinases and Their Tissue Inhibitors in the Developing Neonatal Mouse Uterus
Biol Reprod, November 1, 2004; 71(5): 1598 - 1604.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
C. A. Gray, D. L. Adelson, F. W. Bazer, R. C. Burghardt, E. N. T. Meeusen, and T. E. Spencer
Discovery and characterization of an epithelial-specific galectin in the endometrium that forms crystals in the trophectoderm
PNAS, May 25, 2004; 101(21): 7982 - 7987.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
T. E. Spencer and F. W. Bazer
Uterine and placental factors regulating conceptus growth in domestic animals
J Anim Sci, January 1, 2004; 82(13_suppl): E4 - 13.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. D. Carpenter, C. A. Gray, S. Noel, A. Gertler, F. W. Bazer, and T. E. Spencer
Prolactin Regulation of Neonatal Ovine Uterine Gland Morphogenesis
Endocrinology, January 1, 2003; 144(1): 110 - 120.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
M. Palmarini, C. A. Gray, K. Carpenter, H. Fan, F. W. Bazer, and T. E. Spencer
Expression of Endogenous Betaretroviruses in the Ovine Uterus: Effects of Neonatal Age, Estrous Cycle, Pregnancy, and Progesterone
J. Virol., December 1, 2001; 75(23): 11319 - 11327.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
C. A. Gray, K. M. Taylor, W. S. Ramsey, J. R. Hill, F. W. Bazer, F. F. Bartol, and T. E. Spencer
Endometrial Glands Are Required for Preimplantation Conceptus Elongation and Survival
Biol Reprod, June 1, 2001; 64(6): 1608 - 1613.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
G. A. Johnson, M. D. Stewart, C. Allison Gray, Y. Choi, R. C. Burghardt, L.-Y. Yu-Lee, F. W. Bazer, and T. E. Spencer
Effects of the Estrous Cycle, Pregnancy, and Interferon Tau on 2',5'-Oligoadenylate Synthetase Expression in the Ovine Uterus
Biol Reprod, May 1, 2001; 64(5): 1392 - 1399.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
Z. Hou, J. P. Bailey, A. J. Vomachka, M. Matsuda, J. A. Lockefeer, and N. D. Horseman
Glycosylation-Dependent Cell Adhesion Molecule 1 (GlyCAM 1) Is Induced by Prolactin and Suppressed by Progesterone in Mammary Epithelium
Endocrinology, November 1, 2000; 141(11): 4278 - 4283.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. D. Stewart, G. A. Johnson, C. A. Gray, R. C. Burghardt, L. A. Schuler, M. M. Joyce, F. W. Bazer, and T. E. Spencer
Prolactin Receptor and Uterine Milk Protein Expression in the Ovine Endometrium During the Estrous Cycle and Pregnancy
Biol Reprod, June 1, 2000; 62(6): 1779 - 1789.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
C. Chen, T. E. Spencer, and F. W. Bazer
Expression of Hepatocyte Growth Factor and Its Receptor c-metin the Ovine Uterus
Biol Reprod, June 1, 2000; 62(6): 1844 - 1850.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
G. A. Johnson, T. E. Spencer, R. C. Burghardt, K. M. Taylor, C. A. Gray, and F. W. Bazer
Progesterone Modulation of Osteopontin Gene Expression in the Ovine Uterus
Biol Reprod, May 1, 2000; 62(5): 1315 - 1321.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
G. A. Johnson, T. E. Spencer, R. C. Burghardt, M. M. Joyce, and F. W. Bazer
Interferon-Tau and Progesterone Regulate Ubiquitin Cross-Reactive Protein Expression in the Ovine Uterus
Biol Reprod, March 1, 2000; 62(3): 622 - 627.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
G. A. Johnson, R. C. Burghardt, G. R. Newton, F. W. Bazer, and T. E. Spencer
Development and Characterization of Immortalized Ovine Endometrial Cell Lines
Biol Reprod, November 1, 1999; 61(5): 1324 - 1330.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
T. E. Spencer, A. G. Stagg, M. M. Joyce, G. Jenster, C. G. Wood, F. W. Bazer, A. A. Wiley, and F. F. Bartol
Discovery and Characterization of Endometrial Epithelial Messenger Ribonucleic Acids Using the Ovine Uterine Gland Knockout Model
Endocrinology, September 1, 1999; 140(9): 4070 - 4080.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1999 by the Society for the Study of Reproduction.