HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal My Folders Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Skarzynski, D. J Articles by Okuda, K. Search for Related Content PubMed PubMed Citation Articles by Skarzynski, D. J Articles by Okuda, K. Agricola Articles by Skarzynski, D. J Articles by Okuda, K.

Noradrenaline Stimulates the Production of Prostaglandin F₂ in Cultured Bovine Endometrial Cells¹

^a Laboratory of Reproductive Endocrinology, Faculty of Agriculture, Okayama University, Okayama 700-8530, Japan ^b Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, 10-718 Olsztyn-Kortowo, Poland

The stimulatory effect of noradrenaline (NA) as well as oxytocin (OT) on bovine endometrial prostaglandin (PG) F₂ production, and the intracellular mechanisms of their actions, were investigated in cultured bovine endometrial cells (a mixture of epithelial, stromal, and glandular cells). The cells were cultured in Dulbecco's Modified Eagle's medium and Ham's F-12 medium (1:1 [v:v]) with 10% calf serum. When the cells reached confluence, the culture medium was replaced with fresh medium with 0.1% BSA and various doses of NA (10^-8–10^-4 M). NA stimulated PGF₂ production in a dose-dependent manner (p < 0.05). To evaluate the intracellular mechanisms of NA and OT actions, the cells were treated with forskolin (an activator of adenylate cyclase), phorbol 12-myristate 13-acetate (PMA, an activator of protein kinase [PK] C), Rp-cAMP (a competitive cAMP antagonist and an inhibitor of PKA), U-73122 (an inhibitor of phospholipase [PL] C), or anthranilic acid (ACA, an inhibitor of PLA₂). Forskolin and PMA stimulated PGF₂ production in a dose-dependent manner (p < 0.05). Rp-cAMP completely inhibited (p < 0.001) the NA-induced, but not the OT-induced, PGF₂ production. Although U-73122 inhibited only OT-induced PGF₂ production (p < 0.001), ACA completely stopped the actions of NA and OT. The overall results indicate that NA as well as OT is involved in the regulation of the endometrial PGF₂ production in cattle and that the stimulatory effects of NA and OT on PGF₂ production are mediated via the PKA and PKC pathways, respectively.

¹ This research was supported by the Polish National Research Council (KBN 5 P06K 027 13), Grants-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan (No. 96346), the Japanese-German Cooperative Science Promotion Program of the Japanese Society for the Promotion of Science (JSPS), and a grant for the specific research "The study of the development of organisms effective to environmental conservation for human life" at Okayama University in 1998–1999. D.J.S. is a postdoctoral fellow supported by JSPS.

² Correspondence. FAX: 81 86 251 8388; kokuda{at}cc.okayama-u.ac.jp

This article has been cited by other articles:

				S. R. Hansson, B. Bottalico, V. Noskova, and B. Casslen Monoamine transporters in human endometrium and decidua Hum. Reprod. Update, March 1, 2009; 15(2): 249 - 260. [Abstract] [Full Text] [PDF]