| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
a Reproductive Medicine Laboratory, Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, Edinburgh EH3 9EW, United Kingdom
A new concept in reproductive endocrinology is that the status of the ovary as a glucocorticoid target organ alters with follicular development. Evidence for a physiological role of glucocorticoids in the regulation of ovarian folliculogenesis has been strengthened by the discovery that 11ß-hydroxysteroid dehydrogenase (11ßHSD) mRNA expression in human granulosa cells is developmentally regulated. In this study, we quantified the pattern of expression and investigated the cellular location of 11ßHSD type 1 (11ßHSD1), 11ßHSD type 2 (11ßHSD2), glucocorticoid receptor (GR), and mineralocorticoid receptor (MR) mRNAs during follicular maturation in rat ovary. Immature female rats received treatment with eCG to induce preovulatory follicular development or eCG followed by hCG to induce luteinization. 11ßHSD1, 11ßHSD2, GR, and MR mRNAs were all detectable by ribonuclease protection assay in ovarian total RNA. Treatment with eCG alone caused an ~8-fold increase in the ovarian level of 11ßHSD1 mRNA, which rose to ~30-fold after additional treatment with hCG. Equine CG alone did not measurably affect the ovarian 11ßHSD2 mRNA level, but additional treatment with hCG reduced it to 34% of the control level. Expression of GR mRNA was unchanged by any gonadotropin treatment, while MR mRNA was down-regulated. A similar pattern of 11ßHSD1, 11ßHSD2, GR, and MR mRNA expression was observed in isolated granulosa cells. These results provide direct experimental evidence that 11ßHSD genes are gonadotropically regulated in the rat ovary, including granulosa cells, and are consistent with a shift in glucocorticoid metabolism from inactivation (due to oxidation by 11ßHSD2) to activation (reduction by 11ßHSD1) during hCG-induced granulosa cell luteinization.
2 Correspondence: S. Hillier, Reproductive Medicine Laboratory, Department of Obstetrics and Gynaecology, University of Edinburgh, Centre for Reproductive Biology, 37 Chalmers Street, Edinburgh EH3 9EW, UK. FAX: 44 0131 228 5891; sgh{at}srv1.meded.ac.uk
This article has been cited by other articles:
|
|
 |
|
  M. Myers, M. C. Lamont, S. van den Driesche, N. Mary, K. J. Thong, S. G. Hillier, and W. C. Duncan Role of Luteal Glucocorticoid Metabolism during Maternal Recognition of Pregnancy in Women Endocrinology, December 1, 2007; 148(12): 5769 - 5779. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. G Hillier Diamonds are forever: the cortisone legacy J. Endocrinol., October 1, 2007; 195(1): 1 - 6. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L M Thurston, D R E Abayasekara, and A E Michael 11{beta}-Hydroxysteroid dehydrogenase expression and activities in bovine granulosa cells and corpora lutea implicate corticosteroids in bovine ovarian physiology J. Endocrinol., May 1, 2007; 193(2): 299 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. C. Jonas, C. Chandras, D. R. E. Abayasekara, and A. E. Michael Role for Prostaglandins in the Regulation of Type 1 11{beta}-Hydroxysteroid Dehydrogenase in Human Granulosa-Lutein Cells Endocrinology, December 1, 2006; 147(12): 5865 - 5872. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ozaki, M. Higuchi, C. Miura, S. Yamaguchi, Y. Tozawa, and T. Miura Roles of 11{beta}-Hydroxysteroid Dehydrogenase in Fish Spermatogenesis Endocrinology, November 1, 2006; 147(11): 5139 - 5146. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. S. Gilmour, A. E. Coutinho, J.-F. Cailhier, T. Y. Man, M. Clay, G. Thomas, H. J. Harris, J. J. Mullins, J. R. Seckl, J. S. Savill, et al. Local amplification of glucocorticoids by 11beta-hydroxysteroid dehydrogenase type 1 promotes macrophage phagocytosis of apoptotic leukocytes. J. Immunol., June 15, 2006; 176(12): 7605 - 7611. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L.M. Thurston, D.P. Norgate, K.C. Jonas, L. Gregory, P.J. Wood, B.A. Cooke, and A.E. Michael Ovarian modulators of type 1 11{beta}-hydroxysteroid dehydrogenase (11{beta}HSD) activity and intra-follicular cortisol:cortisone ratios correlate with the clinical outcome of IVF Hum. Reprod., August 1, 2003; 18(8): 1603 - 1612. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Kusakabe, I. Nakamura, and G. Young 11{beta}-Hydroxysteroid Dehydrogenase Complementary Deoxyribonucleic Acid in Rainbow Trout: Cloning, Sites of Expression, and Seasonal Changes in Gonads Endocrinology, June 1, 2003; 144(6): 2534 - 2545. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.J. Peluso, T. Bremner, G. Fernandez, A. Pappalardo, and B.A. White Expression Pattern and Role of a 60-Kilodalton Progesterone Binding Protein in Regulating Granulosa Cell Apoptosis: Involvement of the Mitogen-Activated Protein Kinase Cascade Biol Reprod, January 1, 2003; 68(1): 122 - 128. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Y. Arai, K. Tsuchida, K. Uehara, K. Taya, and H. Sugino Characterization of Rat Follistatin-Related Gene: Effects of Estrous Cycle Stage and Pregnancy on Its Messenger RNA Expression in Rat Reproductive Tissues Biol Reprod, January 1, 2003; 68(1): 199 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Sasson and A. Amsterdam Stimulation of Apoptosis in Human Granulosa Cells from in Vitro Fertilization Patients and Its Prevention by Dexamethasone: Involvement of Cell Contact and Bcl-2 Expression J. Clin. Endocrinol. Metab., July 1, 2002; 87(7): 3441 - 3451. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.J. Peluso, G. Fernandez, A. Pappalardo, and B.A. White Characterization of a Putative Membrane Receptor for Progesterone in Rat Granulosa Cells Biol Reprod, July 1, 2001; 65(1): 94 - 101. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Y. K. Yong, K. J. Thong, R. Andrew, B. R. Walker, and S. G. Hillier Development-Related Increase in Cortisol Biosynthesis by Human Granulosa Cells J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4728 - 4733. [Abstract] [Full Text]
|
|
|
|
 |
|
 C.L. Chaffin, G.A. Dissen, and R.L. Stouffer Hormonal regulation of steroidogenic enzyme expression in granulosa cells during the peri-ovulatory interval in monkeys Mol. Hum. Reprod., January 1, 2000; 6(1): 11 - 18. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
